Paper · 2014 · Plant Breeding

Conversion of the genic male sterility (GMS) system of bell pepper (Capsicum annuumL.) to cytoplasmic male sterility (CMS)

Mulyantoro; Shang‐Ling Ou; Shu‐Yun Chen; et al.

3
claims
5
verbatim quotes
8
citations
3
research areas
—
Not open access

Abstract

Abstract Non‐pungent bell pepper ( Capsicum annuum L.) lacks the cytoplasmic male sterility ( CMS ) nuclear restorer allele, Rf , and CMS cannot be employed in its F 1 hybrid seed production. To demonstrate that the genic male sterility ( GMS ) system in non‐pungent bell pepper can be converted to the CMS male sterility system, the conversion of GMS to CMS for non‐pungent bell pepper line GC 3 was conducted by introgression of S‐type cytoplasm and the Rf allele from tropical pungent donors. After morphological traits were evaluated, two lines from BC 1 F 1 containing S‐type cytoplasm and four lines from BC 2 F 2 containing Rf allele, phenotypically similar to GC 3, were obtained and could be employed as CMS male sterile lines and restorer lines for non‐pungent bell pepper. Four molecular markers potentially linked to traits of interest were also evaluated in BC 1 F 1 and BC 1 F 2 populations. This is the first time that GMS has been successfully converted to CMS in bell pepper, a significant contribution for bell pepper hybrid seed production.

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Claims 3

Observationobservedoriginal resultmeasured

Claim · model-written summary

Two S-type cytoplasm lines resembling GC 3 were obtained.

Taxon
bell peppert0
Material
BC 1 F 1e0
Gene
S‐type cytoplasme0
Outcome
two lines from BC 1 F 1 containing S‐type cytoplasme0

Verbatim

e0

“two lines from BC 1 F 1 containing S‐type cytoplasm and four lines from BC 2 F 2 containing Rf allele, phenotypically similar to GC 3, were obtained”

✓ located verbatim in the source · abstract, characters 539–687 · show in text ↑
t0

“bell pepper”

✓ located verbatim in the source · title, characters 55–66 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Four Rf-bearing lines resembling GC 3 were obtained.

Taxon
bell peppert0
Material
BC 2 F 2e0
Gene
Rf allelee0
Outcome
four lines from BC 2 F 2 containing Rf allelee0

Verbatim

e0

“four lines from BC 2 F 2 containing Rf allele, phenotypically similar to GC 3, were obtained”

✓ located verbatim in the source · abstract, characters 595–687 · show in text ↑
t0

“bell pepper”

✓ located verbatim in the source · title, characters 55–66 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

GMS was successfully converted to CMS in bell pepper.

Taxon
bell peppere0
Outcome
GMS has been successfully converted to CMSe0

Verbatim

e0

“GMS has been successfully converted to CMS in bell pepper”

✓ located verbatim in the source · abstract, characters 934–991 · show in text ↑