Abstract
Abstract Self-incompatibility is a widespread mechanism in flowering plants which prevents self-fertilisation and promotes out-crossing. In this investigation, self-compatible and self-incompatible cultivars of Petunia hybrida Vilm. were discriminated based on the study of pollen tube growth in vivo, seed set percent and index of self-incompatibility (ISI) after self- and cross-pollination. After 48 hours of self- and cross- pollination, the pollen tube growth was studied by aniline blue staining and fluorescent microscopy. Fluorescent microscopy studies showed normal germination and growth of pollen tubes on stigmas but some abnormalities of pollen tube growth were observed in self-pollinated styles in some cultivars. These abnormalities include pollen tube growth inhibition in 2/3 upper part of the style, variable pollen tube diameter in middle and lower part of the style and irregular deposition of callose plugs. ISI studies showed a significant decrease in the seed set of self-pollinated pistils compared with cross-pollinated pistils in some cultivars. The self-pollinated cultivars contain abnormalities in pollen tube growth after self-pollination as well as those with decreasing ISI was defined as self-incompatible in our study.
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Claims 6
Claim · model-written summary
Petunia hybrida pollen tubes germinated and grew normally on stigmas after pollination.
- Taxon
- Petunia hybrida
Petunia ×hybridae1 - Organ / stage
- stigmase0
- Outcome
- normal germination and growth of pollen tubes on stigmase0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fluorescent microscopy studies showed normal germination and growth of pollen tubes on stigmas”
e1“Petunia hybrida Vilm.”
Claim · model-written summary
In some self-pollinated cultivars, pollen tube growth was inhibited in the upper two thirds of the style.
- Taxon
- Petunia hybridaPetunia ×hybridae2
- Organ / stage
- stylee0
- Outcome
- pollen tube growth inhibitione0
- Value
- 2/3e0
- Qualifier
- self-pollinated styles in some cultivarse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pollen tube growth inhibition in 2/3 upper part of the style”
e1“some abnormalities of pollen tube growth were observed in self-pollinated styles in some cultivars”
e2“Petunia hybrida Vilm.”
Claim · model-written summary
Some self-pollinated cultivars had variable pollen tube diameter in the middle and lower style.
- Taxon
- Petunia hybridaPetunia ×hybridae2
- Organ / stage
- stylee0
- Outcome
- variable pollen tube diametere0
- Qualifier
- self-pollinated styles in some cultivarse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“variable pollen tube diameter in middle and lower part of the style”
e1“some abnormalities of pollen tube growth were observed in self-pollinated styles in some cultivars”
e2“Petunia hybrida Vilm.”
Claim · model-written summary
Some self-pollinated cultivars had irregular callose plug deposition.
- Taxon
- Petunia hybridaPetunia ×hybridae2
- Outcome
- irregular deposition of callose plugse0
- Qualifier
- self-pollinated styles in some cultivarse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“irregular deposition of callose plugs”
e1“some abnormalities of pollen tube growth were observed in self-pollinated styles in some cultivars”
e2“Petunia hybrida Vilm.”
Claim · model-written summary
Seed set was significantly lower in self-pollinated than cross-pollinated pistils in some cultivars.
- Taxon
- Petunia hybridaPetunia ×hybridae1
- Organ / stage
- pistilse0
- Treatment
- self-pollinated pistilse0
- Outcome
- seed sete0
- Direction
- decreasee0
- Qualifier
- in some cultivarse0
- Comparator
- cross-pollinated pistilse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ISI studies showed a significant decrease in the seed set of self-pollinated pistils compared with cross-pollinated pistils in some cultivars”
e1“Petunia hybrida Vilm.”
Claim · model-written summary
Cultivars with abnormal pollen tube growth after self-pollination and decreasing ISI were defined as self-incompatible.
- Taxon
- Petunia hybridaPetunia ×hybridae1
- Outcome
- self-incompatiblee0
- Qualifier
- abnormalities in pollen tube growth after self-pollinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The self-pollinated cultivars contain abnormalities in pollen tube growth after self-pollination as well as those with decreasing ISI was defined as self-incompatible in our study”
e1“Petunia hybrida Vilm.”
The same questions in other papers
pollination · yield · Petunia x hybrida (garden petunia)Positive and negative results
2 in the opposite direction
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.