Abstract
Tobacco (Nicotiana tabacum) pith explants were grown on manganese containing medium. At moderate concentration (10 millimolar), manganese selectively inhibited chlorophyll synthesis, resulting initially in growth of white callus. Several weeks later the white callus turned brown due to the accumulation of a pigment identified as protoporphyrin IX by its elution profile using high performance liquid chromatography, by its absorption spectrum, and by its fluorescence properties. At a concentration of 100 millimolar manganese the pigment accumulated without growth of the explant.
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Claims 4
Claim · model-written summary
At 10 millimolar manganese, tobacco callus chlorophyll synthesis was inhibited and white callus initially grew.
- Taxon
- Tobacco
Nicotiana tabacume0 - Treatment
- 10 millimolare0
- Outcome
- chlorophyll synthesise0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tobacco (Nicotiana tabacum) pith explants were grown on manganese containing medium. At moderate concentration (10 millimolar), manganese selectively inhibited chlorophyll synthesis, resulting initially in growth of white callus”
Claim · model-written summary
After several weeks the white tobacco callus became brown through pigment accumulation.
- Taxon
- TobaccoNicotiana tabacumt0
- Outcome
- white callus turned browne0
- Qualifier
- Several weeks latere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Several weeks later the white callus turned brown due to the accumulation of a pigment identified as protoporphyrin IX”
t0“Tobacco”
Claim · model-written summary
The pigment accumulating in tobacco callus was identified as protoporphyrin IX.
- Taxon
- TobaccoNicotiana tabacumt0
- Outcome
- a pigment identified as protoporphyrin IXe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a pigment identified as protoporphyrin IX by its elution profile using high performance liquid chromatography, by its absorption spectrum, and by its fluorescence properties”
t0“Tobacco”
Claim · model-written summary
At 100 millimolar manganese, pigment accumulated without explant growth.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- 100 millimolar manganesee0
- Outcome
- pigment accumulated without growth of the explante0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At a concentration of 100 millimolar manganese the pigment accumulated without growth of the explant”
t0“Tobacco”
The same questions in other papers
manganese atom · physiology: pigments · Nicotiana tabacum (tobacco)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.