Abstract
Transgenic apple shoots were prepared from leaf disks by using Agrobacterium tumefaciens carrying the kanamycin (KM) resistance gene and antisense polyphenol oxidase (PPO) DNA. Four transgenic apple lines that grew on the medium containing 50 μg/mL KM were obtained. They contained the KM resistance gene and grew stably on the medium for >3 years. Two transgenic shoot lines containing antisense PPO DNA in which PPO activity was repressed showed a lower browning potential than a control shoot. Keywords: Apple (Malus × domestica); enzymatic browning; polyphenol oxidase; antisense; transgenic plant; Agrobacterium transformation
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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 4
Claim · model-written summary
Four transgenic apple lines grew on kanamycin medium.
- Taxon
- apple
Malus domesticae0 - Material
- transgenic apple linese0
- Outcome
- grew on the mediume0
- Value
- Foure0
- Qualifier
- 50 μg/mL KMe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Four transgenic apple lines that grew on the medium containing 50 μg/mL KM were obtained”
Claim · model-written summary
The lines contained the kanamycin resistance gene and remained stable for over three years.
- Taxon
- appleMalus domesticat0
- Gene
- KM resistance genee0
- Outcome
- grew stably on the mediume0
- Value
- >3 yearse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They contained the KM resistance gene and grew stably on the medium for >3 years”
t0“Apple”
t1“Apple”
Claim · model-written summary
Two antisense PPO lines had repressed PPO activity.
- Taxon
- appleMalus domesticat0
- Gene
- PPOe0
- Outcome
- PPO activitye0
- Direction
- repressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two transgenic shoot lines containing antisense PPO DNA in which PPO activity was repressed”
t0“Apple”
t1“Apple”
Claim · model-written summary
Antisense PPO shoots browned less than control shoots.
- Taxon
- appleMalus domesticat0
- Treatment
- antisense PPO DNAe0
- Outcome
- browning potentiale0
- Direction
- lowere0
- Comparator
- control shoote0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two transgenic shoot lines containing antisense PPO DNA in which PPO activity was repressed showed a lower browning potential than a control shoot”
t0“Apple”
t1“Apple”
The same questions in other papers
loss of function · disease · Malus domestica (apple)Positive and negative results
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See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.