Abstract
The Arabidopsis RTE1 (REVERSION-TO-ETHYLENE SENSITIVITY1) and its tomato ortholog GREEN RIPE (GR) are important in plant ethylene signaling, response and/or fruit ripening. To investigate the potential role of RTE1-like genes in apple (M. ×domestica Borkh.) fruit ripening, two orthologs of RTE1 were identified in the apple genome, designated MdRTE1a and MdRTE1b. Using yeast two-hybrid (Y2H) approach, a cDNA prey library was constructed from a pool of maturing/ripening fruit of ‘Golden Delicious’. Screening the library with MdRTE1a identified two putative NAC (NAM/ATAF1, 2/CUC2) proteins, designated MdNAC1 and MdNAC2, that interacted with MdRTE1a in yeast cells. MdRTE1b, a closely related paralog of MdRTE1a, was also confirmed to be interactive with MdNAC1 and MdNAC2 using the Y2H system. The expression of MdRTE1a and MdNAC1 was high in young fruit but low in more developed and ripening fruit. This pattern of low expression remained largely consistent and was not affected by treatment with 1-methylcyclopropene (1-MCP), an inhibitor of ethylene perception, or ethephon, an ethylene releasing compound. However, the expression of MdRTE1b and MdNAC2 rapidly increased during fruit ripening and was highly inhibited by 1-MCP. These data suggested that MdRTE1a, MdNAC1 and their possible interactions in apple cells may play roles in growth, whereas MdRTE1b, MdNAC2 and their putative interactions are involved in ethylene signaling, response and fruit ripening. The possible interactions between an RTE1-like protein and a NAC protein in plant cells have not been reported thus far. Its implications in ethylene signaling and response are discussed.
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Claims 9
Claim · model-written summary
Two RTE1 orthologs, MdRTE1a and MdRTE1b, were identified in apple.
- Taxon
- apple
Malus domesticae0 - Gene
- MdRTE1a and MdRTE1be0
- Outcome
- two orthologs of RTE1e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two orthologs of RTE1 were identified in the apple genome, designated MdRTE1a and MdRTE1b”
Claim · model-written summary
MdNAC1 and MdNAC2 interacted with MdRTE1a in yeast cells.
- Taxon
- AppleMalus domesticat0
- Gene
- MdNAC1 and MdNAC2e0
- Outcome
- interacted with MdRTE1ae0
- Setting
- yeast cellse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MdRTE1a identified two putative NAC (NAM/ATAF1, 2/CUC2) proteins, designated MdNAC1 and MdNAC2, that interacted with MdRTE1a in yeast cells”
t0“Apple”
Claim · model-written summary
MdRTE1b interacted with MdNAC1 and MdNAC2 in yeast.
- Taxon
- AppleMalus domesticat0
- Gene
- MdRTE1be0
- Outcome
- interactive with MdNAC1 and MdNAC2e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MdRTE1b, a closely related paralog of MdRTE1a, was also confirmed to be interactive with MdNAC1 and MdNAC2 using the Y2H system”
t0“Apple”
Claim · model-written summary
MdRTE1a and MdNAC1 expression was high in young fruit and low in developed and ripening fruit.
- Taxon
- AppleMalus domesticat0
- Organ / stage
- young fruite0
- Gene
- MdRTE1a and MdNAC1e0
- Outcome
- expression of MdRTE1a and MdNAC1e0
- Direction
- high in young fruit but lowe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of MdRTE1a and MdNAC1 was high in young fruit but low in more developed and ripening fruit”
t0“Apple”
Claim · model-written summary
1-MCP and ethephon did not affect the low-expression pattern of MdRTE1a and MdNAC1.
- Taxon
- AppleMalus domesticat0
- Treatment
- 1-methylcyclopropene (1-MCP)e0
- Outcome
- low expressione0
- Direction
- not affectede0
Not reportedGeneComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This pattern of low expression remained largely consistent and was not affected by treatment with 1-methylcyclopropene (1-MCP), an inhibitor of ethylene perception, or ethephon, an ethylene releasing compound”
t0“Apple”
Claim · model-written summary
MdRTE1b and MdNAC2 expression rapidly increased during fruit ripening.
- Taxon
- AppleMalus domesticat0
- Organ / stage
- fruit ripeninge0
- Gene
- MdRTE1b and MdNAC2e0
- Outcome
- expression of MdRTE1b and MdNAC2e0
- Direction
- rapidly increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of MdRTE1b and MdNAC2 rapidly increased during fruit ripening”
t0“Apple”
Claim · model-written summary
1-MCP highly inhibited expression of MdRTE1b and MdNAC2.
- Taxon
- AppleMalus domesticat0
- Treatment
- 1-MCPe0
- Gene
- MdRTE1b and MdNAC2e0
- Outcome
- expression of MdRTE1b and MdNAC2e0
- Direction
- highly inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, the expression of MdRTE1b and MdNAC2 rapidly increased during fruit ripening and was highly inhibited by 1-MCP”
t0“Apple”
Claim · model-written summary
Authors suggest MdRTE1a and MdNAC1 interactions may have roles in growth.
- Taxon
- appleMalus domesticae0
- Gene
- MdRTE1a, MdNAC1e0
- Outcome
- growthe0
- Qualifier
- possible interactionse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MdRTE1a, MdNAC1 and their possible interactions in apple cells may play roles in growth”
Claim · model-written summary
Authors suggest MdRTE1b and MdNAC2 interactions are involved in ethylene signaling, response and ripening.
- Taxon
- AppleMalus domesticat0
- Gene
- MdRTE1b, MdNAC2e0
- Outcome
- ethylene signaling, response and fruit ripeninge0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MdRTE1b, MdNAC2 and their putative interactions are involved in ethylene signaling, response and fruit ripening”
t0“Apple”
The same questions in other papers
1-methylcyclopropene · molecular: expression · Malus domestica (apple)Positive and negative results
3 papers in the same direction as this paper · 6 in the opposite direction
- positive
- positive
- positive
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.