Abstract
The maturation of soybean (Glycine max L. Merr.) somatic embryos was characterized. Maturation was assayed by evaluating the ability of somatic embryos to make the transition to a plantlet through a germination-like process. Somatic embryos were organized from cotyledons of immature soybean embryos. Maturation of somatic embryos occurred on a Murashige-Skoog basal medium supplemented with activated charcoal and 0.28 molar sucrose. After 8 weeks on this medium, somatic embryos exhibited vigorous, high frequency development to plantlets. The "germination" frequency (conversion) of somatic embryos, and plantlet recovery frequency varied concurrently with maturation period. Conversion and plant recovery required no exogenous growth regulators. Desiccation of immature somatic embryos under controlled humidity regimes resulted in increased frequency of conversion of immature somatic embryos. Morphological abnormalities appeared in the somatic embryos, but few were detrimental to conversion velocity. There was little effect of genotype on conversion velocity or frequency.
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Claims 8
Claim · model-written summary
Soybean somatic embryos matured on charcoal and sucrose medium.
- Taxon
- soybean
Glycine maxt0 - Treatment
- Murashige-Skoog basal medium supplemented with activated charcoal and 0.28 molar sucrosee0
- Outcome
- Maturation of somatic embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maturation of somatic embryos occurred on a Murashige-Skoog basal medium supplemented with activated charcoal and 0.28 molar sucrose.”
t0“Soybean”
Claim · model-written summary
After eight weeks on the medium, somatic embryos developed into plantlets at high frequency.
- Taxon
- soybeanGlycine maxt0
- Outcome
- high frequency development to plantletse0
- Qualifier
- After 8 weeks on this mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After 8 weeks on this medium, somatic embryos exhibited vigorous, high frequency development to plantlets.”
t0“Soybean”
Claim · model-written summary
Conversion and plantlet recovery frequencies varied with maturation period.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- maturation periode0
- Outcome
- "germination" frequency (conversion) of somatic embryos, and plantlet recovery frequencye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The "germination" frequency (conversion) of somatic embryos, and plantlet recovery frequency varied concurrently with maturation period.”
t0“Soybean”
Claim · model-written summary
Conversion and plantlet recovery occurred without exogenous growth regulators.
- Taxon
- soybeanGlycine maxt0
- Outcome
- Conversion and plant recoverye0
- Qualifier
- no exogenous growth regulatorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Conversion and plant recovery required no exogenous growth regulators.”
t0“Soybean”
Claim · model-written summary
Controlled desiccation increased conversion frequency of immature embryos.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Desiccation of immature somatic embryos under controlled humidity regimese0
- Outcome
- frequency of conversion of immature somatic embryose0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Desiccation of immature somatic embryos under controlled humidity regimes resulted in increased frequency of conversion of immature somatic embryos.”
t0“Soybean”
Claim · model-written summary
Somatic embryos showed morphological abnormalities.
- Taxon
- soybeanGlycine maxt0
- Outcome
- Morphological abnormalitiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Morphological abnormalities appeared in the somatic embryos”
t0“Soybean”
Claim · model-written summary
Few morphological abnormalities impeded conversion velocity.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- fewe0
- Outcome
- conversion velocitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“few were detrimental to conversion velocity.”
t0“Soybean”
Claim · model-written summary
Genotype had little effect on conversion velocity or frequency.
- Taxon
- soybeanGlycine maxt0
- Material
- genotypee0
- Outcome
- conversion velocity or frequencye0
- Direction
- little effecte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There was little effect of genotype on conversion velocity or frequency.”
t0“Soybean”
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