Abstract
Deoxyhypusine synthase (DHS; EC 2.5.1.46) mediates the first of two enzymatic reactions that convert inactive eukaryotic translation initiation factor‐5A (eIF‐5A) to an activated form, thought to facilitate translation. A full‐length cDNA clone encoding canola ( Brassica napus cv. Westar) DHS was isolated from a cDNA‐expression library prepared from senescing leaves. Transgenic canola lines with suppressed DHS expression were obtained by introducing a transgene expressing antisense 3′‐UTR canola DHS cDNA under the regulation of the constitutive cauliflower mosaic virus 35S (CaMV‐35S) promoter. Transformed seed was obtained by vacuum infiltration of canola inflorescences using the protocol developed for Arabidopsis with modifications. The resultant transgenic plants had reduced levels of leaf DHS protein and exhibited delayed natural leaf senescence. Suppression of DHS also increased leaf size by 1.5‐ to two‐fold and resulted in increases in seed yield of up to 65%. Moreover, the enhanced performance of transgenic plants reflected increased tolerance to chronic sublethal stress. When wild‐type and transgenic plants were grown in 6‐inch pots, the increase in seed yield accruing from suppression of DHS was approximately 4.5‐fold greater than when the plants were grown in 12‐inch pots. Thus, suppression of DHS appears to ameliorate the effects of sublethal stress engendered by growth in small containers.
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Claims 7
Claim · model-written summary
Transgenic canola plants with suppressed DHS expression had reduced levels of leaf DHS protein.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse3 - Material
- Westare3
- Organ / stage
- leafe1
- Treatment
- suppressed DHS expressione0
- Gene
- DHSe1
- Outcome
- levels of leaf DHS proteine1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“Transgenic canola lines with suppressed DHS expression”
e1“The resultant transgenic plants had reduced levels of leaf DHS protein and exhibited delayed natural leaf senescence”
e3“Brassica napus cv. Westar”
Claim · model-written summary
Transgenic canola plants with suppressed DHS expression showed delayed natural leaf senescence.
- Taxon
- Brassica napusBrassica napuse3
- Organ / stage
- leafe1
- Treatment
- suppressed DHS expressione0
- Outcome
- natural leaf senescencee1
- Direction
- delayede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic canola lines with suppressed DHS expression”
e1“The resultant transgenic plants had reduced levels of leaf DHS protein and exhibited delayed natural leaf senescence”
e3“Brassica napus cv. Westar”
Claim · model-written summary
Suppression of DHS increased leaf size by 1.5 to two-fold in canola.
- Taxon
- Brassica napusBrassica napuse3
- Organ / stage
- leafe2
- Treatment
- Suppression of DHSe2
- Outcome
- leaf sizee2
- Direction
- increasede2
- Value
- 1.5‐ to two‐folde2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Suppression of DHS also increased leaf size by 1.5‐ to two‐fold and resulted in increases in seed yield of up to 65%”
e3“Brassica napus cv. Westar”
Claim · model-written summary
Suppression of DHS increased seed yield of canola by up to 65%.
- Taxon
- Brassica napusBrassica napuse3
- Treatment
- Suppression of DHSe2
- Outcome
- seed yielde2
- Direction
- increasese2
- Value
- up to 65%e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Suppression of DHS also increased leaf size by 1.5‐ to two‐fold and resulted in increases in seed yield of up to 65%”
e3“Brassica napus cv. Westar”
Claim · model-written summary
The seed yield gain from DHS suppression was approximately 4.5-fold greater in 6-inch pots than in 12-inch pots.
- Taxon
- Brassica napusBrassica napuse3
- Treatment
- suppression of DHSe5
- Outcome
- increase in seed yielde5
- Direction
- greatere5
- Value
- approximately 4.5‐folde5
- Comparator
- 12‐inch potse5
- Setting
- 6‐inch potse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Brassica napus cv. Westar”
e5“When wild‐type and transgenic plants were grown in 6‐inch pots, the increase in seed yield accruing from suppression of DHS was approximately 4.5‐fold greater than when the plants were grown in 12‐inch pots”
Claim · model-written summary
Transgenic canola with suppressed DHS expression showed increased tolerance to chronic sublethal stress.
- Taxon
- Brassica napusBrassica napuse3
- Treatment
- suppressed DHS expressione0
- Outcome
- tolerance to chronic sublethal stresse4
- Direction
- increasede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic canola lines with suppressed DHS expression”
e3“Brassica napus cv. Westar”
e4“Moreover, the enhanced performance of transgenic plants reflected increased tolerance to chronic sublethal stress”
Claim · model-written summary
Suppression of DHS appears to ameliorate the effects of sublethal stress from growth in small containers.
- Taxon
- Brassica napusBrassica napuse3
- Gene
- DHSe6
- Outcome
- effects of sublethal stresse6
- Qualifier
- appears to amelioratee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Brassica napus cv. Westar”
e6“Thus, suppression of DHS appears to ameliorate the effects of sublethal stress engendered by growth in small containers”
The same questions in other papers
loss of function · yield · Brassica napus (oilseed rape)Positive and negative results
1 paper in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- no effect
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See all 6 papers →loss of function · growth · Brassica napus (oilseed rape)Positive and negative results
2 papers in the same direction as this paper · 7 in the opposite direction
- no effect
- negative
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See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.