Abstract
In tobacco and Nicotiana benthamiana, limited cross-protection against cucumber mosaic virus strain Fny (Fny-CMV) was provided by prior inoculation with a deletion mutant lacking the 2b silencing-suppressor protein gene (Fny-CMVDelta2b). Cross-protection by Fny-CMVDelta2b did not result from induction of systemic RNA silencing. We investigated whether protection occurs through induction of localized RNA silencing by using Arabidopsis thaliana plants harbouring mutations in genes encoding the dicer-like (DCL) endoribonucleases 2, 3 and 4 involved in antiviral silencing. In wild-type A. thaliana (Col-0) plants, Fny-CMVDelta2b was symptomless and cross-protected against Fny-CMV infection. Cross-protection by Fny-CMVDelta2b against Fny-CMV infection was not abolished in dcl2, dcl3 or dcl4 mutant plants and was strongest in dcl2/4 double mutants, although in these plants and in dcl4 mutants, Fny-CMVDelta2b replicated to high levels and induced strong symptoms. The results suggest that Fny-CMVDelta2b/Fny-CMV cross-protection is not completely dependent on RNA silencing and also involves competition between these viruses.
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Claims 9
Claim · model-written summary
Prior Fny-CMVDelta2b inoculation gave limited protection in tobacco and Nicotiana benthamiana.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- prior inoculation with a deletion mutant lacking the 2b silencing-suppressor protein gene (Fny-CMVDelta2b)e0
- Interactor
- cucumber mosaic virus strain Fny
cucumber mosaic cucumoviruse0pathogen - Outcome
- cross-protection against cucumber mosaic virus strain Fnye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In tobacco and Nicotiana benthamiana, limited cross-protection against cucumber mosaic virus strain Fny (Fny-CMV) was provided by prior inoculation with a deletion mutant lacking the 2b silencing-suppressor protein gene (Fny-CMVDelta2b).”
Claim · model-written summary
Fny-CMVDelta2b cross-protection did not arise from systemic RNA silencing.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Interactor
- Fny-CMVDelta2be0pathogen
- Outcome
- Cross-protectione0
- Qualifier
- did not result from induction of systemic RNA silencinge0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“Cross-protection by Fny-CMVDelta2b did not result from induction of systemic RNA silencing.”
Claim · model-written summary
Fny-CMVDelta2b caused no symptoms in wild-type Arabidopsis.
- Taxon
- A. thalianaArabidopsis thalianae0
- Material
- Col-0e0
- Interactor
- Fny-CMVDelta2be0pathogen
- Outcome
- Fny-CMVDelta2b was symptomlesse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In wild-type A. thaliana (Col-0) plants, Fny-CMVDelta2b was symptomless”
Claim · model-written summary
Fny-CMVDelta2b protected wild-type Arabidopsis from Fny-CMV infection.
- Taxon
- A. thalianaArabidopsis thalianae0
- Material
- Col-0e0
- Treatment
- Fny-CMVDelta2be0
- Interactor
- Fny-CMVe0pathogen
- Outcome
- Fny-CMV infectione0
- Direction
- cross-protectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In wild-type A. thaliana (Col-0) plants, Fny-CMVDelta2b was symptomless and cross-protected against Fny-CMV infection.”
Claim · model-written summary
Protection persisted in dcl2, dcl3 and dcl4 mutants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- dcl2, dcl3 or dcl4 mutant plantse0
- Interactor
- Fny-CMVe0pathogen
- Outcome
- Cross-protection by Fny-CMVDelta2b against Fny-CMV infectione0
- Direction
- not abolishede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“Cross-protection by Fny-CMVDelta2b against Fny-CMV infection was not abolished in dcl2, dcl3 or dcl4 mutant plants”
Claim · model-written summary
Protection was strongest in dcl2/4 double mutants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- dcl2/4 double mutantse0
- Outcome
- strongeste0
- Direction
- strongeste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“was strongest in dcl2/4 double mutants”
Claim · model-written summary
Fny-CMVDelta2b replicated to high levels in dcl2/4 and dcl4 mutants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- dcl2/4 double mutantse1
- Interactor
- Fny-CMVDelta2be0pathogen
- Outcome
- Fny-CMVDelta2b replicated to high levelse0
- Direction
- high levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“in these plants and in dcl4 mutants, Fny-CMVDelta2b replicated to high levels”
e1“dcl2/4 double mutants”
Claim · model-written summary
Fny-CMVDelta2b caused strong symptoms in dcl2/4 and dcl4 mutants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- dcl4 mutantse0
- Interactor
- Fny-CMVDelta2be0pathogen
- Outcome
- induced strong symptomse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“in these plants and in dcl4 mutants, Fny-CMVDelta2b replicated to high levels and induced strong symptoms”
e1“dcl2/4 double mutants”
Claim · model-written summary
The authors infer that cross-protection also involves competition between the viruses.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- cross-protectione0
- Qualifier
- competition between these virusese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“cross-protection is not completely dependent on RNA silencing and also involves competition between these viruses.”
The same questions in other papers
loss of function · cucumber mosaic cucumovirus · Nicotiana tabacum (tobacco)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
inoculation · cucumber mosaic cucumovirus · Nicotiana tabacum (tobacco)Positive and negative results
4 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- negative
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.