Abstract
When dhurrin [ p ‐hydroxy‐(S)‐mandelonitrile‐β‐D‐glucoside], the cyanogenlc glucoside of sorghum [ Sorghum bicolor (L.) Moench], is hydrolyzed by autoclaving, p ‐hydroxybenzaldehyde ( p ‐HB) is released. The spectrophotometric determination of p HB concentration in autoclaved sorghum leaf extracts provides a measure of the hydrocyanic acid potential (HCN‐p) of leaf tissue. Extracts of field‐grown sorghumle aves contained substances that interfered with this procedure, but ether extraction effectively separated p ‐HB from these interfering materials. We observed that when flag leaf tissue from field‐grown sorghum was dried at 75°C and then autoclaved, HCN‐pva lues were about three times as high as those based on tissue that was autoclaved without drying. Investigations of this apparent enhancement supported the conclusion that when fresh field‐grown sorghum leaf tissue was autoclaved, dhurrin was extensively altered or lost, but neither p ‐HB nor HCN was produced. Drying the tissue at 75°C prior to autoclaving effectively reduced this loss. Inclusion of tissue drying and ether extraction steps in the spectrophotometric assay made this procedure, which was designed for use with sorghumse edlings, satisfactory for use with field‐grown sorghum leaves.
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Claims 5
Claim · model-written summary
Ether extraction separated p-HB from assay-interfering substances in field-grown sorghum leaves.
- Treatment
- ether extractione0
- Outcome
- p ‐HB from these interfering materialse0
- Comparator
- interfering materialse0
SorghumOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sorghum Leaf Tissue”
e0“Extracts of field‐grown sorghumle aves contained substances that interfered with this procedure, but ether extraction effectively separated p ‐HB from these interfering materials.”
Claim · model-written summary
Drying at 75°C before autoclaving yielded about threefold higher apparent HCN potential.
- Organ / stage
- flag leaf tissuee0
- Treatment
- dried at 75°C and then autoclavede0
- Outcome
- HCN‐pva luese0
- Direction
- about three times as highe0
- Value
- about three timese0
- Comparator
- tissue that was autoclaved without dryinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when flag leaf tissue from field‐grown sorghum was dried at 75°C and then autoclaved, HCN‐pva lues were about three times as high as those based on tissue that was autoclaved without drying.”
Claim · model-written summary
Autoclaving fresh field-grown sorghum leaf tissue altered or lost dhurrin without producing p-HB or HCN.
- Organ / stage
- leaf tissuee0
- Treatment
- autoclavede0
- Outcome
- dhurrin was extensively altered or lost, but neither p ‐HB nor HCN was producede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when fresh field‐grown sorghum leaf tissue was autoclaved, dhurrin was extensively altered or lost, but neither p ‐HB nor HCN was produced.”
Claim · model-written summary
Drying tissue at 75°C before autoclaving reduced dhurrin loss.
- Treatment
- Drying the tissue at 75°C prior to autoclavinge0
- Outcome
- losse0
- Direction
- reducede0
- Comparator
- fresh field‐grown sorghum leaf tissue was autoclavede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Drying the tissue at 75°C prior to autoclaving effectively reduced this loss.”
e1“when fresh field‐grown sorghum leaf tissue was autoclaved, dhurrin was extensively altered or lost”
Claim · model-written summary
Adding drying and ether extraction made the assay satisfactory for field-grown sorghum leaves.
- Treatment
- tissue drying and ether extraction stepse0
- Outcome
- satisfactory for use with field‐grown sorghum leavese0
- Comparator
- designed for use with sorghumse edlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inclusion of tissue drying and ether extraction steps in the spectrophotometric assay made this procedure, which was designed for use with sorghumse edlings, satisfactory for use with field‐grown sorghum leaves.”
The same questions in other papers
processing · yield · SorghumPositive and negative results
7 papers in the same direction as this paper · 2 in the opposite direction · 2 reporting no difference from an alternative
- negative
- no effect
- no effect
8 papers more in this group
See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.