Abstract
Change in catalase activity was examined in leaves of rice plant exposed to salinity. Depending on the method of preparation of crude protein extract from leaf and the constituents of the assay medium, a significant difference in enzyme activity was recorded. Inclusion of sorbitol or mannitol or sucrose in the extraction and enzyme assay medium enhanced the enzyme activity in salt-stressed samples by nearly 1.5-1.8 fold, compared to the activity found in un- stressed plants, which otherwise showed a 50% declined activity in leaf extract prepared in buffer solution and assayed in a medium depleted of these sugars. In view of the accumulation of osmolytes under saline condition, these observations suggest that the catalase activity is modulated by the osmolytes and maintains a high rate of hydrogen peroxide scavenging property in vivo and serves as the major antioxidant enzyme to scavenge the salt-induced formation of peroxide. Therefore, the salt-stress induced appearance of low activity of the enzyme under normal buffer extraction and assay conditions, as reported in literature may represent an apparent than for its real in vivo activity.
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Claims 7
Claim · model-written summary
Catalase activity differed significantly with extract preparation and assay medium.
- Taxon
- rice
Oryza sativat0 - Organ / stage
- leafe0
- Treatment
- constituents of the assay mediume0
- Outcome
- enzyme activitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Depending on the method of preparation of crude protein extract from leaf and the constituents of the assay medium, a significant difference in enzyme activity was recorded”
t0“rice”
Claim · model-written summary
Sorbitol in extraction and assay medium enhanced catalase activity in salt-stressed samples by nearly 1.5-1.8 fold versus unstressed plants.
- Taxon
- riceOryza sativat0
- Treatment
- sorbitole0
- Outcome
- enzyme activitye0
- Direction
- enhancede0
- Value
- nearly 1.5-1.8 folde0
- Comparator
- un- stressed plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inclusion of sorbitol or mannitol or sucrose in the extraction and enzyme assay medium enhanced the enzyme activity in salt-stressed samples by nearly 1.5-1.8 fold, compared to the activity found in un- stressed plants”
t0“rice”
Claim · model-written summary
Mannitol in extraction and assay medium enhanced catalase activity in salt-stressed samples by nearly 1.5-1.8 fold versus unstressed plants.
- Taxon
- riceOryza sativat0
- Treatment
- mannitole0
- Outcome
- enzyme activitye0
- Direction
- enhancede0
- Value
- nearly 1.5-1.8 folde0
- Comparator
- un- stressed plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inclusion of sorbitol or mannitol or sucrose in the extraction and enzyme assay medium enhanced the enzyme activity in salt-stressed samples by nearly 1.5-1.8 fold, compared to the activity found in un- stressed plants”
t0“rice”
Claim · model-written summary
Sucrose in extraction and assay medium enhanced catalase activity in salt-stressed samples by nearly 1.5-1.8 fold versus unstressed plants.
- Taxon
- riceOryza sativat0
- Treatment
- sucrosee0
- Outcome
- enzyme activitye0
- Direction
- enhancede0
- Value
- nearly 1.5-1.8 folde0
- Comparator
- un- stressed plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inclusion of sorbitol or mannitol or sucrose in the extraction and enzyme assay medium enhanced the enzyme activity in salt-stressed samples by nearly 1.5-1.8 fold, compared to the activity found in un- stressed plants”
t0“rice”
Claim · model-written summary
Without these sugars, salt-stressed leaf extracts showed a 50% decline in catalase activity.
- Taxon
- riceOryza sativat0
- Treatment
- medium depleted of these sugarse0
- Outcome
- activity in leaf extracte0
- Direction
- declinede0
- Value
- 50%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which otherwise showed a 50% declined activity in leaf extract prepared in buffer solution and assayed in a medium depleted of these sugars”
t0“rice”
Claim · model-written summary
The authors suggest osmolytes modulate catalase activity to maintain peroxide scavenging in vivo.
- Taxon
- riceOryza sativat0
- Compound
- osmolytese0
- Outcome
- hydrogen peroxide scavenging property in vivoe0
- Qualifier
- catalase activity is modulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these observations suggest that the catalase activity is modulated by the osmolytes and maintains a high rate of hydrogen peroxide scavenging property in vivo”
t0“rice”
Claim · model-written summary
The authors suggest low catalase activity under normal buffer conditions is apparent rather than its true in vivo activity.
- Taxon
- riceOryza sativat0
- Outcome
- low activity of the enzymee0
- Qualifier
- under normal buffer extraction and assay conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the salt-stress induced appearance of low activity of the enzyme under normal buffer extraction and assay conditions, as reported in literature may represent an apparent than for its real in vivo activity”
t0“rice”
The same questions in other papers
sucrose · soil: biology · Oryza sativa (rice)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.