Abstract
A virus with flexuous particles, previously designated chilli veinal mottle virus (CVMV)‐CM1, which causes dark green spot, green vein‐banding and leaf distortion on peppers in Thailand, was isolated and characterized. The host range of this virus was restricted to the Solanaceae. The mean particle length in crude sap was 765 nm and the mean particle width was 13 nm. The virus was purified from pepper or Nicotiana glutinosa by extraction in phosphate buffer, followed by chloroform clarification and precipitation with PEG. The partially purified virus was further purified by sucrose density gradient centrifugation. This protocol consistently yielded 8–19mg virus/kg infected leaves. The purified virus had a UV absorption spectrum typical of nucleoprotein, with a minimum at 246 nm, a maximum at 260 nm, and a slight shoulder at 290 nm. The A 260 /A 280 ratio was in the range 1·20–1·28, and the A max /A min ratio was in the range 1·11–1·27; the buoyant density in CsCl was 1·328g/cm 3 Virions contained a single‐stranded RNA of c. 10kb and a single major protein of molecular weight 34000. The virus induced cytoplasmic inclusions in infected cells, which were observed as pinwheels, scrolls and laminated aggregates. It was not serologically related to potato virus Y (PVY, PVY‐O, PVY‐T), pepper mottle virus (PepMoV), pepper veinal mottle virus (PVMV), tobacco etch virus (TEV), papaya ringspot virus (PRSV), or peanut stripe virus (PStV), but is related to chilli veinal mottle virus occurring in Malaysia. On the basis of particle morphology, physico‐chemical and biological properties and cytopathology, it is considered to be a potyvirus. As this virus was serologically distinct from well‐known potyviruses, it is thought to be a new pepper potyvirus found in Thailand. Since the virus induces a typical dark green spotting, patching and banding along the vein in established infected peppers, the new and more descriptive name ‘chilli vein‐banding mottle virus’(CVbMV) is proposed.
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Claims 12
Claim · model-written summary
The host range of the pepper virus is restricted to the Solanaceae.
- Interactor
- viruse1pathogen
- Outcome
- host rangee1
- Qualifier
- restricted to the Solanaceaee1
- Setting
- Thailande0
CapsicumOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“on peppers in Thailand”
e1“The host range of this virus was restricted to the Solanaceae”
Claim · model-written summary
The mean particle length of the virus in crude sap is 765 nm.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“on peppers in Thailand”
e2“The mean particle length in crude sap was 765 nm”
Claim · model-written summary
The mean particle width of the virus is 13 nm.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“on peppers in Thailand”
e2“the mean particle width was 13 nm”
Claim · model-written summary
The purification protocol consistently yielded 8–19 mg virus per kg of infected leaves.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“on peppers in Thailand”
e3“consistently yielded 8–19mg virus/kg infected leaves”
Claim · model-written summary
The A260/A280 ratio of the purified virus is 1.20–1.28.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“on peppers in Thailand”
e4“The A 260 /A 280 ratio was in the range 1·20–1·28”
Claim · model-written summary
The virion RNA is single-stranded and about 10 kb long.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“on peppers in Thailand”
e5“RNA of c. 10kb”
Claim · model-written summary
The virus has a single major protein of molecular weight 34000.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“on peppers in Thailand”
e5“a single major protein of molecular weight 34000”
Claim · model-written summary
The virus induces cytoplasmic inclusions in infected pepper cells, observed as pinwheels, scrolls and laminated aggregates.
- Interactor
- viruse6pathogen
- Outcome
- cytoplasmic inclusionse6
- Qualifier
- observed as pinwheels, scrolls and laminated aggregatese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“on peppers in Thailand”
e6“The virus induced cytoplasmic inclusions in infected cells, which were observed as pinwheels, scrolls and laminated aggregates”
Claim · model-written summary
The pepper virus is not serologically related to the listed potyviruses and viruses.
- Outcome
- serologically relatede7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“on peppers in Thailand”
e7“It was not serologically related to”
Claim · model-written summary
The pepper virus is related to chilli veinal mottle virus occurring in Malaysia.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“on peppers in Thailand”
e7“but is related to chilli veinal mottle virus occurring in Malaysia”
Claim · model-written summary
On morphological, physicochemical, biological and cytopathological grounds, the pepper virus is considered a potyvirus.
- Outcome
- potyviruse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“on peppers in Thailand”
e1“it is considered to be a potyvirus”
Claim · model-written summary
The pepper virus is serologically distinct from well-known potyviruses and is thought to be a new pepper potyvirus in Thailand.
- Outcome
- serologically distincte1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“on peppers in Thailand”
e1“As this virus was serologically distinct from well”