Abstract
Explants of stem, leaves, roots, and cotyledons from etiolated axenically grown Vicia faba seedlings were cultured on a range of media. Shoot organogenesis was only obtained with nodal stem and cotyledonary node explants when cultured on MS medium with 3% sucrose, 2.0 mg 1−1 BAP and 02 mg 1−1 NAA. Callus proliferation accompanied shoot organogenesis from nodal stem explants. Successive subculture of nodal stem callus resulted in proliferation of regenerative callus which contained several shoot bud initials. The capacity for shoot regeneration from this callus was maintained for 9 months. Histological studies reveal de novo formation of meristematic centres in callus and their further development into bud primordia. High frequency rooting of these adventitious shoots was obtained on half-strength MS medium with 1.5% sucrose, 0.1 mg 1−1 NAA and 0.5 mg 1−1 kinetin.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Shoot organogenesis occurred only in nodal stem and cotyledonary node explants on the specified MS medium.
- Taxon
- Vicia faba
Vicia fabat0 - Organ / stage
- nodal stem and cotyledonary node explantse0
- Treatment
- MS medium with 3% sucrose, 2.0 mg 1−1 BAP and 02 mg 1−1 NAAe0
- Outcome
- Shoot organogenesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot organogenesis was only obtained with nodal stem and cotyledonary node explants when cultured on MS medium with 3% sucrose, 2.0 mg 1−1 BAP and 02 mg 1−1 NAA”
t0“Vicia faba”
Claim · model-written summary
Callus proliferation accompanied shoot organogenesis from nodal stem explants.
- Taxon
- Vicia fabaVicia fabat0
- Organ / stage
- nodal stem explantse0
- Outcome
- Callus proliferatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus proliferation accompanied shoot organogenesis from nodal stem explants”
t0“Vicia faba”
Claim · model-written summary
Successive nodal stem callus subculture yielded regenerative callus with shoot bud initials.
- Taxon
- Vicia fabaVicia fabat0
- Organ / stage
- nodal stem calluse0
- Treatment
- Successive subculturee0
- Outcome
- proliferation of regenerative calluse0
- Direction
- proliferatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Successive subculture of nodal stem callus resulted in proliferation of regenerative callus which contained several shoot bud initials”
t0“Vicia faba”
Claim · model-written summary
Shoot regeneration capacity of the callus persisted for nine months.
- Taxon
- Vicia fabaVicia fabat0
- Outcome
- capacity for shoot regeneratione0
- Direction
- maintainede0
- Value
- 9 monthse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The capacity for shoot regeneration from this callus was maintained for 9 months”
t0“Vicia faba”
Claim · model-written summary
Meristematic centres formed de novo in callus and developed into bud primordia.
- Taxon
- Vicia fabaVicia fabat0
- Outcome
- de novo formation of meristematic centres in callus and their further development into bud primordiae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“de novo formation of meristematic centres in callus and their further development into bud primordia”
t0“Vicia faba”
Claim · model-written summary
Adventitious shoots rooted at high frequency on the specified half-strength MS medium.
- Taxon
- Vicia fabaVicia fabat0
- Organ / stage
- adventitious shootse0
- Treatment
- half-strength MS medium with 1.5% sucrose, 0.1 mg 1−1 NAA and 0.5 mg 1−1 kinetine0
- Outcome
- High frequency rootinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High frequency rooting of these adventitious shoots was obtained on half-strength MS medium with 1.5% sucrose, 0.1 mg 1−1 NAA and 0.5 mg 1−1 kinetin”
t0“Vicia faba”
The same questions in other papers
in vitro · growth · Vicia faba (faba bean)Positive and negative results
3 in the opposite direction
- negative
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.