Abstract
1. Treatment of the etiolated maize seedlings with the plant hormone, gibberellic acid results in a significant enhancement of heavy polyribosome formation. 2. This is accompanied by highly increased incorporation of the labelled RNA precursors into RNA engaged in the polyribosomal complex, as well as by an increased rate of protein synthesis in vivo. 3. Determination of the specific radioactivity of particular RNA classes isolated from polyribosomes reveals that gibberellic acid stimulates mostly the synthesis of the rapidly labelled, non-ribosomal RNA fraction. 4. A considerable amount of this rapidly labelled RNA fraction, whose synthesis is preferentially stimulated by exogenous gibberellic acid contains poly(A) sequences, as shown by affinity chromatography on oligo (dT)-cellulose indicating that phytohormone causes an increased transcription of mRNA in etiolated maize seedlings. 5. When [3H]adenosine served as the RNA precursor it was found that the ratio between the heteropolymeric and polyadenylic parts of the poly(A)-RNA chain markedly changed under gibberellin treatment, suggesting that, in addition to an increased rate of mRNA synthesis, the plant hormone also affects the process of post-transcriptional polyadenylation of the newly made mRNA precursors. Possible extension of the polyadenylate segment in the presence of gibberellin may account for a longer functional half-life of the mRNA synthesized in plants treated with the phytohormone, and may explain significantly enhanced heavy polyribosome formation, as well as a higher efficiency of protein synthesis in plants treated with gibberellic acid.
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Claims 8
Claim · model-written summary
Gibberellic acid significantly enhanced heavy polyribosome formation in etiolated maize seedlings.
- Organ / stage
- etiolated maize seedlingse0
- Treatment
- gibberellic acide0
- Outcome
- heavy polyribosome formatione0
- Direction
- enhancemente0
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of the etiolated maize seedlings with the plant hormone, gibberellic acid results in a significant enhancement of heavy polyribosome formation.”
Claim · model-written summary
Gibberellic acid increased labelled RNA precursor incorporation into polyribosomal RNA.
- Treatment
- Gibberellic Acidt0
- Outcome
- incorporation of the labelled RNA precursors into RNA engaged in the polyribosomal complexe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This is accompanied by highly increased incorporation of the labelled RNA precursors into RNA engaged in the polyribosomal complex, as well as by an increased rate of protein synthesis in vivo.”
t0“Plant mRNA Synthesis by Gibberellic Acid”
e1“Treatment of the etiolated maize seedlings”
Claim · model-written summary
Gibberellic acid increased the rate of protein synthesis in vivo.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This is accompanied by highly increased incorporation of the labelled RNA precursors into RNA engaged in the polyribosomal complex, as well as by an increased rate of protein synthesis in vivo.”
t0“Plant mRNA Synthesis by Gibberellic Acid”
e1“Treatment of the etiolated maize seedlings”
Claim · model-written summary
Gibberellic acid preferentially stimulated synthesis of rapidly labelled non-ribosomal RNA.
- Treatment
- gibberellic acide0
- Outcome
- synthesis of the rapidly labelled, non-ribosomal RNA fractione0
- Direction
- stimulatese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gibberellic acid stimulates mostly the synthesis of the rapidly labelled, non-ribosomal RNA fraction.”
t0“Plant mRNA Synthesis by Gibberellic Acid”
e1“Treatment of the etiolated maize seedlings”
Claim · model-written summary
A considerable amount of the rapidly labelled RNA fraction contained poly(A) sequences.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A considerable amount of this rapidly labelled RNA fraction, whose synthesis is preferentially stimulated by exogenous gibberellic acid contains poly(A) sequences”
t0“Plant mRNA Synthesis by Gibberellic Acid”
e1“Treatment of the etiolated maize seedlings”
Claim · model-written summary
Under gibberellin treatment, the ratio between heteropolymeric and polyadenylic parts of poly(A)-RNA changed markedly.
- Treatment
- gibberellin treatmente0
- Outcome
- ratio between the heteropolymeric and polyadenylic parts of the poly(A)-RNA chaine0
- Direction
- changede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ratio between the heteropolymeric and polyadenylic parts of the poly(A)-RNA chain markedly changed under gibberellin treatment”
t0“Plant mRNA Synthesis by Gibberellic Acid”
e1“Treatment of the etiolated maize seedlings”
Claim · model-written summary
The authors infer that gibberellin affects post-transcriptional polyadenylation of newly made mRNA precursors.
- Outcome
- post-transcriptional polyadenylation of the newly made mRNA precursorse0
- Qualifier
- plant hormonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the plant hormone also affects the process of post-transcriptional polyadenylation of the newly made mRNA precursors”
t0“Plant mRNA Synthesis by Gibberellic Acid”
e1“Treatment of the etiolated maize seedlings”
Claim · model-written summary
The authors propose that extension of the polyadenylate segment may lengthen mRNA functional half-life.
- Treatment
- gibberelline0
- Outcome
- functional half-life of the mRNAe0
- Qualifier
- Possible extension of the polyadenylate segmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Possible extension of the polyadenylate segment in the presence of gibberellin may account for a longer functional half-life of the mRNA synthesized in plants treated with the phytohormone”
t0“Plant mRNA Synthesis by Gibberellic Acid”
e1“Treatment of the etiolated maize seedlings”
The same questions in other papers
Gibberellic acid · quality: protein · Zea mays (maize)Positive and negative results
4 papers in the same direction as this paper · 1 reporting no difference from an alternative
- no effect
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.