Abstract
To investigate UV light response mechanisms in higher plants, we isolated a UV light-sensitive mutant, rev3-1, in Arabidopsis. The root growth of rev3-1 was inhibited after UV-B irradiation under both light and dark conditions. We found that chromosome 1 of rev3-1 was broken at a minimum of three points, causing chromosome inversion and translocation. A gene disrupted by this rearrangement encoded the catalytic subunit of DNA polymerase zeta (AtREV3), which is thought to be involved in translesion synthesis. The rev3-1 seedlings also were sensitive to gamma-rays and mitomycin C, which are known to inhibit DNA replication. Incorporation of bromodeoxyuridine after UV-B irradiation was less in rev3-1 than in the wild type. These results indicate that UV light-damaged DNA interrupted DNA replication in the rev3-1 mutant, leading to the inhibition of cell division and root elongation.
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Claims 7
Claim · model-written summary
UV-B inhibited rev3-1 root growth under light and dark conditions.
- Taxon
- Arabidopsis
Arabidopsist0 - Material
- rev3-1e0
- Treatment
- UV-B irradiatione0
- Outcome
- root growthe0
- Direction
- inhibitede0
- Qualifier
- under both light and dark conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The root growth of rev3-1 was inhibited after UV-B irradiation under both light and dark conditions.”
t0“Arabidopsis”
Claim · model-written summary
rev3-1 chromosome 1 had at least three breaks causing inversion and translocation.
- Taxon
- ArabidopsisArabidopsist0
- Material
- rev3-1e0
- Outcome
- chromosome 1 of rev3-1 was brokene0
- Value
- a minimum of three pointse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“chromosome 1 of rev3-1 was broken at a minimum of three points, causing chromosome inversion and translocation.”
t0“Arabidopsis”
Claim · model-written summary
The disrupted gene encoded the catalytic subunit of DNA polymerase zeta.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- AtREV3e0
- Outcome
- encoded the catalytic subunit of DNA polymerase zetae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A gene disrupted by this rearrangement encoded the catalytic subunit of DNA polymerase zeta (AtREV3)”
t0“Arabidopsis”
Claim · model-written summary
rev3-1 seedlings were sensitive to gamma-rays.
- Taxon
- ArabidopsisArabidopsist0
- Material
- rev3-1e0
- Treatment
- gamma-rayse0
- Outcome
- sensitive to gamma-rayse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rev3-1 seedlings also were sensitive to gamma-rays and mitomycin C, which are known to inhibit DNA replication.”
t0“Arabidopsis”
Claim · model-written summary
rev3-1 seedlings were sensitive to mitomycin C.
- Taxon
- ArabidopsisArabidopsist0
- Material
- rev3-1e0
- Treatment
- mitomycin Ce0
- Outcome
- sensitive to gamma-rays and mitomycin Ce0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rev3-1 seedlings also were sensitive to gamma-rays and mitomycin C, which are known to inhibit DNA replication.”
t0“Arabidopsis”
Claim · model-written summary
After UV-B, rev3-1 incorporated less bromodeoxyuridine than wild type.
- Taxon
- ArabidopsisArabidopsist0
- Material
- rev3-1e0
- Treatment
- UV-B irradiatione0
- Outcome
- Incorporation of bromodeoxyuridinee0
- Direction
- lesse0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Incorporation of bromodeoxyuridine after UV-B irradiation was less in rev3-1 than in the wild type.”
t0“Arabidopsis”
Claim · model-written summary
Authors infer damaged DNA interrupted replication in rev3-1, inhibiting division and root elongation.
- Taxon
- ArabidopsisArabidopsist0
- Material
- rev3-1e0
- Outcome
- inhibition of cell division and root elongatione0
- Qualifier
- UV light-damaged DNA interrupted DNA replicatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UV light-damaged DNA interrupted DNA replication in the rev3-1 mutant, leading to the inhibition of cell division and root elongation.”
t0“Arabidopsis”
The same questions in other papers
uv · growth · ArabidopsisPositive and negative results
6 papers in the same direction as this paper · 4 in the opposite direction
- negative
- positive
- positive
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.