Abstract
Summary Foliage of potato cv. Desiree was harvested from glasshouse‐cultivated plants of five experimental transgenic lines expressing three different insecticidal proteins (snowdrop lectin, Galanthus nivalis agglutinin (GNA); jackbean lectin, Concanavalin A (Con A), cowpea trypsin inhibitor; (CpTi)), tissue‐cultured control plants and standard control (non‐tissue cultured) plants. The foliage was subdivided into stems, upper, middle and lower leaves and analysed separately by HPLC for the solanidine‐based glycoalkaloids a‐solanine and a‐chaconine. The results demonstrate that one or more stages in the plant transformation process (i.e. insecticidal‐ and marker‐gene insertions, gene expression and tissue culture) resulted in a lower level of leaf glycoalkaloids than that found in either the tissue‐cultured controls or standard controls, based on the selected potato lines transformed for insecticidal protein expression. However, the distribution of glycoalkaloids throughout the plant foliage was unaffected by genetic transformation and tissue culture, with the highest glycoalkaloid levels being observed in the top third of the plant. The importance of investigating unexpected effects of genetic engineering on plant secondary metabolism is discussed from an ecological viewpoint.
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Claims 3
Claim · model-written summary
One or more stages of the plant transformation process lowered leaf glycoalkaloid levels relative to tissue-cultured and standard controls.
- Taxon
- potato
Solanum tuberosume3 - Material
- transgenic linese3
- Organ / stage
- leafe1
- Treatment
- plant transformation processe0
- Outcome
- leaf glycoalkaloidse1
- Direction
- lowere1
- Comparator
- standard controlse2
- Setting
- glasshouse‐cultivated plantse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results demonstrate that one or more stages in the plant transformation process”
e1“resulted in a lower level of leaf glycoalkaloids”
e2“or standard controls”
e3“Foliage of potato cv. Desiree was harvested from glasshouse‐cultivated plants of five experimental transgenic lines”
Claim · model-written summary
Genetic transformation and tissue culture did not change the distribution of glycoalkaloids throughout the potato foliage.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- genetic transformation and tissue culturee0
- Outcome
- distribution of glycoalkaloids throughout the plant foliagee0
- Direction
- unaffectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the distribution of glycoalkaloids throughout the plant foliage was unaffected by genetic transformation and tissue culture”
e1“Foliage of potato cv. Desiree was harvested from glasshouse‐cultivated plants of five experimental transgenic lines”
Claim · model-written summary
The highest glycoalkaloid levels in potato foliage were observed in the top third of the plant.
- Taxon
- potatoSolanum tuberosume1
- Organ / stage
- top third of the plante0
- Outcome
- glycoalkaloid levelse0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with the highest glycoalkaloid levels being observed in the top third of the plant”
e1“Foliage of potato cv. Desiree was harvested from glasshouse‐cultivated plants of five experimental transgenic lines”
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