Abstract
The use of promoter-reporter gene constructs in transgenic plants is a powerful tool in the analysis of gene expression which can, however, be limited in the resolution of small structures, such as the apical meristem. This paper reports on a fluorescent imaging technique for the analysis of GUS reporter gene expression to cellular resolution in the apical meristem of tomato. Using this technique in combination with an RT-PCR analysis of RBS gene-specific transcript levels, it is shown that: 5' upstream sequences of RBCS genes are sufficient to mimic the pattern of transcripts revealed by in situ hybridisation (no expression in the apical meristem, high expression in the initiated leaf primordia); the genes RBCS2, RBCS3A and RBCS3B are transcriptionally activated upon primordium initiation with transcripts for RBCS1 and RBCS3C accumulating later in leaf development; and that RBCS promoter activity cannot be induced in the apical meristem by light, an environmental signal which elevates RBCS transcript level in other aerial parts of the plant. These data provide a detailed picture of the complex transcriptional events occurring on leaf initiation and the establishment of the photosynthetic machinery; they describe two complementary techniques which allow the analysis of such complex events at the tissue and cellular level; and they characterize an in vivo assay system which can be used to analyse the factors involved in the initiation and maintenance of gene expression patterns in the apical meristem.
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Claims 5
Claim · model-written summary
A fluorescent imaging technique allows analysis of GUS reporter gene expression to cellular resolution in the apical meristem of tomato.
- Taxon
- tomato
Solanum lycopersicume1 - Organ / stage
- apical meristeme1
- Outcome
- GUS reporter gene expressione1
- Value
- cellular resolutione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a fluorescent imaging technique for the analysis of GUS reporter gene expression to cellular resolution in the apical meristem of tomato”
Claim · model-written summary
5' upstream sequences of RBCS genes are sufficient to mimic the pattern of transcripts seen by in situ hybridisation (no expression in the apical meristem, high expression in initiated leaf primordia) in tomato.
- Taxon
- tomatoSolanum lycopersicume2
- Gene
- RBCS genese1
- Outcome
- pattern of transcriptse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“5' upstream sequences of RBCS genes are sufficient to mimic the pattern of transcripts revealed by in situ hybridisation (no expression in the apical meristem, high expression in the initiated leaf primordia)”
e2“apical meristem of tomato”
Claim · model-written summary
In tomato, RBCS2, RBCS3A and RBCS3B are transcriptionally activated upon leaf primordium initiation.
- Taxon
- tomatoSolanum lycopersicume2
- Gene
- RBCS2, RBCS3A and RBCS3Be1
- Outcome
- transcriptionally activated upon primordium initiatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the genes RBCS2, RBCS3A and RBCS3B are transcriptionally activated upon primordium initiation”
e2“apical meristem of tomato”
Claim · model-written summary
In tomato, transcripts for RBCS1 and RBCS3C accumulate later in leaf development.
- Taxon
- tomatoSolanum lycopersicume2
- Gene
- RBCS1 and RBCS3Ce1
- Outcome
- transcriptse1
- Qualifier
- later in leaf developmente1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“transcripts for RBCS1 and RBCS3C accumulating later in leaf development”
e2“apical meristem of tomato”
Claim · model-written summary
Light does not induce RBCS promoter activity in the tomato apical meristem, although light elevates RBCS transcript level in other aerial parts.
- Taxon
- tomatoSolanum lycopersicume2
- Organ / stage
- apical meristeme1
- Treatment
- lighte1
- Outcome
- RBCS promoter activitye1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“RBCS promoter activity cannot be induced in the apical meristem by light”
e2“apical meristem of tomato”
The same questions in other papers
light · molecular: expression · Solanum lycopersicum (tomato)Positive and negative results
7 papers in the same direction as this paper · 17 in the opposite direction
- positive
- positive
- positive
21 papers more in this group
See all 25 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.