Paper · 1993 · Physiologia Plantarum

Developmental effects of 24‐epibrassinolide in excised roots of tomato grown in vitro

James G. Roddick; Anna L. Rijnenberg; Nobuo Ikekawa

7
claims
7
verbatim quotes
32
citations
2
research areas
—
Not open access

Abstract

The effects of 1 p M –10 μ M 24‐epibrassinolide presented to apical and basal regions of excised roots of tomato ( Lycopersicon esculentum Mill. cv. Best of All) grown aseptically in a two‐well culture vessel have been investigated. Only inhibitory effects were observed and only at 0. 1 μ M or greater. At 10 μ M basally‐ and apically‐applied epibrassinolide inhibited growth in apical regions, but not in basal regions. Lower concentrations (1 and 0. 1 μ M ) also inhibited growth, again only in apical regions and usually only when presented directly to those regions. Cultured tomato roots therefore appear to transport epibrassinolide acropetally, but whether they do so basipetally is not yet clear. The reduced responsiveness to epibrassinolide observed in roots grown by this method is thought to be due to the larger inoculum used rather than the physiological age of the roots. There was some evidence that the sensitivity of cultured roots to epibrassinolide is directly related to growth rate

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Claims 7

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Epibrassinolide inhibited root growth only at 0. 1 μ M or above.

Treatment
0. 1 μ M or greatere0
Outcome
inhibitory effectse0
Direction
inhibitorye0

Not reportedTaxonComparator

Verbatim

e0

“Only inhibitory effects were observed and only at 0. 1 μ M or greater.”

✓ located verbatim in the source · abstract, characters 233–303 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

At 10 μ M, basal and apical application inhibited growth in apical regions, but not basal regions.

Treatment
10 μ M basally‐ and apically‐applied epibrassinolidee0
Outcome
growth in apical regionse0
Direction
inhibitede0
Comparator
basal regionse0

Not reportedTaxon

Verbatim

e0

“At 10 μ M basally‐ and apically‐applied epibrassinolide inhibited growth in apical regions, but not in basal regions.”

✓ located verbatim in the source · abstract, characters 304–421 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

At 1 μ M, epibrassinolide inhibited apical region growth, usually with direct presentation.

Treatment
1e0
Outcome
growthe0
Direction
inhibitede0
Qualifier
only in apical regions and usually only when presented directly to those regionse0

Not reportedTaxonComparator

Verbatim

e0

“Lower concentrations (1 and 0. 1 μ M ) also inhibited growth, again only in apical regions and usually only when presented directly to those regions.”

✓ located verbatim in the source · abstract, characters 422–571 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

At 0. 1 μ M, epibrassinolide inhibited apical region growth, usually with direct presentation.

Treatment
0. 1 μ Me0
Outcome
growthe0
Direction
inhibitede0

Not reportedTaxonComparator

Verbatim

e0

“Lower concentrations (1 and 0. 1 μ M ) also inhibited growth, again only in apical regions and usually only when presented directly to those regions.”

✓ located verbatim in the source · abstract, characters 422–571 · show in text ↑
Observationauthor interpretationoriginal resultmeasured

Claim · model-written summary

Cultured tomato roots appear to transport epibrassinolide acropetally.

Outcome
transport epibrassinolide acropetallye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Cultured tomato roots therefore appear to transport epibrassinolide acropetally”

✓ located verbatim in the source · abstract, characters 572–651 · show in text ↑
Mechanismauthor interpretationoriginal resultnegativemeasured

Claim · model-written summary

Authors attribute reduced epibrassinolide responsiveness to larger inoculum.

Outcome
reduced responsiveness to epibrassinolidee0
Qualifier
larger inoculume0

Not reportedTaxon

Verbatim

e0

“reduced responsiveness to epibrassinolide observed in roots grown by this method is thought to be due to the larger inoculum used rather than the physiological age of the roots”

✓ located verbatim in the source · abstract, characters 710–886 · show in text ↑
Associationauthor interpretationoriginal resultmeasured

Claim · model-written summary

Cultured root sensitivity to epibrassinolide may be directly related to growth rate.

Organ / stage
cultured rootse0
Outcome
sensitivity of cultured roots to epibrassinolidee0

Not reportedTaxonDirection

Verbatim

e0

“sensitivity of cultured roots to epibrassinolide is directly related to growth rate”

✓ located verbatim in the source · abstract, characters 921–1004 · show in text ↑