Paper · 1980 · Annales Botanici Fennici

Aseptic germination of seeds of Northern Terrestrial orchids

Ben R. Lindén

8
claims
16
verbatim quotes
36
citations
2
research areas
—
Not open access

Abstract

Aseptic germination of 27 North European and 2 North American orchids was tested on ZAK medium, which contains coconut liquid and sucrose, CHANG medium, based on fish protein hydrolysate, and two mineral media: FAST's medium for Cypripedium and Burgeffs Eg 1. The two latter media gave good germination and growth, Egl proving better for tuberous species. Of the 29 taxa tested, 25 germinated, the four nongerminating species belonging to the rhizomatous genera Cephalanthera and Epipactis. Immature seeds, when used, gave better germination than mature dried seeds. The time needed for germination to start, 2—10 weeks, was clearly species specific. Cold treatment was not required even in the case of such boreal or alpine species as Calypso bulbosa, Cypripedium passerinum and Chamorchis alpina. The effect of disinfection and the significance of the stage of maturation of the seed are discussed. The extremely juvenile condition of the embryo at seed capsule dehiscence and the heterotrophic stage preceding emergence above the ground may partly explain why the germination behaviour of a species does not change with the latitude or environmental conditions.

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Claims 8

Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

FAST and Burgeffs mineral media gave good germination and growth.

Taxon
orchidse2
Treatment
FAST's medium for Cypripedium and Burgeffs Eg 1e1
Outcome
good germination and growthe0

Not reportedComparatorDirection

Verbatim

e0

“The two latter media gave good germination and growth”

✓ located verbatim in the source · abstract, characters 260–313 · show in text ↑
e1

“FAST's medium for Cypripedium and Burgeffs Eg 1”

✓ located verbatim in the source · abstract, characters 211–258 · show in text ↑
e2

“orchids”

✓ located verbatim in the source · abstract, characters 62–69 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Eg 1 performed better for tuberous species.

Taxon
orchidse2
Treatment
Burgeffs Eg 1e1
Outcome
better for tuberous speciese0
Direction
bettere0

Not reportedComparator

Verbatim

e0

“Egl proving better for tuberous species”

✓ located verbatim in the source · abstract, characters 315–354 · show in text ↑
e1

“Burgeffs Eg 1”

✓ located verbatim in the source · abstract, characters 245–258 · show in text ↑
e2

“orchids”

✓ located verbatim in the source · abstract, characters 62–69 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Twenty-five of 29 orchid taxa germinated.

Taxon
orchidse1
Outcome
germinatede0
Value
25e0

Verbatim

e0

“Of the 29 taxa tested, 25 germinated”

✓ located verbatim in the source · abstract, characters 356–392 · show in text ↑
e1

“orchids”

✓ located verbatim in the source · abstract, characters 62–69 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The four nongerminating species belonged to Cephalanthera and Epipactis.

Taxon
Cephalanthera and EpipactisEpipactisCephalantherae0
Outcome
nongerminating speciese0
Value
foure0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Epipactis
Epipactis · Taxon · picture of the genus (one of its species)BerndH · CC BY-SA 3.0 · Wikimedia Commons ↗
Cephalanthera
Cephalanthera · Taxon · picture of the genus (one of its species)Bernd Haynold · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the four nongerminating species belonging to the rhizomatous genera Cephalanthera and Epipactis”

✓ located verbatim in the source · abstract, characters 394–489 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Immature seeds germinated better than mature dried seeds.

Taxon
orchidse1
Treatment
Immature seedse0
Outcome
germinatione0
Direction
bettere0
Comparator
mature dried seedse0

Verbatim

e0

“Immature seeds, when used, gave better germination than mature dried seeds”

✓ located verbatim in the source · abstract, characters 491–565 · show in text ↑
e1

“orchids”

✓ located verbatim in the source · abstract, characters 62–69 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Germination onset took 2—10 weeks and varied by species.

Taxon
orchidse1
Outcome
time needed for germination to starte0
Value
2—10 weekse0

Verbatim

e0

“The time needed for germination to start, 2—10 weeks, was clearly species specific”

✓ located verbatim in the source · abstract, characters 567–649 · show in text ↑
e1

“orchids”

✓ located verbatim in the source · abstract, characters 62–69 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Cold treatment was not required for the named boreal or alpine species.

Taxon
Calypso bulbosa, Cypripedium passerinum and Chamorchis alpinaCypripedium passerinumCalypso bulbosaChamorchis alpinae0
Treatment
Cold treatmente0
Outcome
not requirede0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cypripedium passerinum
Cypripedium passerinum · TaxonJason Hollinger · CC BY 2.0 · Wikimedia Commons ↗
Calypso bulbosa
Calypso bulbosa · Taxon · picture from its Wikimedia Commons categoryNPS Staff · Public domain · Wikimedia Commons ↗
Chamorchis alpina
Chamorchis alpina · TaxonThierry Arbault · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Cold treatment was not required even in the case of such boreal or alpine species as Calypso bulbosa, Cypripedium passerinum and Chamorchis alpina”

✓ located verbatim in the source · abstract, characters 651–797 · show in text ↑
Mechanismhypothesisoriginal resultmeasured

Claim · model-written summary

Juvenile embryos and a heterotrophic stage may explain latitude-independent germination behavior.

Taxon
orchidse1
Organ / stage
embryoe0
Outcome
germination behaviour of a species does not change with the latitude or environmental conditionse0
Qualifier
heterotrophic stagee0

Verbatim

e0

“The extremely juvenile condition of the embryo at seed capsule dehiscence and the heterotrophic stage preceding emergence above the ground may partly explain why the germination behaviour of a species does not change with the latitude or environmental conditions”

✓ located verbatim in the source · abstract, characters 901–1163 · show in text ↑
e1

“orchids”

✓ located verbatim in the source · abstract, characters 62–69 · show in text ↑