Abstract
Studies on some plant species have shown that increasing the growth temperature gradually or pretreating with high temperature can lead to obvious photosynthetic acclimation to high temperature. To test whether this acclimation arises from heat adaptation of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco, EC 4.1.1.39) activation mediated by Rubisco activase (RCA), gene expression of RCA large isoform (RCA(L)) and RCA small isoform (RCA(S)) in rice was determined using a 4-day heat stress treatment [40/30 degrees C (day/night)] followed by a 3-day recovery under control conditions [30/22 degrees C (day/night)]. The heat stress significantly induced the expression of RCA(L) as determined by both mRNA and protein levels. Correlative analysis indicated that RCA(S) protein content was extremely significantly related to Rubisco initial activity and net photosynthetic rate (Pn) under both heat stress and normal conditions. Immunoblot analysis of the Rubisco-RCA complex revealed that the ratio of RCA(L) to Rubisco increased markedly in heat-acclimated rice leaves. Furthermore, transgenic rice plants expressing enhanced amounts of RCA(L) exhibited higher thermotolerance in Pn and Rubisco initial activity and grew better at high temperature than wild-type (WT) plants and transgenic rice plants expressing enhanced amounts of RCA(S). Under normal conditions, the transgenic rice plants expressing enhanced amounts of RCA(S) showed higher Pn and produced more biomass than transgenic rice plants expressing enhanced amounts of RCA(L) and wild-type plants. Together, these suggest that the heat-induced RCA(L) may play an important role in photosynthetic acclimation to moderate heat stress in vivo, while RCA(S) plays a major role in maintaining Rubisco initial activity under normal conditions.
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Claims 9
Claim · model-written summary
Heat stress significantly induced the expression of RCA(L) in rice, at both mRNA and protein levels.
- Taxon
- rice
Oryza sativat0 - Treatment
- heat stresse0
- Gene
- RCA(L)e0
- Outcome
- expression of RCA(L)e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The heat stress significantly induced the expression of RCA(L) as determined by both mRNA and protein levels”
t0“rice”
Claim · model-written summary
RCA(S) protein content was extremely significantly related to Rubisco initial activity and net photosynthetic rate under both heat stress and normal conditions.
- Taxon
- riceOryza sativat0
- Treatment
- heat stresse0
- Gene
- RCA(S)e0
- Outcome
- Rubisco initial activity and net photosynthetic rate (Pn)e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RCA(S) protein content was extremely significantly related to Rubisco initial activity and net photosynthetic rate (Pn) under both heat stress and normal conditions”
t0“rice”
Claim · model-written summary
The ratio of RCA(L) to Rubisco increased markedly in heat-acclimated rice leaves.
- Taxon
- riceOryza sativae0
- Organ / stage
- leavese0
- Treatment
- heat-acclimatede0
- Gene
- RCA(L)e0
- Outcome
- ratio of RCA(L) to Rubiscoe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ratio of RCA(L) to Rubisco increased markedly in heat-acclimated rice leaves”
Claim · model-written summary
Transgenic rice plants expressing enhanced RCA(L) showed higher thermotolerance in Pn and Rubisco initial activity than wild-type plants and RCA(S)-overexpressing transgenic plants.
- Taxon
- riceOryza sativae0
- Treatment
- expressing enhanced amounts of RCA(L)e0
- Gene
- RCA(L)e0
- Outcome
- thermotolerance in Pn and Rubisco initial activitye0
- Direction
- highere0
- Comparator
- wild-type (WT) plants and transgenic rice plants expressing enhanced amounts of RCA(S)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic rice plants expressing enhanced amounts of RCA(L) exhibited higher thermotolerance in Pn and Rubisco initial activity and grew better at high temperature than wild-type (WT) plants and transgenic rice plants expressing enhanced amounts of RCA(S)”
Claim · model-written summary
Transgenic rice plants expressing enhanced RCA(L) grew better at high temperature than wild-type plants and RCA(S)-overexpressing transgenic plants.
- Taxon
- riceOryza sativae0
- Treatment
- expressing enhanced amounts of RCA(L)e0
- Gene
- RCA(L)e0
- Outcome
- grewe0
- Direction
- bettere0
- Qualifier
- at high temperaturee0
- Comparator
- wild-type (WT) plants and transgenic rice plants expressing enhanced amounts of RCA(S)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic rice plants expressing enhanced amounts of RCA(L) exhibited higher thermotolerance in Pn and Rubisco initial activity and grew better at high temperature than wild-type (WT) plants and transgenic rice plants expressing enhanced amounts of RCA(S)”
Claim · model-written summary
Under normal conditions, transgenic rice plants expressing enhanced RCA(S) showed higher Pn than RCA(L)-overexpressing transgenic plants and wild-type plants.
- Taxon
- riceOryza sativae0
- Treatment
- expressing enhanced amounts of RCA(S)e0
- Gene
- RCA(S)e0
- Outcome
- Pne0
- Direction
- highere0
- Qualifier
- Under normal conditionse0
- Comparator
- transgenic rice plants expressing enhanced amounts of RCA(L) and wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under normal conditions, the transgenic rice plants expressing enhanced amounts of RCA(S) showed higher Pn and produced more biomass than transgenic rice plants expressing enhanced amounts of RCA(L) and wild-type plants”
Claim · model-written summary
Under normal conditions, transgenic rice plants expressing enhanced RCA(S) produced more biomass than RCA(L)-overexpressing transgenic plants and wild-type plants.
- Taxon
- riceOryza sativae0
- Treatment
- expressing enhanced amounts of RCA(S)e0
- Gene
- RCA(S)e0
- Outcome
- biomasse0
- Direction
- moree0
- Qualifier
- Under normal conditionse0
- Comparator
- transgenic rice plants expressing enhanced amounts of RCA(L) and wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under normal conditions, the transgenic rice plants expressing enhanced amounts of RCA(S) showed higher Pn and produced more biomass than transgenic rice plants expressing enhanced amounts of RCA(L) and wild-type plants”
Claim · model-written summary
The authors suggest heat-induced RCA(L) may play an important role in photosynthetic acclimation to moderate heat stress in vivo.
- Taxon
- riceOryza sativat0
- Treatment
- moderate heat stresse0
- Gene
- RCA(L)e0
- Outcome
- photosynthetic acclimatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the heat-induced RCA(L) may play an important role in photosynthetic acclimation to moderate heat stress in vivo”
t0“rice”
Claim · model-written summary
The authors suggest RCA(S) plays a major role in maintaining Rubisco initial activity under normal conditions.
- Taxon
- riceOryza sativat0
- Gene
- RCA(S)e0
- Outcome
- Rubisco initial activitye0
- Qualifier
- under normal conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RCA(S) plays a major role in maintaining Rubisco initial activity under normal conditions”
t0“rice”
The same questions in other papers
heat · physiology: photosynthesis · Oryza sativa (rice)Results differ
12 papers in the same direction as this paper · 34 in the opposite direction
- negative
- no effect
- no effect
43 papers more in this group
See all 47 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.