Abstract
Early events of multiple bud formation and shoot development in germinating soybean embryonic axes treated for 24 hr with the cytokinin, 6‐benzylaminopurine (BAP), were compared to the development of untreated control axes using four different techniques: photomicrography, scanning electron microscopy, histology, and autoradiography. Shoot apex development in BAP‐treated embryonic axes was delayed by about 9 to 15 hr. A transient inhibition of DNA synthesis in the primary apical meristem and axillary buds was observed with subsequent changes in the timing of cell division patterns in these regions. Meristematic regions (supernumerary vegetative buds) were observed in BAP‐treated axes around the perimeter of the apical dome at and above the level of the axillary buds. Cells elongated from some of the BAP‐induced meristematic regions to form four to six shoots. In the absence of BAP, excision of the primary apical meristem and/or axillary buds did not result in multiple bud formation. These results suggest that transient exposure to BAP interrupted chromosomal DNA replication and reprogrammed the developmental fate of a large number of cells in the shoot apex. We postulate that interruption of DNA synthesis, either directly, by interfering with DNA replication, or indirectly, by preventing entry into S‐phase, effected redetermination of the shoot apex cells.
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Claims 7
Claim · model-written summary
BAP delayed shoot apex development by about 9 to 15 hr.
- Taxon
- soybean
Glycine maxt0 - Treatment
- BAP‐treatede0
- Outcome
- Shoot apex developmente0
- Direction
- delayede0
- Value
- about 9 to 15 hre0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot apex development in BAP‐treated embryonic axes was delayed by about 9 to 15 hr”
t0“Early events of multiple bud formation and shoot development in soybean embryonic axes treated with the cytokinin, 6‐benzylaminopurine”
Claim · model-written summary
BAP transiently inhibited DNA synthesis in primary apical meristem and axillary buds.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- primary apical meristem and axillary budse0
- Treatment
- 6‐benzylaminopurinet0
- Outcome
- DNA synthesise0
- Direction
- inhibitione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A transient inhibition of DNA synthesis in the primary apical meristem and axillary buds was observed”
t0“Early events of multiple bud formation and shoot development in soybean embryonic axes treated with the cytokinin, 6‐benzylaminopurine”
Claim · model-written summary
Cell division timing changed in these meristematic regions.
- Taxon
- soybeanGlycine maxt0
- Outcome
- timing of cell division patternse0
- Direction
- changese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“subsequent changes in the timing of cell division patterns in these regions”
t0“Early events of multiple bud formation and shoot development in soybean embryonic axes treated with the cytokinin, 6‐benzylaminopurine”
Claim · model-written summary
BAP treated axes formed supernumerary vegetative buds around the apical dome.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- apical domee0
- Treatment
- BAP‐treatede0
- Outcome
- supernumerary vegetative budse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Meristematic regions (supernumerary vegetative buds) were observed in BAP‐treated axes around the perimeter of the apical dome at and above the level of the axillary buds”
t0“Early events of multiple bud formation and shoot development in soybean embryonic axes treated with the cytokinin, 6‐benzylaminopurine”
Claim · model-written summary
Some BAP induced meristematic regions formed four to six shoots.
- Taxon
- soybeanGlycine maxt0
- Treatment
- BAP‐inducede0
- Outcome
- four to six shootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cells elongated from some of the BAP‐induced meristematic regions to form four to six shoots”
t0“Early events of multiple bud formation and shoot development in soybean embryonic axes treated with the cytokinin, 6‐benzylaminopurine”
Claim · model-written summary
Meristem or axillary bud excision without BAP did not induce multiple buds.
- Taxon
- soybeanGlycine maxt0
- Treatment
- excision of the primary apical meristem and/or axillary budse0
- Outcome
- multiple bud formatione0
- Comparator
- absence of BAPe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the absence of BAP, excision of the primary apical meristem and/or axillary buds did not result in multiple bud formation”
t0“Early events of multiple bud formation and shoot development in soybean embryonic axes treated with the cytokinin, 6‐benzylaminopurine”
Claim · model-written summary
Authors infer BAP interrupted DNA replication and reprogrammed shoot apex cell fate.
- Taxon
- soybeanGlycine maxt0
- Treatment
- transient exposure to BAPe0
- Outcome
- developmental fate of a large number of cells in the shoot apexe0
- Qualifier
- interrupted chromosomal DNA replicatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient exposure to BAP interrupted chromosomal DNA replication and reprogrammed the developmental fate of a large number of cells in the shoot apex”
t0“Early events of multiple bud formation and shoot development in soybean embryonic axes treated with the cytokinin, 6‐benzylaminopurine”
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