Abstract
SUMMARY The flax rust resistance gene L, a nucleotide binding site, leucine-rich repeat (NBS-LRR) class of plant resistance gene, has 12 characterized alleles with different gene-for-gene resistance specificities. Here the specificities of presumptive L1, L5, L8 and L11 genomic clones are confirmed by transgenic expression. L6 and L11 differ by 33 amino acids, 32 in the LRR region and one in the C-terminal non-LRR region, and recognize unrelated avirulence proteins, AvrL567 and AvrL11, respectively. To analyse the specificity differences, 13 L6L11 recombinant genes were constructed in vitro and tested in transgenic flax for resistance to F2 progeny of rust strain CH5, in which the unlinked avirulence genes AvrL567 and AvrL11 segregate. The data show that the single C-terminal non-LRR region polymorphism is not involved in L6-L11 specificity differences, that polymorphisms necessary for specificity are spread throughout the LRR region and that some polymorphisms essential for L11 are not essential for L6. Seven 'null' recombinants expressed no resistance when tested with CH5-derived rusts. These were tested for new resistance specificities by inoculation with a strain of rust, Bs-1, which is distantly related to CH5 and which potentially carries a different range of avirulence specificities. The 'null' recombinant L6L11RV, which differs from L6 and L11 by its susceptibility to CH5, was resistant to strain Bs-1. The specificity difference is due to a reduction in the number of AvrL567 variants recognized by L6L11RV compared with L6 and not due to recognition of an unrelated Avr gene product in strain Bs-1.
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Claims 9
Claim · model-written summary
L6 and L11 differ by 33 amino acids in flax.
- Taxon
- flax
Linum usitatissimumt0 - Gene
- L6 and L11e2
- Outcome
- differ by 33 amino acidse2
- Value
- 33 amino acidse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“L6 and L11 differ by 33 amino acids, 32 in the LRR region and one in the C-terminal non-LRR region”
t0“specificity in flax”
Claim · model-written summary
Presumptive L1, L5, L8 and L11 genomic clones have confirmed resistance specificities by transgenic expression.
- Taxon
- flaxLinum usitatissimumt0
- Gene
- L1, L5, L8 and L11e1
- Outcome
- confirmed by transgenic expressione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the specificities of presumptive L1, L5, L8 and L11 genomic clones are confirmed by transgenic expression”
t0“specificity in flax”
Claim · model-written summary
The single C-terminal non-LRR region polymorphism is not involved in L6-L11 specificity differences.
- Taxon
- flaxLinum usitatissimumt0
- Gene
- L6-L11e1
- Outcome
- L6-L11 specificity differencese1
- Qualifier
- single C-terminal non-LRR region polymorphisme1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The data show that the single C-terminal non-LRR region polymorphism is not involved in L6-L11 specificity differences”
t0“specificity in flax”
Claim · model-written summary
Polymorphisms necessary for L6-L11 specificity are spread throughout the LRR region.
- Taxon
- flaxLinum usitatissimumt0
- Outcome
- polymorphisms necessary for specificitye1
- Qualifier
- spread throughout the LRR regione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“polymorphisms necessary for specificity are spread throughout the LRR region”
t0“specificity in flax”
Claim · model-written summary
Some polymorphisms essential for L11 are not essential for L6.
- Taxon
- flaxLinum usitatissimumt0
- Gene
- L11e1
- Outcome
- polymorphisms essential for L11e1
- Qualifier
- not essential for L6e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“some polymorphisms essential for L11 are not essential for L6”
t0“specificity in flax”
Claim · model-written summary
Seven null recombinants expressed no resistance when tested with CH5-derived rusts.
- Taxon
- flaxLinum usitatissimumt0
- Interactor
- CH5-derived rustse1pathogen
- Outcome
- expressed no resistancee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Seven 'null' recombinants expressed no resistance when tested with CH5-derived rusts”
t0“specificity in flax”
Claim · model-written summary
The null recombinant L6L11RV was resistant to rust strain Bs-1.
- Taxon
- flaxLinum usitatissimumt0
- Interactor
- rust, Bs-1e2pathogen
- Gene
- L6L11RVe1
- Outcome
- resistant to strain Bs-1e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The 'null' recombinant L6L11RV, which differs from L6 and L11 by its susceptibility to CH5, was resistant to strain Bs-1”
e2“inoculation with a strain of rust, Bs-1, which is distantly related to CH5”
t0“specificity in flax”
Claim · model-written summary
L6L11RV recognizes a reduced number of AvrL567 variants compared with L6.
- Taxon
- flaxLinum usitatissimumt0
- Gene
- L6L11RVe1
- Outcome
- number of AvrL567 variants recognizede1
- Direction
- reductione1
- Comparator
- L6e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The specificity difference is due to a reduction in the number of AvrL567 variants recognized by L6L11RV compared with L6”
t0“specificity in flax”
Claim · model-written summary
The L6L11RV specificity difference is not due to recognition of an unrelated Avr gene product in strain Bs-1.
- Taxon
- flaxLinum usitatissimumt0
- Outcome
- recognition of an unrelated Avr gene producte1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“not due to recognition of an unrelated Avr gene product in strain Bs-1”
t0“specificity in flax”