Abstract
The effect of ethylene treatment on polar indole-3-acetic acid (IAA) transport, net IAA uptake in the presence and absence of N-1-naphthylphthalamic acid (NPA) and [(3)H]NPA binding characteristics was investigated in tissue segments or microsomes isolated from etiolated pea (Pisum sativum L. cv Alaska) epicotyls. Basipetal IAA transport in 5 millimeter segments isolated from ethylene-treated seedlings was inhibited by ethylene in a dose-dependent manner. Threshold, half-maximal and saturating concentrations of ethylene were 0.01, 0.55, 10.0 microliters per liter, respectively. This inhibition became apparent after 6 to 8 hours of ethylene treatment. Transport velocity in both control and ethylene-treated tissues was estimated to be 5 millimeters per hour. Net IAA uptake was stimulated in ethylene-treated tissues and the relative ability of the phytotropin NPA to enhance net IAA uptake was reduced in treated tissues. Specific binding of [(3)H]NPA to microsomes prepared from both control and ethylene-treated tissues was saturable and consistent with the existence of a single class of binding sites with an apparent affinity (K(d)) toward NPA of 8 to 9 nanomolar. The density of these binding sites (per milligram protein) was lower (36% of control) in ethylene-treated tissues. Direct application of ethylene to microsomal preparations isolated from untreated seedlings had no effect on the level of specific [(3)H]NPA binding.
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Claims 10
Claim · model-written summary
Ethylene inhibited basipetal IAA transport in pea segments in a dose-dependent manner.
- Taxon
- Pisum sativum
Lathyrus oleraceuse2 - Material
- cv Alaskae2
- Treatment
- ethylenee1
- Outcome
- Basipetal IAA transporte1
- Direction
- inhibitede1
- Qualifier
- in a dose-dependent mannere1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Basipetal IAA transport in 5 millimeter segments isolated from ethylene-treated seedlings was inhibited by ethylene in a dose-dependent manner”
e2“Pisum sativum L. cv Alaska”
Claim · model-written summary
Threshold, half-maximal and saturating ethylene concentrations were 0.01, 0.55 and 10.0 microliters per liter in pea.
- Taxon
- Pisum sativumLathyrus oleraceuse2
- Compound
- ethylenee3
- Outcome
- Threshold, half-maximal and saturating concentrations of ethylenee3
- Value
- 0.01, 0.55, 10.0 microliters per litere3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Threshold, half-maximal and saturating concentrations of ethylene were 0.01, 0.55, 10.0 microliters per liter”
e2“Pisum sativum L. cv Alaska”
Claim · model-written summary
The ethylene inhibition of IAA transport became apparent after 6 to 8 hours of ethylene treatment in pea.
- Taxon
- Pisum sativumLathyrus oleraceuse2
- Treatment
- ethylene treatmente4
- Outcome
- This inhibition became apparente4
- Value
- 6 to 8 hourse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“This inhibition became apparent after 6 to 8 hours of ethylene treatment”
e2“Pisum sativum L. cv Alaska”
Claim · model-written summary
Transport velocity was estimated at 5 millimeters per hour in both control and ethylene-treated pea tissues.
- Taxon
- Pisum sativumLathyrus oleraceuse2
- Outcome
- Transport velocitye5
- Value
- 5 millimeters per houre5
- Qualifier
- in both control and ethylene-treated tissuese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Transport velocity in both control and ethylene-treated tissues was estimated to be 5 millimeters per hour”
e2“Pisum sativum L. cv Alaska”
Claim · model-written summary
Ethylene stimulated net IAA uptake in pea tissues.
- Taxon
- Pisum sativumLathyrus oleraceuse2
- Treatment
- ethylene-treated tissuese6
- Outcome
- Net IAA uptakee6
- Direction
- stimulatede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Net IAA uptake was stimulated in ethylene-treated tissues”
e2“Pisum sativum L. cv Alaska”
Claim · model-written summary
The ability of NPA to enhance net IAA uptake was reduced in ethylene-treated pea tissues.
- Taxon
- Pisum sativumLathyrus oleraceuse2
- Treatment
- ethylenee6
- Compound
- NPAe7
- Outcome
- the relative ability of the phytotropin NPA to enhance net IAA uptakee7
- Direction
- reducede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“the relative ability of the phytotropin NPA to enhance net IAA uptake was reduced in treated tissues”
e6“Net IAA uptake was stimulated in ethylene-treated tissues”
e2“Pisum sativum L. cv Alaska”
Claim · model-written summary
Specific NPA binding to pea microsomes was saturable and consistent with a single class of binding sites.
- Taxon
- Pisum sativumLathyrus oleraceuse2
- Compound
- [(3)H]NPAe3
- Outcome
- single class of binding sitese3
- Qualifier
- saturablee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Specific binding of [(3)H]NPA to microsomes prepared from both control and ethylene-treated tissues was saturable and consistent with the existence of a single class of binding sites”
e2“Pisum sativum L. cv Alaska”
Claim · model-written summary
The apparent affinity of NPA binding in pea microsomes was 8 to 9 nanomolar.
- Taxon
- Pisum sativumLathyrus oleraceuse2
- Compound
- NPAe4
- Outcome
- apparent affinity (K(d)) toward NPAe4
- Value
- 8 to 9 nanomolare4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“with an apparent affinity (K(d)) toward NPA of 8 to 9 nanomolar”
e2“Pisum sativum L. cv Alaska”
Claim · model-written summary
Ethylene-treated pea tissues had a lower density of NPA binding sites, at 36% of control.
- Taxon
- Pisum sativumLathyrus oleraceuse2
- Treatment
- ethylene-treated tissuese5
- Outcome
- density of these binding sites (per milligram protein)e5
- Direction
- lowere5
- Value
- 36% of controle5
- Comparator
- controle5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“The density of these binding sites (per milligram protein) was lower (36% of control) in ethylene-treated tissues”
e2“Pisum sativum L. cv Alaska”
Claim · model-written summary
Direct application of ethylene to microsomes from untreated pea seedlings had no effect on specific NPA binding.
- Taxon
- Pisum sativumLathyrus oleraceuse2
- Treatment
- Direct application of ethylenee6
- Outcome
- level of specific [(3)H]NPA bindinge6
- Direction
- had no effecte6
- Comparator
- untreated seedlingse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Direct application of ethylene to microsomal preparations isolated from untreated seedlings had no effect on the level of specific [(3)H]NPA binding”
e2“Pisum sativum L. cv Alaska”
The same questions in other papers
ethene · molecular: metabolites · Lathyrus oleraceus (pea)Positive and negative results
6 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.