Abstract
A 3.9-kb genomic DNA fragment from the cucurbit pathogen Fusarium solani f. sp. cucurbitae race 2 was cloned. Sequence analysis revealed an open reading frame of 690 nucleotides interrupted by a single 51-bp intron. The nucleotide and predicted amino acid sequences showed 92 and 98% identity, respectively, to those of the cutA gene of the pea pathogen F. solani f. sp. pisi. A gene replacement vector was constructed and used to generate cutA- mutants that were detected with a polymerase chain reaction (PCR) assay. Seventy-one cutA- mutants were identified among the 416 transformants screened. Vector integration was assessed by Southern analysis in 23 of these mutants. PCR and Southern analysis data showed the level of homologous integration was 14%. Disruption of the cutA locus in mutants was confirmed by RNA gel blot hybridization. Neither virulence on Cucurbita maxima cv. Delica at any of six different inoculum concentrations, nor pathogenicity on intact fruit of four different species or cultivars of cucurbit or hypocotyl tissue of C. maxima cv. Crown, was found to be affected by disruption of the cutA gene.
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Claims 3
Claim · model-written summary
Disruption of cutA did not affect virulence on Cucurbita maxima cv. Delica across six inoculum concentrations.
- Taxon
- Cucurbita maxima
Cucurbita maximae0 - Material
- cv. Delicae0
- Treatment
- disruption of the cutA genee0
- Gene
- cutAe0
- Outcome
- virulence on Cucurbita maxima cv. Delicae0
- Direction
- Neithere0
- Qualifier
- six different inoculum concentrationse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Neither virulence on Cucurbita maxima cv. Delica at any of six different inoculum concentrations, nor pathogenicity on intact fruit of four different species or cultivars of cucurbit or hypocotyl tissue of C. maxima cv. Crown, was found to be affected by disruption of the cutA gene.”
Claim · model-written summary
Disruption of cutA did not affect pathogenicity on intact cucurbit fruit.
- Taxon
- cucurbite0
- Treatment
- disruption of the cutA genee0
- Gene
- cutAe0
- Outcome
- pathogenicity on intact fruite0
- Direction
- was found to be affectede0
- Qualifier
- four different species or cultivars of cucurbite0
Not reportedComparator
Verbatim
e0“Neither virulence on Cucurbita maxima cv. Delica at any of six different inoculum concentrations, nor pathogenicity on intact fruit of four different species or cultivars of cucurbit or hypocotyl tissue of C. maxima cv. Crown, was found to be affected by disruption of the cutA gene.”
Claim · model-written summary
Disruption of cutA did not affect pathogenicity on C. maxima cv. Crown hypocotyls.
- Taxon
- C. maximaCucurbita maximae0
- Material
- cv. Crowne0
- Treatment
- disruption of the cutA genee0
- Gene
- cutAe0
- Outcome
- pathogenicity on intact fruit of four different species or cultivars of cucurbit or hypocotyl tissuee0
- Direction
- Neithere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Neither virulence on Cucurbita maxima cv. Delica at any of six different inoculum concentrations, nor pathogenicity on intact fruit of four different species or cultivars of cucurbit or hypocotyl tissue of C. maxima cv. Crown, was found to be affected by disruption of the cutA gene.”