Abstract
Male fertility of crop plants is a function of pollen production and viability. Differences in pollen production can be evaluated by simple observation, but pollen viability is more difficult to quantify. The objective of this study was to develop an in vitro pollen germination assay for sorghum [ Sorghum bicolor (L.) Moench]. In experiments evaluating common germination media substrates, large differences in germination were observed in response to changing concentrations of sucrose, boric acid, and calcium nitrate in agar‐based media. In vitro germination was maximized following incubation on agar supplemented with 0.9 M sucrose, 2.43 m M boric acid, and 2.12 m M calcium nitrate. The effect of incubation temperature was also significant. Pollen germination was not affected by changes in temperature between 20 and 40°C, but germination was significantly reduced at 10°C.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Sorghum pollen germination differed substantially across sucrose, boric acid, and calcium nitrate concentrations.
- Treatment
- changing concentrations of sucrose, boric acid, and calcium nitratee0
- Outcome
- germinatione0
- Direction
- large differencese0
SorghumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“large differences in germination were observed in response to changing concentrations of sucrose, boric acid, and calcium nitrate in agar‐based media”
t0“Estimation of Pollen Viability in Grain Sorghum”
Claim · model-written summary
Germination was highest on agar containing 0.9 M sucrose, 2.43 m M boric acid, and 2.12 m M calcium nitrate.
- Treatment
- agar supplemented with 0.9 M sucrose, 2.43 m M boric acid, and 2.12 m M calcium nitratee0
- Outcome
- In vitro germinatione0
- Direction
- maximizede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro germination was maximized following incubation on agar supplemented with 0.9 M sucrose, 2.43 m M boric acid, and 2.12 m M calcium nitrate”
t0“Estimation of Pollen Viability in Grain Sorghum”
Claim · model-written summary
Incubation temperature significantly affected sorghum pollen germination.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effect of incubation temperature was also significant”
t0“Estimation of Pollen Viability in Grain Sorghum”
Claim · model-written summary
Pollen germination did not change across 20 to 40°C.
- Treatment
- changes in temperature between 20 and 40°Ce0
- Outcome
- Pollen germinatione0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pollen germination was not affected by changes in temperature between 20 and 40°C”
t0“Estimation of Pollen Viability in Grain Sorghum”
Claim · model-written summary
Pollen germination was significantly reduced at 10°C.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“germination was significantly reduced at 10°C”
t0“Estimation of Pollen Viability in Grain Sorghum”
The same questions in other papers
sucrose · growth · SorghumPositive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
2 papers more in this group
See all 6 papers →calcium nitrate · growth · SorghumResults agree
1 paper in the same direction as this paper
- positive
agar · growth · SorghumResults agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.