Abstract
BACKGROUND: The hormone auxin plays an important role not only in the growth and development of rice, but also in its defense responses. We've previously shown that the P450 gene CYP71Z2 enhances disease resistance to pathogens through regulation of phytoalexin biosynthesis in rice, though it remains unclear if auxin is involved in this process or not. METHODOLOGY AND PRINCIPAL FINDINGS: The expression of CYP71Z2 was induced by Xanthomonas oryzae pv. oryzae (Xoo) inoculation was analyzed by qRT-PCR, with GUS histochemical staining showing that CYP71Z2 expression was limited to roots, blades and nodes. Overexpression of CYP71Z2 in rice durably and stably increased resistance to Xoo, though no significant difference in disease resistance was detected between CYP71Z2-RNA interference (RNAi) rice and wild-type. Moreover, IAA concentration was determined using the HPLC/electrospray ionization/tandem mass spectrometry system. The accumulation of IAA was significantly reduced in CYP71Z2-overexpressing rice regardless of whether plants were inoculated or not, whereas it was unaffected in CYP71Z2-RNAi rice. Furthermore, the expression of genes related to IAA, expansin and SA/JA signaling pathways was suppressed in CYP71Z2-overexpressing rice with or without inoculation. CONCLUSIONS AND SIGNIFICANCE: These results suggest that CYP71Z2-mediated resistance to Xoo may be via suppression of IAA signaling in rice. Our studies also provide comprehensive insight into molecular mechanism of resistance to Xoo mediated by IAA in rice. Moreover, an available approach for understanding the P450 gene functions in interaction between rice and pathogens has been provided.
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Claims 8
Claim · model-written summary
Xoo inoculation induced CYP71Z2 expression in rice.
- Taxon
- Rice
Oryza sativat0 - Treatment
- Xanthomonas oryzae pv. oryzae (Xoo) inoculatione0
- Interactor
- Xanthomonas oryzae pv. oryzae
Xanthomonas oryzae pv. oryzaee0pathogen - Gene
- CYP71Z2e0
- Outcome
- expression of CYP71Z2e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of CYP71Z2 was induced by Xanthomonas oryzae pv. oryzae (Xoo) inoculation”
t0“Rice”
Claim · model-written summary
CYP71Z2 expression was confined to rice roots, blades and nodes.
- Taxon
- RiceOryza sativat0
- Organ / stage
- roots, blades and nodese0
- Gene
- CYP71Z2e0
- Outcome
- CYP71Z2 expression was limited to roots, blades and nodese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CYP71Z2 expression was limited to roots, blades and nodes”
t0“Rice”
Claim · model-written summary
CYP71Z2 overexpression durably increased rice resistance to Xoo.
- Taxon
- riceOryza sativae0
- Treatment
- Overexpression of CYP71Z2e0
- Interactor
- Xanthomonas oryzae pv. oryzaeXanthomonas oryzae pv. oryzaee1pathogen
- Gene
- CYP71Z2e0
- Outcome
- resistance to Xooe0
- Direction
- increasede0
- Qualifier
- durably and stablye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of CYP71Z2 in rice durably and stably increased resistance to Xoo”
e1“Xanthomonas oryzae pv. oryzae (Xoo) inoculation”
Claim · model-written summary
CYP71Z2 RNA interference rice did not differ significantly from wild type in disease resistance.
- Taxon
- riceOryza sativae0
- Treatment
- CYP71Z2-RNA interference (RNAi) ricee0
- Interactor
- Xanthomonas oryzae pv. oryzaeXanthomonas oryzae pv. oryzaee1pathogen
- Gene
- CYP71Z2e0
- Outcome
- disease resistancee0
- Direction
- no significant differencee0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no significant difference in disease resistance was detected between CYP71Z2-RNA interference (RNAi) rice and wild-type”
e1“Xanthomonas oryzae pv. oryzae (Xoo) inoculation”
Claim · model-written summary
CYP71Z2 overexpression significantly reduced IAA accumulation in rice, with or without inoculation.
- Taxon
- riceOryza sativae0
- Treatment
- CYP71Z2-overexpressing ricee0
- Gene
- CYP71Z2e0
- Outcome
- accumulation of IAAe0
- Direction
- reducede0
- Qualifier
- regardless of whether plants were inoculated or note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The accumulation of IAA was significantly reduced in CYP71Z2-overexpressing rice regardless of whether plants were inoculated or not”
Claim · model-written summary
IAA accumulation was unaffected in CYP71Z2 RNA interference rice.
- Taxon
- riceOryza sativae0
- Treatment
- CYP71Z2-RNAi ricee0
- Gene
- CYP71Z2e0
- Outcome
- accumulation of IAAe0
- Direction
- unaffectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The accumulation of IAA was significantly reduced in CYP71Z2-overexpressing rice regardless of whether plants were inoculated or not, whereas it was unaffected in CYP71Z2-RNAi rice”
Claim · model-written summary
CYP71Z2 overexpression suppressed expression of IAA-, expansin- and SA/JA-related genes with or without inoculation.
- Taxon
- riceOryza sativae0
- Treatment
- CYP71Z2-overexpressing ricee0
- Gene
- genes related to IAA, expansin and SA/JA signaling pathwayse0
- Outcome
- expression of genes related to IAA, expansin and SA/JA signaling pathwayse0
- Direction
- suppressede0
- Qualifier
- with or without inoculatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of genes related to IAA, expansin and SA/JA signaling pathways was suppressed in CYP71Z2-overexpressing rice with or without inoculation”
Claim · model-written summary
The authors suggest that CYP71Z2-mediated resistance to Xoo may involve suppression of IAA signaling.
- Taxon
- riceOryza sativae0
- Interactor
- Xooe0pathogen
- Gene
- CYP71Z2e0
- Outcome
- resistance to Xooe0
- Qualifier
- suppression of IAA signalinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CYP71Z2-mediated resistance to Xoo may be via suppression of IAA signaling in rice”
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