Abstract
The differential display technique was used to generate cDNA probes in order to identify mRNAs that are up‐regulated during senescence of Arabidopsis leaves. Three mRNAs were examined that had not previously been associated with senescence. The steady‐state levels of these mRNAs are detectable in small amounts in mature green leaves, but increase considerably as chlorophyll levels begin to decline. This relationship to senescence occurs under natural circumstances as well as when senescence is accelerated by leaf detachment in the dark or by addition of 1‐aminocyclopropane‐1‐carboxylic acid (ACC). Retardation of senescence by benzyladenine slows the increase of the mRNAs. One of these mRNAs appears to code for a protein (Sec 13) that may be involved in vesicle formation at the endoplasmic reticulum. Another mRNA codes for a protein with WD‐repeat motif whose function is as yet unidentified, and the third codes for a putative calcium‐dependent protein kinase. A fourth cDNA has also been cloned by subtractive hybridization from senescing Arabidopsis leaves that encodes vacuolar‐processing enzyme ( γ VPE). Incubation of detached leaves in darkness also caused an abrupt elevation in the steady‐state levels of the γVPE , similar to that of the senescing attached leaves. The possible functions of the gene products and their involvement in cellular and biochemical processes during senescence are discussed.
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Claims 9
Claim · model-written summary
Three previously unassociated mRNAs rose as chlorophyll declined during senescence.
- Taxon
- Arabidopsis
Arabidopsise1 - Organ / stage
- mature green leavese0
- Outcome
- steady‐state levels of these mRNAse0
- Direction
- increase considerablye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three mRNAs were examined that had not previously been associated with senescence. The steady‐state levels of these mRNAs are detectable in small amounts in mature green leaves, but increase considerably as chlorophyll levels begin to decline.”
e1“senescence of Arabidopsis leaves.”
Claim · model-written summary
The mRNA rise also occurred with senescence accelerated by leaf detachment in darkness.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- leaf detachment in the darke0
- Outcome
- senescencee0
- Direction
- acceleratede0
- Comparator
- natural circumstancese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This relationship to senescence occurs under natural circumstances as well as when senescence is accelerated by leaf detachment in the dark”
e1“senescence of Arabidopsis leaves.”
Claim · model-written summary
ACC accelerated senescence with the same mRNA relationship.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- addition of 1‐aminocyclopropane‐1‐carboxylic acid (ACC)e0
- Outcome
- senescencee0
- Direction
- acceleratede0
- Comparator
- natural circumstancese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This relationship to senescence occurs under natural circumstances as well as when senescence is accelerated by leaf detachment in the dark or by addition of 1‐aminocyclopropane‐1‐carboxylic acid (ACC).”
e1“senescence of Arabidopsis leaves.”
Claim · model-written summary
Benzyladenine slowed the senescence associated mRNA increase.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- benzyladeninee0
- Outcome
- increase of the mRNAse0
- Direction
- slowse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Retardation of senescence by benzyladenine slows the increase of the mRNAs.”
e1“senescence of Arabidopsis leaves.”
Claim · model-written summary
One senescence associated mRNA apparently encodes Sec 13.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- Sec 13e0
- Outcome
- code for a protein (Sec 13)e0
- Qualifier
- may be involved in vesicle formation at the endoplasmic reticulume0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One of these mRNAs appears to code for a protein (Sec 13) that may be involved in vesicle formation at the endoplasmic reticulum.”
e1“senescence of Arabidopsis leaves.”
Claim · model-written summary
A second mRNA encodes an unidentified WD repeat protein.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- protein with WD‐repeat motife0
- Outcome
- codes for a protein with WD‐repeat motife0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Another mRNA codes for a protein with WD‐repeat motif whose function is as yet unidentified”
e1“senescence of Arabidopsis leaves.”
Claim · model-written summary
A third mRNA encodes a putative calcium dependent protein kinase.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- calcium‐dependent protein kinasee0
- Outcome
- codes for a putative calcium‐dependent protein kinasee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the third codes for a putative calcium‐dependent protein kinase.”
e1“senescence of Arabidopsis leaves.”
Claim · model-written summary
A fourth cDNA from senescing leaves encodes gamma VPE.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- senescing Arabidopsis leavese0
- Gene
- γ VPEe0
- Outcome
- encodes vacuolar‐processing enzymee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A fourth cDNA has also been cloned by subtractive hybridization from senescing Arabidopsis leaves that encodes vacuolar‐processing enzyme ( γ VPE).”
Claim · model-written summary
Dark incubation of detached leaves abruptly raised gamma VPE mRNA.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- Incubation of detached leaves in darknesse0
- Gene
- γVPEe0
- Outcome
- steady‐state levels of the γVPEe0
- Direction
- abrupt elevatione0
- Comparator
- senescing attached leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Incubation of detached leaves in darkness also caused an abrupt elevation in the steady‐state levels of the γVPE , similar to that of the senescing attached leaves.”
e1“senescence of Arabidopsis leaves.”
The same questions in other papers
light · quality: postharvest · ArabidopsisPositive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.