Abstract
Thinopyrum intermedium carries many useful traits for wheat genetic improvement. To identify genes conferring resistance to stripe rust caused by Puccinia striiformis f. sp. tritici in Zhong4, one of the Zhong series of partial amphiploids from the hybrids of common wheat × Th. intermedium, a cross was made between Zhong4 and a Chinese Spring ph1b mutant (CS-ph1b). The parents, F 1 , F 2 plants and F 5 lines of the cross were tested with Chinese predominant race CYR31 of P. striiformis f. sp. tritici in the seedling stage under controlled greenhouse conditions. Of 201 F 2 seedlings tested with race CYR31, 149 were resistant and 52 were susceptible. The segregation fit a 3 resistant:1 susceptible ratio, indicating that a single dominant gene conferred resistance to the race CYR31. This result was further confirmed by the segregation of F 5 lines into 1:1 ratio (P = 0.77). Using the Schiff dyeing method, the chromosome number in the root-tip cells of 10 F 5 resistant lines (total 42 plants) was determined. Two alien addition lines (5-1-2-1-1-5, 5-1-2-1-1-6) with the smallest chromosome number (2n = 42 + II) were identified. A total of 105 pollen mother cells were examined at metaphase 1 (PMCs MI) for meiotic chromosome pairing. The lines 5-1-2-1-1-5 and 5-1-2-1-1-6 showed regular meiosis, exhibiting 22 ring or rod bivalents (2n = 22 II). The GISH results indicated that lines 5-1-2-1-1-5 and 5-1-2-1-1-6 were wheat-Th. intermedium alien disomic addition lines with 21 pairs of wheat chromosomes and one pair of Th. intermedium chromosomes. These two lines were all resistant to stripe rust, suggesting that they can be used as donors of stripe rust resistance genes and have great potential to play important roles in wheat stripe rust resistance breeding programs in China and the world.Key words: Wheat-Thinopyrum intermedium, stripe rust, GISH, cytological identification
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Claims 9
Claim · model-written summary
Seedling resistance to stripe rust segregated 149 resistant to 52 susceptible among 201 F2 seedlings.
- Taxon
- wheat
Triticum aestivumt0 - Material
- F 2 seedlingse0
- Interactor
- race CYR31e0pathogen
- Outcome
- resistante0
- Value
- 149e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Of 201 F 2 seedlings tested with race CYR31, 149 were resistant and 52 were susceptible”
t0“wheat-Thinopyrum intermedium”
Claim · model-written summary
The 3:1 segregation indicated one dominant gene conferring CYR31 resistance.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- race CYR31e0pathogen
- Gene
- single dominant genee0
- Outcome
- resistance to the race CYR31e0
- Value
- 3 resistant:1 susceptiblee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The segregation fit a 3 resistant:1 susceptible ratio, indicating that a single dominant gene conferred resistance to the race CYR31”
t0“wheat-Thinopyrum intermedium”
Claim · model-written summary
F5 lines segregated 1:1, supporting single-gene resistance.
- Taxon
- wheatTriticum aestivumt0
- Material
- F 5 linese0
- Outcome
- segregatione0
- Value
- 1:1 ratioe0
- Qualifier
- P = 0.77e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“segregation of F 5 lines into 1:1 ratio (P = 0.77)”
t0“wheat-Thinopyrum intermedium”
Claim · model-written summary
5-1-2-1-1-5 was identified as an alien addition line with 2n = 42 + II.
- Taxon
- wheatTriticum aestivumt0
- Material
- 5-1-2-1-1-5e0
- Outcome
- alien addition linese0
- Value
- 2n = 42 + IIe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two alien addition lines (5-1-2-1-1-5, 5-1-2-1-1-6) with the smallest chromosome number (2n = 42 + II) were identified”
t0“wheat-Thinopyrum intermedium”
Claim · model-written summary
5-1-2-1-1-6 was identified as an alien addition line with 2n = 42 + II.
- Taxon
- wheatTriticum aestivumt0
- Material
- 5-1-2-1-1-6e0
- Outcome
- alien addition linese0
- Value
- 2n = 42 + IIe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two alien addition lines (5-1-2-1-1-5, 5-1-2-1-1-6) with the smallest chromosome number (2n = 42 + II) were identified”
t0“wheat-Thinopyrum intermedium”
Claim · model-written summary
The two addition lines showed regular meiosis with 22 bivalents.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- regular meiosise0
- Value
- 22 ring or rod bivalentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The lines 5-1-2-1-1-5 and 5-1-2-1-1-6 showed regular meiosis, exhibiting 22 ring or rod bivalents (2n = 22 II)”
t0“wheat-Thinopyrum intermedium”
Claim · model-written summary
GISH identified both lines as wheat-Th. intermedium disomic additions with 21 wheat chromosome pairs and one Th. intermedium pair.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- wheat-Th. intermedium alien disomic addition linese0
- Value
- 21 pairs of wheat chromosomes and one pair of Th. intermedium chromosomese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lines 5-1-2-1-1-5 and 5-1-2-1-1-6 were wheat-Th. intermedium alien disomic addition lines with 21 pairs of wheat chromosomes and one pair of Th. intermedium chromosomes”
t0“wheat-Thinopyrum intermedium”
Claim · model-written summary
Both addition lines resisted stripe rust.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- stripe ruste0pathogen
- Outcome
- resistant to stripe ruste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These two lines were all resistant to stripe rust”
t0“wheat-Thinopyrum intermedium”
Claim · model-written summary
The authors propose both lines as stripe rust resistance donors for wheat breeding.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- donors of stripe rust resistance genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“they can be used as donors of stripe rust resistance genes and have great potential to play important roles in wheat stripe rust resistance breeding programs”
t0“wheat-Thinopyrum intermedium”