Abstract
In higher plants formate dehydrogenase (FDH, EC 1.2.1.2.) is a mitochondrial, NAD-dependent enzyme. We previously reported that in potato (Solanum tuberosum L.) FDH expression is high in tubers but low in green leaves. Here we show that in isolated tuber mitochondria FDH is involved in formate-dependent O2 uptake coupled to ATP synthesis. The effects of various environmental and chemical factors on FDH expression in leaves were tested using the mitochondrial serine hydroxymethyltransferase as a control. The abundance of FDH transcripts is strongly increased under various stresses, whereas serine hydroxymethyltransferase transcripts decline. The application of formate to leaves strongly enhances FDH expression, suggesting that it might be the signal for FDH induction. Our experiments using glycolytic products suggest that glycolysis may play an important role in formate synthesis in leaves in the dark and during hypoxia, and in tubers. Of particular interest is the dramatic accumulation of FDH transcripts after spraying methanol on leaves, as this compound is known to increase the yields of C3 plants. In addition, although the steady-state levels of FDH transcript increase very quickly in response to stress, protein accumulation is much slower, but can eventually reach the same levels in leaves as in tubers.
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Claims 9
Claim · model-written summary
Tuber mitochondrial FDH participates in formate dependent oxygen uptake coupled to ATP synthesis.
- Taxon
- potato
Solanum tuberosumt0 - Organ / stage
- tubere0
- Gene
- FDHe0
- Outcome
- formate-dependent O2 uptake coupled to ATP synthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in isolated tuber mitochondria FDH is involved in formate-dependent O2 uptake coupled to ATP synthesis.”
t0“Potato”
Claim · model-written summary
FDH transcripts increased strongly under several stresses.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- various stressese0
- Gene
- FDHe0
- Outcome
- abundance of FDH transcriptse0
- Direction
- strongly increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The abundance of FDH transcripts is strongly increased under various stresses”
t0“Potato”
Claim · model-written summary
Serine hydroxymethyltransferase transcripts declined under the tested stresses.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- serine hydroxymethyltransferasee0
- Outcome
- serine hydroxymethyltransferase transcriptse0
- Direction
- declinee0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“whereas serine hydroxymethyltransferase transcripts decline.”
t0“Potato”
Claim · model-written summary
Formate application strongly enhanced FDH expression.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- application of formate to leavese0
- Gene
- FDHe0
- Outcome
- FDH expressione0
- Direction
- strongly enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The application of formate to leaves strongly enhances FDH expression”
t0“Potato”
Claim · model-written summary
Formate may signal FDH induction.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- FDHe0
- Outcome
- FDH inductione0
- Qualifier
- signale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting that it might be the signal for FDH induction.”
t0“Potato”
Claim · model-written summary
Glycolysis may contribute to formate synthesis in dark or hypoxic leaves and tubers.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- leavese0
- Outcome
- formate synthesise0
- Qualifier
- glycolysise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glycolysis may play an important role in formate synthesis in leaves in the dark and during hypoxia, and in tubers.”
t0“Potato”
Claim · model-written summary
Methanol spraying caused dramatic FDH transcript accumulation in leaves.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- spraying methanol on leavese0
- Gene
- FDHe0
- Outcome
- FDH transcriptse0
- Direction
- dramatic accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dramatic accumulation of FDH transcripts after spraying methanol on leaves”
t0“Potato”
Claim · model-written summary
FDH transcript levels rose quickly under stress while protein accumulation was slower.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- FDHe0
- Outcome
- steady-state levels of FDH transcripte0
- Direction
- increase very quicklye0
- Qualifier
- protein accumulation is much slowere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“although the steady-state levels of FDH transcript increase very quickly in response to stress, protein accumulation is much slower”
t0“Potato”
Claim · model-written summary
FDH protein in leaves can eventually reach levels found in tubers.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- leavese0
- Outcome
- protein accumulatione0
- Direction
- reach the same levelse0
- Comparator
- tuberse0
Not reportedGene
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“protein accumulation is much slower, but can eventually reach the same levels in leaves as in tubers.”
t0“Potato”
The same questions in other papers
application · molecular: expression · Solanum tuberosum (potato)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.