Abstract
The rate of indole-3-butyric acid (IBA) synthesis in maize seedlings is dependent on the culture conditions of the plants. When the seedlings were grown on filter paper soaked with different amounts of water, the activity of IBA synthetase differed strongly. High amounts of water (150 and 200 ml per bowl) inhibited IBA synthesis completely in vitro, whereas 30 and 50 ml water per bowl increased the activity dramatically. Under conditions where IBA synthetase was inhibited (150 ml H2O), an increase of enzyme activity was observed when abscisic acid (ABA) was exogenously added in concentrations between 5×10−4 to 5×10−7 M. Under ‘drought’ conditions (50 ml H2O per bowl) the same ABA concentrations were inhibitory. Jasmonic acid and salicylic acid also enhanced IBA synthetase activity to some extent, whereas indole-3-acetic acid (IAA) and kinetin had no effect. Activity could also be enhanced by osmotic stress (NaCI and sorbitol), but not under temperature stress. In accompanying investigations the endogenous contents of IAA, IBA, and ABA under the different culture conditions have been determined as well as the energy charge of the seedlings. Similar observations have been made with Amaranthus, wheat and pea seedlings
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Claims 14
Claim · model-written summary
High water amounts completely inhibited in vitro IBA synthesis.
- Treatment
- High amounts of water (150 and 200 ml per bowl)e0
- Gene
- IBA synthetasee2
- Outcome
- IBA synthesise0
- Direction
- inhibitede0
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High amounts of water (150 and 200 ml per bowl) inhibited IBA synthesis completely in vitro”
e1“in maize seedlings”
e2“activity of IBA synthetase differed strongly”
Claim · model-written summary
Lower water amounts dramatically increased IBA synthetase activity.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“30 and 50 ml water per bowl increased the activity dramatically.”
e1“in maize seedlings”
e2“activity of IBA synthetase differed strongly”
Claim · model-written summary
Exogenous ABA increased enzyme activity under the 150 ml water condition.
- Treatment
- abscisic acid (ABA) was exogenously added in concentrations between 5×10−4 to 5×10−7 Me0
- Gene
- IBA synthetasee0
- Outcome
- enzyme activitye0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under conditions where IBA synthetase was inhibited (150 ml H2O), an increase of enzyme activity was observed when abscisic acid (ABA) was exogenously added in concentrations between 5×10−4 to 5×10−7 M.”
e1“in maize seedlings”
Claim · model-written summary
The same ABA concentrations inhibited enzyme activity under drought conditions.
- Treatment
- ABA concentrationse0
- Outcome
- activity of IBA synthetasee2
- Direction
- inhibitorye0
- Qualifier
- ‘drought’ conditions (50 ml H2O per bowl)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under ‘drought’ conditions (50 ml H2O per bowl) the same ABA concentrations were inhibitory.”
e1“in maize seedlings”
e2“activity of IBA synthetase differed strongly”
Claim · model-written summary
Jasmonic acid enhanced IBA synthetase activity.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Jasmonic acid and salicylic acid also enhanced IBA synthetase activity to some extent”
e1“in maize seedlings”
Claim · model-written summary
Salicylic acid enhanced IBA synthetase activity.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Jasmonic acid and salicylic acid also enhanced IBA synthetase activity to some extent”
e1“in maize seedlings”
Claim · model-written summary
IAA had no effect on IBA synthetase activity.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“indole-3-acetic acid (IAA) and kinetin had no effect.”
e1“in maize seedlings”
e2“IBA synthetase activity to some extent”
Claim · model-written summary
Kinetin had no effect on IBA synthetase activity.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“indole-3-acetic acid (IAA) and kinetin had no effect.”
e1“in maize seedlings”
e2“IBA synthetase activity to some extent”
Claim · model-written summary
Osmotic stress from NaCI enhanced enzyme activity.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Activity could also be enhanced by osmotic stress (NaCI and sorbitol)”
e1“in maize seedlings”
e2“activity of IBA synthetase differed strongly”
Claim · model-written summary
Osmotic stress from sorbitol enhanced enzyme activity.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Activity could also be enhanced by osmotic stress (NaCI and sorbitol)”
e1“in maize seedlings”
e2“activity of IBA synthetase differed strongly”
Claim · model-written summary
Temperature stress did not enhance enzyme activity.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not under temperature stress.”
e1“in maize seedlings”
e2“Activity could also be enhanced by osmotic stress”
Claim · model-written summary
Similar observations were reported in Amaranthus seedlings.
- Taxon
- Amaranthus
_with_green_foliage%2C_photographed_in_Tamil_Nadu%2C_India.jpg?width=60)
Amaranthuse0 - Outcome
- Similar observationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_with_green_foliage%2C_photographed_in_Tamil_Nadu%2C_India.jpg?width=500)
Verbatim
e0“Similar observations have been made with Amaranthus, wheat and pea seedlings”
Claim · model-written summary
Similar observations were reported in wheat seedlings.
- Taxon
- wheat
Triticum aestivume0 - Outcome
- Similar observationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Similar observations have been made with Amaranthus, wheat and pea seedlings”
Claim · model-written summary
Similar observations were reported in pea seedlings.
- Taxon
- pea
Lathyrus oleraceuse0 - Outcome
- Similar observationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Similar observations have been made with Amaranthus, wheat and pea seedlings”
The same questions in other papers
(+)-abscisic acid · molecular: metabolites · Zea mays (maize)Positive and negative results
9 papers in the same direction as this paper · 9 in the opposite direction
- positive
- positive
- positive
15 papers more in this group
See all 19 papers →indole-3-acetic acid · physiology: enzyme activity · Zea mays (maize)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.