Abstract
Nitrogen partitioning among proteins in chloroplasts and mitochondria was examined in pea (Pisum sativum L.) and wheat (Triticum aestivum L.) grown hydroponically with different nitrogen concentrations. In pea leaves, chloroplast nitrogen accounted for 75 to 80% of total leaf nitrogen. We routinely found that 8% of total ribulose-1,5-bisphosphate carboxylase/oxygenase adhered to thylakoids during preparation and could be removed with Triton X-100. With this precaution, the ratio of stroma nitrogen increased from 53 to 61% of total leaf nitrogen in response to the nitrogen supply, but thylakoid nitrogen remained almost constant around 20% of total. The changes in the activities of the stromal enzymes and electron transport in response to the nitrogen supply reflected the nitrogen partitioning into stroma and thylakoids. On the other hand, nitrogen partitioning into mitochondria was appreciably smaller than that in chloroplasts, and the ratio of nitrogen allocated to mitochondria decreased with increasing leaf-nitrogen content, ranging from 7 to 4% of total leaf nitrogen. The ratio of mitochondrial respiratory enzyme activities to leaf-nitrogen content also decreased with increasing leaf-nitrogen content. These differences in nitrogen partitioning between chloroplasts and mitochondria were reflected in differences in the rates of photosynthesis and dark respiration in wheat leaves measured with an open gas-exchange system. The response of photosynthesis to nitrogen supply was much greater than that of dark respiration, and the CO(2) compensation point decreased with increasing leaf-nitrogen content.
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Claims 9
Claim · model-written summary
Pea chloroplast nitrogen accounted for 75 to 80% of leaf nitrogen.
- Taxon
- pea
Lathyrus oleraceuse0 - Outcome
- chloroplast nitrogene0
- Value
- 75 to 80% of total leaf nitrogene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In pea leaves, chloroplast nitrogen accounted for 75 to 80% of total leaf nitrogen.”
Claim · model-written summary
Eight percent of Rubisco adhered to thylakoids during preparation.
- Taxon
- peaLathyrus oleraceuse1
- Gene
- ribulose-1,5-bisphosphate carboxylase/oxygenasee0
- Outcome
- adhered to thylakoidse0
- Value
- 8%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“8% of total ribulose-1,5-bisphosphate carboxylase/oxygenase adhered to thylakoids during preparation”
e1“pea (Pisum sativum L.)”
Claim · model-written summary
Stroma nitrogen rose from 53 to 61% with nitrogen supply.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- nitrogen supplye0
- Outcome
- ratio of stroma nitrogene0
- Direction
- increasede0
- Value
- 53 to 61% of total leaf nitrogene0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ratio of stroma nitrogen increased from 53 to 61% of total leaf nitrogen in response to the nitrogen supply”
e1“pea (Pisum sativum L.)”
Claim · model-written summary
Thylakoid nitrogen stayed near 20% of total leaf nitrogen.
- Taxon
- peaLathyrus oleraceuse1
- Outcome
- thylakoid nitrogene0
- Direction
- remained almost constante0
- Value
- 20% of totale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“thylakoid nitrogen remained almost constant around 20% of total”
e1“pea (Pisum sativum L.)”
Claim · model-written summary
Stromal enzyme activities and electron transport reflected nitrogen partitioning.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- nitrogen supplye0
- Outcome
- activities of the stromal enzymes and electron transporte0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The changes in the activities of the stromal enzymes and electron transport in response to the nitrogen supply reflected the nitrogen partitioning into stroma and thylakoids.”
e1“pea (Pisum sativum L.)”
Claim · model-written summary
Mitochondrial nitrogen allocation decreased from 7 to 4% as leaf nitrogen rose.
- Taxon
- peaLathyrus oleraceuse1
- Organ / stage
- leafe0
- Outcome
- ratio of nitrogen allocated to mitochondriae0
- Direction
- decreasede0
- Value
- 7 to 4% of total leaf nitrogene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ratio of nitrogen allocated to mitochondria decreased with increasing leaf-nitrogen content, ranging from 7 to 4% of total leaf nitrogen.”
e1“pea (Pisum sativum L.)”
Claim · model-written summary
Mitochondrial respiratory enzyme activity relative to leaf nitrogen declined.
- Taxon
- peaLathyrus oleraceuse1
- Organ / stage
- leafe0
- Outcome
- ratio of mitochondrial respiratory enzyme activities to leaf-nitrogen contente0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ratio of mitochondrial respiratory enzyme activities to leaf-nitrogen content also decreased with increasing leaf-nitrogen content.”
e1“pea (Pisum sativum L.)”
Claim · model-written summary
Photosynthesis responded more strongly than dark respiration to nitrogen supply.
- Taxon
- wheat
Triticum aestivume1 - Treatment
- nitrogen supplye0
- Outcome
- response of photosynthesise0
- Direction
- much greatere0
- Comparator
- dark respiratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The response of photosynthesis to nitrogen supply was much greater than that of dark respiration”
e1“wheat (Triticum aestivum L.)”
Claim · model-written summary
CO2 compensation point declined as leaf nitrogen increased.
- Taxon
- wheatTriticum aestivume1
- Organ / stage
- leafe0
- Outcome
- CO(2) compensation pointe0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the CO(2) compensation point decreased with increasing leaf-nitrogen content.”
e1“wheat (Triticum aestivum L.)”
The same questions in other papers
nitrogen · physiology: photosynthesis · Triticum aestivum (wheat)Positive and negative results
97 papers in the same direction as this paper · 22 in the opposite direction
- negative
- no effect
- negative
116 papers more in this group
See all 120 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.