Abstract
Adenylate energy charge (AEC) and adenine nucleotide levels of isolated winter wheat (Triticum aestivum L. cv Kharkov 22 MC) cells exposed to various low temperature stresses were determined. During ice encasement at -1 degrees C, nucleotide levels decreased gradually in approximate relation to a decline in cell viability. AEC values remained high even after 5 weeks of icing when cell viability was severely reduced. When isolated cell suspensions were exposed to various cooling and freezing regimes ranging from -10 to -30 degrees C, cell damage was dependent on the minimum temperature imposed and the duration of exposure to the freezing stress. The levels of all three adenine nucleotides declined with increasing severity of the imposed stress, but AEC values remained high even at -30 degrees C when nearly all of the cells were killed. The addition of 10 millimolar Ca(2+) to cell suspensions enhanced survival during low temperature stresses, but did not influence nucleotide levels other than through its effect on cell viability. These results indicate that impairment of the ion transport system during the early stages of ice encasement prior to a detectable decline in cell viability cannot be attributed to changes in the adenylate energy charge system of the cell.
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Claims 9
Claim · model-written summary
Under ice encasement, wheat cell nucleotide levels and viability declined.
- Taxon
- winter wheat
Triticum aestivume1 - Material
- Kharkov 22 MCe1
- Treatment
- ice encasement at -1 degrees Ce0
- Outcome
- nucleotide levelse0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During ice encasement at -1 degrees C, nucleotide levels decreased gradually in approximate relation to a decline in cell viability”
e1“isolated winter wheat (Triticum aestivum L. cv Kharkov 22 MC) cells”
Claim · model-written summary
Wheat cell viability declined during ice encasement.
- Taxon
- winter wheatTriticum aestivume1
- Treatment
- ice encasement at -1 degrees Ce0
- Outcome
- cell viabilitye0
- Direction
- declinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During ice encasement at -1 degrees C, nucleotide levels decreased gradually in approximate relation to a decline in cell viability”
e1“isolated winter wheat (Triticum aestivum L. cv Kharkov 22 MC) cells”
Claim · model-written summary
AEC remained high after five weeks of icing despite severely reduced viability.
- Taxon
- winter wheatTriticum aestivume1
- Treatment
- icinge0
- Outcome
- AEC valuese0
- Direction
- remained highe0
- Qualifier
- even after 5 weeks of icing when cell viability was severely reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AEC values remained high even after 5 weeks of icing when cell viability was severely reduced”
e1“isolated winter wheat (Triticum aestivum L. cv Kharkov 22 MC) cells”
Claim · model-written summary
Wheat cell damage depended on minimum temperature and freezing duration.
- Taxon
- winter wheatTriticum aestivume1
- Treatment
- freezing stresse0
- Outcome
- cell damagee0
- Qualifier
- minimum temperature imposed and the duration of exposuree0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cell damage was dependent on the minimum temperature imposed and the duration of exposure to the freezing stress”
e1“isolated winter wheat (Triticum aestivum L. cv Kharkov 22 MC) cells”
Claim · model-written summary
All three nucleotide levels declined with increasing freezing severity.
- Taxon
- winter wheatTriticum aestivume1
- Treatment
- imposed stresse0
- Outcome
- levels of all three adenine nucleotidese0
- Direction
- declinede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The levels of all three adenine nucleotides declined with increasing severity of the imposed stress”
e1“isolated winter wheat (Triticum aestivum L. cv Kharkov 22 MC) cells”
Claim · model-written summary
AEC stayed high at -30 degrees C despite nearly complete cell death.
- Taxon
- winter wheatTriticum aestivume1
- Treatment
- -30 degrees Ce0
- Outcome
- AEC valuese0
- Direction
- remained highe0
- Qualifier
- nearly all of the cells were killede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AEC values remained high even at -30 degrees C when nearly all of the cells were killed”
e1“isolated winter wheat (Triticum aestivum L. cv Kharkov 22 MC) cells”
Claim · model-written summary
Adding calcium enhanced wheat cell survival under cold stress.
- Taxon
- winter wheatTriticum aestivume1
- Treatment
- 10 millimolar Ca(2+)e0
- Outcome
- survivale0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of 10 millimolar Ca(2+) to cell suspensions enhanced survival during low temperature stresses”
e1“isolated winter wheat (Triticum aestivum L. cv Kharkov 22 MC) cells”
Claim · model-written summary
Calcium did not influence nucleotide levels except through cell viability.
- Taxon
- winter wheatTriticum aestivume2
- Treatment
- 10 millimolar Ca(2+)e1
- Outcome
- nucleotide levelse0
- Direction
- did not influencee0
- Qualifier
- other than through its effect on cell viabilitye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“did not influence nucleotide levels other than through its effect on cell viability”
e1“The addition of 10 millimolar Ca(2+) to cell suspensions”
e2“isolated winter wheat (Triticum aestivum L. cv Kharkov 22 MC) cells”
Claim · model-written summary
Authors conclude early ion transport impairment under ice cannot be attributed to adenylate energy charge changes.
- Taxon
- winter wheatTriticum aestivume1
- Outcome
- impairment of the ion transport systeme0
- Qualifier
- cannot be attributed to changes in the adenylate energy charge system of the celle0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“impairment of the ion transport system during the early stages of ice encasement prior to a detectable decline in cell viability cannot be attributed to changes in the adenylate energy charge system of the cell”
e1“isolated winter wheat (Triticum aestivum L. cv Kharkov 22 MC) cells”
The same questions in other papers
calcium(2+) · molecular: metabolites · Triticum aestivum (wheat)Positive and negative results
2 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.