Paper · 2007 · Biologia Plantarum

Effect of NaCl and CaCl2 on growth and contents of minerals, chlorophyll, proline and sugars in the apple rootstock M 4 cultured in vitro

T. E. Sotiropoulos

6
claims
12
verbatim quotes
58
citations
2
research areas
✓
Open access

Abstract

The apple (Malus domestica Borkh) rootstock M 4 shoots were grown in vitro for 4 weeks on Murashige and Skoog (MS) medium containing three NaCl concentrations (35, 100 and 200 mM) in combination with two CaCl2 concentrations (5 and 10 mM). Inclusion of 10 mM CaCl2 in the medium, in the presence of 35 mM NaCl, significantly increased the number of shoots and the fresh mass compared to 5 mM CaCl2. The number of shoots, length of shoots, and the fresh mass of cultures were very low in the presence of 100 and 200 mM NaCl, independently of CaCl2 concentration of the medium. By increasing NaCl and CaCl2 concentrations in the culture medium, contents of N, Na, Cl, proline and soluble sugars in plantlets increased, whereas K, Mg, B, Zn and chlorophyll content decreased in comparison to the control.

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Claims 6

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

At 35 mM NaCl, 10 mM CaCl2 significantly increased shoot number and fresh mass compared with 5 mM CaCl2.

Material
M 4t0
Treatment
10 mM CaCl2e0
Outcome
number of shoots and the fresh masse0
Direction
increasede0
Qualifier
35 mM NaCle0
Comparator
5 mM CaCl2e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Inclusion of 10 mM CaCl2 in the medium, in the presence of 35 mM NaCl, significantly increased the number of shoots and the fresh mass compared to 5 mM CaCl2.”

✓ located verbatim in the source · abstract, characters 240–398 · show in text ↑
t0

“apple rootstock M 4”

✓ located verbatim in the source · title, characters 100–119 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Shoot number, shoot length and culture fresh mass were very low at 100 and 200 mM NaCl regardless of CaCl2 concentration.

Material
M 4t0
Treatment
100 and 200 mM NaCle0
Outcome
number of shoots, length of shoots, and the fresh mass of culturese0
Direction
very lowe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“The number of shoots, length of shoots, and the fresh mass of cultures were very low in the presence of 100 and 200 mM NaCl, independently of CaCl2 concentration of the medium.”

✓ located verbatim in the source · abstract, characters 399–575 · show in text ↑
t0

“apple rootstock M 4”

✓ located verbatim in the source · title, characters 100–119 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Increasing medium NaCl and CaCl2 increased plantlet N, Na and Cl contents relative to control.

Material
M 4t0
Treatment
increasing NaCl and CaCl2 concentrationse0
Outcome
contents of N, Na, Cle0
Direction
increasede0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“By increasing NaCl and CaCl2 concentrations in the culture medium, contents of N, Na, Cl, proline and soluble sugars in plantlets increased, whereas K, Mg, B, Zn and chlorophyll content decreased in comparison to the control.”

✓ located verbatim in the source · abstract, characters 576–801 · show in text ↑
t0

“apple rootstock M 4”

✓ located verbatim in the source · title, characters 100–119 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Increasing medium NaCl and CaCl2 increased plantlet proline and soluble sugars relative to control.

Material
M 4t0
Treatment
increasing NaCl and CaCl2 concentrationse0
Outcome
proline and soluble sugarse0
Direction
increasede0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“By increasing NaCl and CaCl2 concentrations in the culture medium, contents of N, Na, Cl, proline and soluble sugars in plantlets increased, whereas K, Mg, B, Zn and chlorophyll content decreased in comparison to the control.”

✓ located verbatim in the source · abstract, characters 576–801 · show in text ↑
t0

“apple rootstock M 4”

✓ located verbatim in the source · title, characters 100–119 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Increasing medium NaCl and CaCl2 decreased plantlet K, Mg and B contents relative to control.

Material
M 4t0
Treatment
increasing NaCl and CaCl2 concentrationse0
Outcome
K, Mg, Be0
Direction
decreasede0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“By increasing NaCl and CaCl2 concentrations in the culture medium, contents of N, Na, Cl, proline and soluble sugars in plantlets increased, whereas K, Mg, B, Zn and chlorophyll content decreased in comparison to the control.”

✓ located verbatim in the source · abstract, characters 576–801 · show in text ↑
t0

“apple rootstock M 4”

✓ located verbatim in the source · title, characters 100–119 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Increasing medium NaCl and CaCl2 decreased plantlet Zn and chlorophyll content relative to control.

Material
M 4t0
Treatment
increasing NaCl and CaCl2 concentrationse0
Outcome
Zn and chlorophyll contente0
Direction
decreasede0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“By increasing NaCl and CaCl2 concentrations in the culture medium, contents of N, Na, Cl, proline and soluble sugars in plantlets increased, whereas K, Mg, B, Zn and chlorophyll content decreased in comparison to the control.”

✓ located verbatim in the source · abstract, characters 576–801 · show in text ↑
t0

“apple rootstock M 4”

✓ located verbatim in the source · title, characters 100–119 · show in text ↑

calcium chloride dihydrate · growth · Malus domestica (apple)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
See all 3 papers →

calcium chloride dihydrate · nutrient: content · Malus domestica (apple)Positive and negative results

32 papers: 25 positive, 7 negative or no effect

6 papers in the same direction as this paper · 25 in the opposite direction

  1. positive
  2. positive
  3. positive

28 papers more in this group

See all 32 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.