Abstract
The occurrence and roles of cGMP were investigated in aleurone layers and protoplasts isolated from barley (cv Himalaya) grain. Levels of cGMP in freshly isolated barley aleurone layers ranged from 0.065 to 0.08 pmol/g fresh weight of tissue, and cGMP levels increased transiently after incubation in gibberellic acid (GA). Abscisic acid (ABA) did not increase cGMP levels in aleurone layers. LY 83583 (LY), an inhibitor of guanylyl cyclase, prevented the GA-induced increase in cGMP and inhibited GA-induced [alpha]-amylase synthesis and secretion. The inhibitory effects of LY could be overcome by membrane-permeant analogs of cGMP. LY also prevented GA-induced accumulation of [alpha]-amylase and GAMYB mRNAs. cGMP alone was not sufficient to induce the accumulation of [alpha]-amylase or GAMYB mRNA. LY had a less dramatic effect on the accumulation of mRNAs encoding the ABA-responsive gene Rab21. We conclude that cGMP plays an important role in GA, but not ABA, signaling in the barley aleurone cell.
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Claims 10
Claim · model-written summary
Fresh barley aleurone layers contained 0.065 to 0.08 pmol/g fresh weight of cGMP.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Organ / stage
- aleurone layerse0
- Outcome
- Levels of cGMPe0
- Value
- 0.065 to 0.08 pmol/g fresh weight of tissuee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Levels of cGMP in freshly isolated barley aleurone layers ranged from 0.065 to 0.08 pmol/g fresh weight of tissue”
Claim · model-written summary
Gibberellic acid transiently increased cGMP levels in barley aleurone.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- gibberellic acid (GA)e0
- Outcome
- cGMP levelse0
- Direction
- increasede0
- Qualifier
- transientlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cGMP levels increased transiently after incubation in gibberellic acid (GA)”
t0“Barley Aleurone”
Claim · model-written summary
Abscisic acid did not increase cGMP in barley aleurone.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- Abscisic acid (ABA)e0
- Outcome
- cGMP levelse0
- Direction
- did not increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Abscisic acid (ABA) did not increase cGMP levels in aleurone layers”
t0“Barley Aleurone”
Claim · model-written summary
LY 83583 prevented the GA-induced increase in cGMP.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- LY 83583 (LY)e0
- Outcome
- GA-induced increase in cGMPe0
- Direction
- preventede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LY 83583 (LY), an inhibitor of guanylyl cyclase, prevented the GA-induced increase in cGMP”
t0“Barley Aleurone”
Claim · model-written summary
LY 83583 inhibited GA-induced alpha-amylase synthesis and secretion.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- LY 83583 (LY)e1
- Gene
- [alpha]-amylasee0
- Outcome
- [alpha]-amylase synthesis and secretione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibited GA-induced [alpha]-amylase synthesis and secretion”
t0“Barley Aleurone”
e1“LY 83583 (LY), an inhibitor of guanylyl cyclase”
Claim · model-written summary
Membrane-permeant cGMP analogs overcame LY inhibition.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- membrane-permeant analogs of cGMPe0
- Outcome
- inhibitory effects of LYe0
- Direction
- overcomee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The inhibitory effects of LY could be overcome by membrane-permeant analogs of cGMP”
t0“Barley Aleurone”
Claim · model-written summary
LY prevented GA-induced accumulation of alpha-amylase and GAMYB mRNAs.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- LYe0
- Gene
- [alpha]-amylase and GAMYBe0
- Outcome
- GA-induced accumulation of [alpha]-amylase and GAMYB mRNAse0
- Direction
- preventede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LY also prevented GA-induced accumulation of [alpha]-amylase and GAMYB mRNAs”
t0“Barley Aleurone”
Claim · model-written summary
cGMP alone did not induce alpha-amylase or GAMYB mRNA accumulation.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- cGMP alonee0
- Gene
- [alpha]-amylase or GAMYBe0
- Outcome
- accumulation of [alpha]-amylase or GAMYB mRNAe0
- Direction
- not sufficient to inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cGMP alone was not sufficient to induce the accumulation of [alpha]-amylase or GAMYB mRNA”
t0“Barley Aleurone”
Claim · model-written summary
LY had a less dramatic effect on Rab21 mRNA accumulation.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- LYe0
- Gene
- Rab21e0
- Outcome
- accumulation of mRNAs encoding the ABA-responsive gene Rab21e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LY had a less dramatic effect on the accumulation of mRNAs encoding the ABA-responsive gene Rab21”
t0“Barley Aleurone”
Claim · model-written summary
Authors conclude cGMP has a role in GA but not ABA signaling.
- Taxon
- barleyHordeum vulgaree0
- Compound
- cGMPe0
- Outcome
- GA, but not ABA, signalinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cGMP plays an important role in GA, but not ABA, signaling in the barley aleurone cell”
The same questions in other papers
(+)-abscisic acid · molecular: metabolites · Hordeum vulgare (barley)Positive and negative results
6 papers in the same direction as this paper · 8 in the opposite direction
- negative
- positive
- positive
11 papers more in this group
See all 15 papers →3',5'-cyclic GMP · molecular: expression · Hordeum vulgare (barley)Results agree
1 paper in the same direction as this paper
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.