Abstract
The effect of 24-epibrassinolide (BR27) on cold resistance of rape seedlings was studied by ion leakage and photosynthetic pigment degradation measurements. Aqueous solutions of BR27 were injected into cotyledons or primary leaves of rape plants and these plants were incubated at 2 °C or 20 °C. Cold treatment (2 °C) without BR27 injection elevated the membrane permeability in both primary leaves and cotyledons significantly. Surprisingly, injection of leaves with water or 0.467 % aqueous ethanol solution led to a massive increase in membrane permeability after cold stress at 2 °C. The synergistic effect of leaf infiltration and cold on permeability was abolished by 0.05 and 1.00 µM of BR27 in primary leaves and by 1.00 µM of BR27 in cotyledons. On the other hand, BR27 solutions strongly elevated the membrane permeability at 20 °C, while water and ethanol solutions brought about only negligible increases. Water or ethanol infiltrations strongly reduced the leaf contents of chlorophyll (Chl) a, Chl b and carotenoids at 2 °C but less markedly at 20 °C. However, in seedlings exposed to 2 °C pigments content was significantly higher in BR27-treated leaves as compared to water/ethanol control. There were no differences between pigment contents of leaves injected with BR27 solutions or only water/ethanol at 20 °C. The above data strongly support the stress protecting effect of BR27.
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Claims 8
Claim · model-written summary
Cold at 2 °C without BR27 injection significantly elevated membrane permeability in primary leaves and cotyledons.
- Taxon
- rape
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Organ / stage
- primary leaves and cotyledonse1
- Treatment
- Cold treatment (2 °C)e1
- Outcome
- membrane permeabilitye1
- Direction
- elevatede1
- Comparator
- without BR27 injectione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
t0“rape leaves”
e1“Cold treatment (2 °C) without BR27 injection elevated the membrane permeability in both primary leaves and cotyledons significantly”
Claim · model-written summary
Water or ethanol infiltration of leaves under cold stress at 2 °C caused a massive increase in membrane permeability.
- Taxon
- rapeBrassica napust0
- Organ / stage
- leavese2
- Treatment
- injection of leaves with water or 0.467 % aqueous ethanol solutione2
- Outcome
- membrane permeabilitye2
- Direction
- increasee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rape leaves”
e2“Surprisingly, injection of leaves with water or 0.467 % aqueous ethanol solution led to a massive increase in membrane permeability after cold stress at 2 °C”
Claim · model-written summary
BR27 at 0.05 and 1.00 µM abolished the synergistic effect of leaf infiltration and cold on membrane permeability in primary leaves.
- Taxon
- rapeBrassica napust0
- Organ / stage
- primary leavese3
- Treatment
- 0.05 and 1.00 µM of BR27e3
- Outcome
- synergistic effect of leaf infiltration and cold on permeabilitye3
- Direction
- abolishede3
- Comparator
- leaf infiltration and colde3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rape leaves”
e3“The synergistic effect of leaf infiltration and cold on permeability was abolished by 0.05 and 1.00 µM of BR27 in primary leaves and by 1.00 µM of BR27 in cotyledons”
Claim · model-written summary
BR27 at 1.00 µM abolished the synergistic effect of leaf infiltration and cold on membrane permeability in cotyledons.
- Taxon
- rapeBrassica napust0
- Organ / stage
- cotyledonse3
- Treatment
- 1.00 µM of BR27e3
- Outcome
- synergistic effect of leaf infiltration and cold on permeabilitye3
- Direction
- abolishede3
- Comparator
- leaf infiltration and colde3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rape leaves”
e3“The synergistic effect of leaf infiltration and cold on permeability was abolished by 0.05 and 1.00 µM of BR27 in primary leaves and by 1.00 µM of BR27 in cotyledons”
Claim · model-written summary
BR27 solutions strongly elevated membrane permeability at 20 °C, whereas water and ethanol solutions caused only negligible increases.
- Taxon
- rapeBrassica napust0
- Treatment
- BR27 solutionse5
- Outcome
- membrane permeabilitye5
- Direction
- strongly elevatede5
- Qualifier
- at 20 °Ce5
- Comparator
- water and ethanol solutionse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rape leaves”
e5“On the other hand, BR27 solutions strongly elevated the membrane permeability at 20 °C, while water and ethanol solutions brought about only negligible increases”
Claim · model-written summary
Water or ethanol infiltration strongly reduced leaf chlorophyll a, chlorophyll b and carotenoid contents at 2 °C.
- Taxon
- rapeBrassica napust0
- Organ / stage
- leafe6
- Treatment
- Water or ethanol infiltrationse6
- Outcome
- leaf contents of chlorophyll (Chl) a, Chl b and carotenoidse6
- Direction
- strongly reducede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rape leaves”
e6“Water or ethanol infiltrations strongly reduced the leaf contents of chlorophyll (Chl) a, Chl b and carotenoids at 2 °C but less markedly at 20 °C”
Claim · model-written summary
At 2 °C, BR27-treated leaves had significantly higher pigment contents than water/ethanol controls.
- Taxon
- rapeBrassica napust0
- Treatment
- BR27-treated leavese7
- Outcome
- pigments contente7
- Direction
- highere7
- Qualifier
- exposed to 2 °Ce7
- Comparator
- water/ethanol controle7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rape leaves”
e7“in seedlings exposed to 2 °C pigments content was significantly higher in BR27-treated leaves as compared to water/ethanol control”
Claim · model-written summary
At 20 °C, pigment contents did not differ between BR27-injected and water/ethanol-injected leaves.
- Taxon
- rapeBrassica napust0
- Organ / stage
- leavese7
- Treatment
- BR27 solutionse7
- Outcome
- pigment contentse7
- Direction
- no differencese7
- Comparator
- only water/ethanole7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rape leaves”
e7“There were no differences between pigment contents of leaves injected with BR27 solutions or only water/ethanol at 20 °C”
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See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.