Abstract
The effect of salt on ascorbate peroxidase (EC 1.11.1.11) activity and mRNA level in radish ( Raphanus sativus L. cv. Fakir) was investigated. cDNA encoding a radish ascorbate peroxidase was obtained and sequenced. Sequence data analysis showed that the coding region was well conserved among higher plants. The radish cDNA was used as a probe to measure the steady‐state level of ascorbate peroxidase mRNA in plants subjected to salt stress (100 m M NaCl). The levels of transcripts encoding ascorbate peroxidase were almost identical in control or salt‐grown radish plants in both leaves and roots. The activity of soluble ascorbate peroxidase was enhanced by the salt treatment in both leaves and roots. The salt‐induced increase in ascorbate peroxidase activity requires days to become significant and can be considered as a late response. The increase in activity, but not in mRNA level, suggests that the salt‐induced ascorbate peroxidase expression is probably the consequence of post‐transcriptional events.
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Claims 6
Claim · model-written summary
A radish ascorbate peroxidase cDNA was obtained and sequenced.
- Taxon
- radish
Raphanus sativuse0 - Gene
- ascorbate peroxidasee0
- Outcome
- cDNA encoding a radish ascorbate peroxidasee0
- Value
- cDNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cDNA encoding a radish ascorbate peroxidase was obtained and sequenced.”
Claim · model-written summary
The ascorbate peroxidase coding region was conserved among higher plants.
- Taxon
- radishRaphanus sativuse1
- Gene
- ascorbate peroxidasee1
- Outcome
- coding regione0
- Direction
- well conservede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the coding region was well conserved among higher plants.”
e1“radish ascorbate peroxidase was obtained and sequenced.”
Claim · model-written summary
Salt-grown and control radish had almost identical ascorbate peroxidase transcript levels in leaves and roots.
- Taxon
- radishRaphanus sativuse0
- Organ / stage
- leaves and rootse0
- Treatment
- salt‐growne0
- Outcome
- levels of transcripts encoding ascorbate peroxidasee0
- Direction
- almost identicale0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The levels of transcripts encoding ascorbate peroxidase were almost identical in control or salt‐grown radish plants in both leaves and roots.”
Claim · model-written summary
Salt treatment enhanced soluble ascorbate peroxidase activity in radish leaves and roots.
- Taxon
- radishRaphanus sativuse1
- Organ / stage
- leaves and rootse0
- Treatment
- salt treatmente0
- Outcome
- activity of soluble ascorbate peroxidasee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activity of soluble ascorbate peroxidase was enhanced by the salt treatment in both leaves and roots.”
e1“radish ( Raphanus sativus L. cv. Fakir)”
Claim · model-written summary
Salt-induced enzyme activity took days to become significant.
- Taxon
- radishRaphanus sativuse1
- Outcome
- ascorbate peroxidase activitye0
- Direction
- increasee0
- Qualifier
- requires dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The salt‐induced increase in ascorbate peroxidase activity requires days to become significant”
e1“radish ( Raphanus sativus L. cv. Fakir)”
Claim · model-written summary
The authors attribute higher enzyme activity without mRNA change to post-transcriptional events.
- Taxon
- radishRaphanus sativuse1
- Outcome
- salt‐induced ascorbate peroxidase expressione0
- Qualifier
- post‐transcriptional eventse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The increase in activity, but not in mRNA level, suggests that the salt‐induced ascorbate peroxidase expression is probably the consequence of post‐transcriptional events.”
e1“radish ( Raphanus sativus L. cv. Fakir)”
The same questions in other papers
salinity · physiology: antioxidant enzymes · Raphanus sativus (radish)Results differ
2 in the opposite direction
- negative
- no effect
salinity · molecular: expression · Raphanus sativus (radish)Positive and negative results
2 in the opposite direction
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.