Abstract
Abstract The effect of colchicine added to induction medium for the production of fertile doubled haploid plants after in‐vitro anther culture was studied in wheat, Triticum aestivum L. For this, one winter and two spring wheat varieties were used. Anther cultures of the three genotypes were treated with 0.03% colchicine for 3 days at the beginning of microspore induction. Colchicine had no significant effect on anther response and embryoid production of the genotypes examined. However, in the winter wheat genotype ‘Mv Szigma’, colchicine caused a significant reduction in microspore‐derived structures. A significant decrease was also observed in plant regeneration ability of two genotypes (‘Vergina’ and ‘Acheloos’) after colchicine treatment. In addition, a significant reduction of the albinos produced was observed in all genotypes after olchicine treatment. In contrast, the regenerants obtained from the colchicine‐supplemented induction media produced significantly higher percentages of fertile plants in all genotypes. However, the level of fertility, was significantly different among the fertile plants obtained. This, together with the observation that in the case of the winter wheat variety the colchicine treatment resulted in 100% completely fertile plants with a high seed‐setting ability indicate that there is space for further improvement of the method when it is applied to spring cultivars. Finally, the increased number of seeds per 100 plated anthers obtained from all three genotypes after colchicine treatment, clearly demonstrates that the addition of colchicine to induction medium was superior to the conventional anther culture method and it could therefore be introduced into wheat breeding programmes.
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Claims 9
Claim · model-written summary
Colchicine did not significantly affect anther response or embryoid production across the examined genotypes.
- Taxon
- wheat
Triticum aestivume1 - Treatment
- Colchicinee0
- Outcome
- anther response and embryoid productione0
- Direction
- no significant effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Colchicine had no significant effect on anther response and embryoid production of the genotypes examined”
e1“studied in wheat, Triticum aestivum L.”
Claim · model-written summary
Colchicine significantly reduced microspore-derived structures in Mv Szigma.
- Taxon
- wheatTriticum aestivume0
- Material
- Mv Szigmae0
- Treatment
- colchicinee0
- Outcome
- microspore‐derived structurese0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the winter wheat genotype ‘Mv Szigma’, colchicine caused a significant reduction in microspore‐derived structures”
Claim · model-written summary
Colchicine significantly decreased plant regeneration ability in Vergina and Acheloos.
- Taxon
- wheatTriticum aestivume1
- Material
- ‘Vergina’ and ‘Acheloos’e0
- Treatment
- colchicinee0
- Outcome
- plant regeneration abilitye0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A significant decrease was also observed in plant regeneration ability of two genotypes (‘Vergina’ and ‘Acheloos’) after colchicine treatment”
e1“studied in wheat, Triticum aestivum L.”
Claim · model-written summary
Colchicine significantly reduced albino production in all genotypes.
- Taxon
- wheatTriticum aestivume1
- Treatment
- olchicine treatmente0
- Outcome
- albinos producede0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a significant reduction of the albinos produced was observed in all genotypes after olchicine treatment”
e1“studied in wheat, Triticum aestivum L.”
Claim · model-written summary
Regenerants from colchicine-supplemented medium had significantly higher fertile-plant percentages.
- Taxon
- wheatTriticum aestivume1
- Treatment
- colchicine‐supplemented induction mediae0
- Outcome
- percentages of fertile plantse0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the regenerants obtained from the colchicine‐supplemented induction media produced significantly higher percentages of fertile plants in all genotypes”
e1“studied in wheat, Triticum aestivum L.”
Claim · model-written summary
Fertility level differed significantly among fertile plants.
- Taxon
- wheatTriticum aestivume1
- Outcome
- level of fertilitye0
- Direction
- differente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the level of fertility, was significantly different among the fertile plants obtained”
e1“studied in wheat, Triticum aestivum L.”
Claim · model-written summary
Colchicine treatment gave the winter wheat variety 100% completely fertile plants with high seed setting.
- Taxon
- wheatTriticum aestivume1
- Material
- winter wheat varietye0
- Treatment
- colchicine treatmente0
- Outcome
- completely fertile plantse0
- Value
- 100%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the case of the winter wheat variety the colchicine treatment resulted in 100% completely fertile plants with a high seed‐setting ability”
e1“studied in wheat, Triticum aestivum L.”
Claim · model-written summary
Colchicine increased seeds per 100 plated anthers in all three genotypes over conventional culture.
- Taxon
- wheatTriticum aestivume2
- Treatment
- colchicine treatmente0
- Outcome
- number of seeds per 100 plated antherse0
- Direction
- increasede0
- Comparator
- conventional anther culture methode1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased number of seeds per 100 plated anthers obtained from all three genotypes after colchicine treatment”
e1“superior to the conventional anther culture method”
e2“studied in wheat, Triticum aestivum L.”
Claim · model-written summary
The authors recommended colchicine addition to induction medium for wheat breeding programmes.
- Taxon
- wheatTriticum aestivume0
- Outcome
- introduced into wheat breeding programmese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the addition of colchicine to induction medium was superior to the conventional anther culture method and it could therefore be introduced into wheat breeding programmes”
The same questions in other papers
colchicine · growth · Triticum aestivum (wheat)Results differ
3 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- no effect
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See all 9 papers →colchicine · yield · Triticum aestivum (wheat)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.