Paper · 2013 · TURKISH JOURNAL OF AGRICULTURE AND FORESTRY

Oxidative stress and antioxidant defense mechanism in mung bean seedlings after lead and cadmium treatments

Meher HASSAN; Simeen MANSOOR

9
claims
20
verbatim quotes
57
citations
1
research areas
✓
Open access

Abstract

This research was an attempt to study the effect of heavy metals lead and cadmium (0.05 mM and 0.3 mM) on growth and antioxidant enzymes of seedlings of 2 mung bean genotypes (NM 19-19 and Azri mung-2006). Results revealed that germination percentage and seedling length decreased when compared with the control for both genotypes. However, seedling length and germination percentage was better in NM 19-19 as compared to Azri mung-2006. Elevated levels of protein were observed under metal stress in both genotypes. Heavy metals induced oxidative stress in plants, causing membrane injury as observed by enhanced level of malondialdehyde (MDA) contents. Increase in antioxidant enzymes activity was detected for guaiacol peroxidase (GPX) and catalase (CAT). However, ascorbate (APX) activity decreased under stress for both genotypes. We observed more MDA content and GPX and APX activity in Azri mung-2006 as compared to NM 19-19 when high concentrations of Pb and Cd were added. This revealed that NM 19-19 was tolerant whereas Azri mung-2006 was sensitive to Pb and Cd. It was further noticed that Cd imposed a more deleterious effect than Pb on both genotypes.

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Claims 9

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Metal treatment reduced germination percentage and seedling length in both genotypes.

Taxon
mung beanVigna radiatat0
Treatment
lead and cadmiume1
Outcome
germination percentage and seedling lengthe0
Direction
decreasede0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Results revealed that germination percentage and seedling length decreased when compared with the control for both genotypes.”

✓ located verbatim in the source · abstract, characters 206–331 · show in text ↑
e1

“heavy metals lead and cadmium (0.05 mM and 0.3 mM)”

✓ located verbatim in the source · abstract, characters 52–102 · show in text ↑
t0

“mung bean”

✓ located verbatim in the source · title, characters 54–63 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

NM 19-19 had better seedling length and germination than Azri mung-2006.

Taxon
mung beanVigna radiatat0
Material
NM 19-19e0
Outcome
seedling length and germination percentagee0
Direction
bettere0
Comparator
Azri mung-2006e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“seedling length and germination percentage was better in NM 19-19 as compared to Azri mung-2006.”

✓ located verbatim in the source · abstract, characters 341–437 · show in text ↑
t0

“mung bean”

✓ located verbatim in the source · title, characters 54–63 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Metal stress elevated protein levels in both genotypes.

Taxon
mung beanVigna radiatat0
Treatment
metal stresse0
Outcome
levels of proteine0
Direction
Elevatede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Elevated levels of protein were observed under metal stress in both genotypes.”

✓ located verbatim in the source · abstract, characters 438–516 · show in text ↑
t0

“mung bean”

✓ located verbatim in the source · title, characters 54–63 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Heavy metals increased malondialdehyde content, indicating membrane injury.

Taxon
mung beanVigna radiatat0
Treatment
Heavy metalse0
Outcome
malondialdehyde (MDA) contentse0
Direction
enhancede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Heavy metals induced oxidative stress in plants, causing membrane injury as observed by enhanced level of malondialdehyde (MDA) contents.”

✓ located verbatim in the source · abstract, characters 517–654 · show in text ↑
t0

“mung bean”

✓ located verbatim in the source · title, characters 54–63 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Guaiacol peroxidase and catalase activities increased under metal stress.

Taxon
mung beanVigna radiatat0
Treatment
metal stresse1
Gene
guaiacol peroxidase (GPX) and catalase (CAT)e0
Outcome
antioxidant enzymes activitye0
Direction
Increasee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Increase in antioxidant enzymes activity was detected for guaiacol peroxidase (GPX) and catalase (CAT).”

✓ located verbatim in the source · abstract, characters 655–758 · show in text ↑
e1

“under metal stress in both genotypes.”

✓ located verbatim in the source · abstract, characters 479–516 · show in text ↑
t0

“mung bean”

✓ located verbatim in the source · title, characters 54–63 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Ascorbate APX activity decreased under stress in both genotypes.

Taxon
mung beanVigna radiatat0
Treatment
stresse0
Outcome
ascorbate (APX) activitye0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“ascorbate (APX) activity decreased under stress for both genotypes.”

✓ located verbatim in the source · abstract, characters 768–835 · show in text ↑
t0

“mung bean”

✓ located verbatim in the source · title, characters 54–63 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Azri mung-2006 had more MDA content and GPX and APX activity than NM 19-19 at high Pb and Cd concentrations.

Taxon
mung beanVigna radiatat0
Material
Azri mung-2006e0
Treatment
Pb and Cde0
Outcome
MDA content and GPX and APX activitye0
Direction
moree0
Comparator
NM 19-19e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“more MDA content and GPX and APX activity in Azri mung-2006 as compared to NM 19-19 when high concentrations of Pb and Cd were added.”

✓ located verbatim in the source · abstract, characters 848–981 · show in text ↑
t0

“mung bean”

✓ located verbatim in the source · title, characters 54–63 · show in text ↑
Observationauthor interpretationoriginal resultmeasured

Claim · model-written summary

NM 19-19 was tolerant whereas Azri mung-2006 was sensitive to Pb and Cd.

Taxon
mung beanVigna radiatat0
Material
NM 19-19e0
Treatment
Pb and Cde0
Outcome
tolerante0
Comparator
Azri mung-2006e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“NM 19-19 was tolerant whereas Azri mung-2006 was sensitive to Pb and Cd.”

✓ located verbatim in the source · abstract, characters 1001–1073 · show in text ↑
t0

“mung bean”

✓ located verbatim in the source · title, characters 54–63 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Cadmium was more deleterious than lead for both genotypes.

Taxon
mung beanVigna radiatat0
Treatment
Cde0
Outcome
deleterious effecte0
Direction
moree0
Comparator
Pbe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Cd imposed a more deleterious effect than Pb on both genotypes.”

✓ located verbatim in the source · abstract, characters 1102–1165 · show in text ↑
t0

“mung bean”

✓ located verbatim in the source · title, characters 54–63 · show in text ↑

lead atom · growth · Vigna radiata (mung bean)Results differ

10 papers: 1 positive, 9 negative or no effect

8 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. negative

6 papers more in this group

See all 10 papers →

cadmium atom · growth · Vigna radiata (mung bean)Results agree

21 papers: 0 positive, 21 negative or no effect

20 papers in the same direction as this paper

  1. negative
  2. negative
  3. negative

17 papers more in this group

See all 21 papers →

heavy metal · physiology: oxidative · Vigna radiata (mung bean)Results agree

6 papers: 0 positive, 6 negative or no effect

5 papers in the same direction as this paper

  1. negative
  2. negative
  3. negative

2 papers more in this group

See all 6 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.