Abstract
In vitro grown shoot tips of cherry plum (Prunus cerasifera Ehrh.) and blackberry ?Cacanska Bestrna? (Rubus fruticosus L.) were tested for regrowth after cryopreservation using encapsulation dehydration method. Apical, 2-3 mm long shoot tips, were encapsulated in alginate beads composed of 3, 5 and 10% (w/v) alginic acid sodium salt in calcium-free liquid Murashige and Skoog (MS) medium containing 1.0 mg l-1 benzyladenine, 0.1 mg l-1 indole-3-butyric acid and 0.1 mg l-1 gibberellic acid. Polymerization was done in liquid MS medium with 100 mM CaCl2 for 30 min at room temperature. Encapsulated shoot tips were pre-treated in liquid MS medium with 0.75 or 1 M sucrose for 24 h in growth room and dehydrated for 4 and 8 h (29% and 20% moisture content respectively) before rapid immersion in liquid nitrogen. Upon thawing which involved placing the cryovials in the air current of the laminar airflow cabinet for 2 min, the beads were directly transferred to regrowth medium. In cherry plum, osmotic dehydration in 0.75 M sucrose followed by 8-hour desiccation gave the highest regrowth (60%) of explants encapsulated in 3% and 5% alginate beads. However, in comparison with cherry plum, blackberry displayed significantly lower capacity for regrowth after cryopreservation under described experimental conditions. In this genotype, osmotic dehydration in 1 M sucrose followed by 8-hour desiccation resulted in the highest regrowth (16.7%) of explants encapsulated in 5% alginate beads. Cryopreserved shoot tips of both genotypes multiplied in the three subcultures had normal morphology and similar multiplication capacity in comparison with non-cryopreserved shoots.
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Claims 5
Claim · model-written summary
In cherry plum, osmotic dehydration in 0.75 M sucrose followed by 8-hour desiccation gave the highest regrowth (60%).
- Taxon
- cherry plum
Prunus cerasiferae0 - Treatment
- osmotic dehydration in 0.75 M sucrose followed by 8-hour desiccatione1
- Outcome
- regrowthe1
- Direction
- higheste1
- Value
- 60%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro grown shoot tips of cherry plum (Prunus cerasifera Ehrh.) and blackberry”
e1“In cherry plum, osmotic dehydration in 0.75 M sucrose followed by 8-hour desiccation gave the highest regrowth (60%) of explants encapsulated in 3% and 5% alginate beads”
Claim · model-written summary
Blackberry had significantly lower capacity for regrowth after cryopreservation than cherry plum.
RubusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro grown shoot tips of cherry plum (Prunus cerasifera Ehrh.) and blackberry”
e2“However, in comparison with cherry plum, blackberry displayed significantly lower capacity for regrowth after cryopreservation under described experimental conditions”
Claim · model-written summary
In blackberry, osmotic dehydration in 1 M sucrose followed by 8-hour desiccation gave the highest regrowth (16.7%).
- Treatment
- osmotic dehydration in 1 M sucrose followed by 8-hour desiccatione3
- Outcome
- regrowthe3
- Direction
- higheste3
- Value
- 16.7%e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro grown shoot tips of cherry plum (Prunus cerasifera Ehrh.) and blackberry”
e3“In this genotype, osmotic dehydration in 1 M sucrose followed by 8-hour desiccation resulted in the highest regrowth (16.7%) of explants encapsulated in 5% alginate beads”
Claim · model-written summary
Cryopreserved cherry plum shoot tips had normal morphology and similar multiplication capacity to non-cryopreserved shoots over three subcultures.
- Taxon
- cherry plumPrunus cerasiferae0
- Treatment
- Cryopreservede4
- Outcome
- multiplication capacitye4
- Direction
- similare4
- Qualifier
- normal morphologye4
- Comparator
- non-cryopreserved shootse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro grown shoot tips of cherry plum (Prunus cerasifera Ehrh.) and blackberry”
e4“Cryopreserved shoot tips of both genotypes multiplied in the three subcultures had normal morphology and similar multiplication capacity in comparison with non-cryopreserved shoots”
Claim · model-written summary
Cryopreserved blackberry shoot tips had normal morphology and similar multiplication capacity to non-cryopreserved shoots over three subcultures.
- Treatment
- Cryopreservede4
- Outcome
- multiplication capacitye4
- Direction
- similare4
- Qualifier
- normal morphologye4
- Comparator
- non-cryopreserved shootse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro grown shoot tips of cherry plum (Prunus cerasifera Ehrh.) and blackberry”
e4“Cryopreserved shoot tips of both genotypes multiplied in the three subcultures had normal morphology and similar multiplication capacity in comparison with non-cryopreserved shoots”
The same questions in other papers
sucrose · growth · Rubus (bramble)Results agree
2 papers in the same direction as this paper
- positive
- positive
drought · growth · Prunus cerasifera (cherry plum)Positive and negative results
1 in the opposite direction
- negative
in vitro · pest · Rubus (bramble)Positive and negative results
1 in the opposite direction
- positive
drought · growth · Rubus (bramble)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.