Paper · 1971 · PLANT PHYSIOLOGY

Inhibition of Carotenoid Synthesis as a Mechanism of Action of Amitrole, Dichlormate, and Pyriclor

Earl R. Burns; Gale A. Buchanan; Mason C. Carter

10
claims
17
verbatim quotes
92
citations
1
research areas
✓
Open access

Abstract

Amitrole (3-amino-s-triazole), dichlormate (3,4-dichlorobenzyl methylcarbamate), and pyriclor (2,3,5-trichloro-4-pyridinol) inhibited normal carotenogenesis in etiolated wheat (Triticum aestivum L. var. Coker 65-20) seedlings. Carotenoid precursors accumulated in treated plants. In dichlormate-treated plants, zeta-carotene accumulated, whereas phytofluene, phytoene, and zeta-carotene accumulated in amitrole- and pyriclor-treated plants. None of the herbicides interfered with protochlorophyllide synthesis or its conversion to chlorophyllide when etiolated plants were illuminated. Chlorophyll accumulated in treated plants exposed to light at 60 foot candles, but was unstable and partially destroyed by illumination at 4000 foot candles. These data suggest that the phytotoxicity of amitrole, pyriclor, and dichlormate is due to inhibition of the synthesis of carotenoids and to the consequent photodestruction of chlorophyll and chloroplast disruption.

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Claims 10

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Amitrole inhibited normal carotenogenesis in etiolated wheat seedlings.

Material
Coker 65-20e0
Treatment
Amitrolee0
Outcome
normal carotenogenesise0
Direction
inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Amitrole (3-amino-s-triazole), dichlormate (3,4-dichlorobenzyl methylcarbamate), and pyriclor (2,3,5-trichloro-4-pyridinol) inhibited normal carotenogenesis in etiolated wheat (Triticum aestivum L. var. Coker 65-20) seedlings.”

✓ located verbatim in the source · abstract, characters 0–226 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Dichlormate inhibited normal carotenogenesis in etiolated wheat seedlings.

Material
Coker 65-20e0
Treatment
dichlormatee0
Outcome
normal carotenogenesise0
Direction
inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Amitrole (3-amino-s-triazole), dichlormate (3,4-dichlorobenzyl methylcarbamate), and pyriclor (2,3,5-trichloro-4-pyridinol) inhibited normal carotenogenesis in etiolated wheat (Triticum aestivum L. var. Coker 65-20) seedlings.”

✓ located verbatim in the source · abstract, characters 0–226 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Pyriclor inhibited normal carotenogenesis in etiolated wheat seedlings.

Material
Coker 65-20e0
Treatment
pyriclore0
Outcome
normal carotenogenesise0
Direction
inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Amitrole (3-amino-s-triazole), dichlormate (3,4-dichlorobenzyl methylcarbamate), and pyriclor (2,3,5-trichloro-4-pyridinol) inhibited normal carotenogenesis in etiolated wheat (Triticum aestivum L. var. Coker 65-20) seedlings.”

✓ located verbatim in the source · abstract, characters 0–226 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Zeta-carotene accumulated under dichlormate.

Treatment
dichlormatee0
Outcome
zeta-carotene accumulatede0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“In dichlormate-treated plants, zeta-carotene accumulated, whereas phytofluene, phytoene, and zeta-carotene accumulated in amitrole- and pyriclor-treated plants.”

✓ located verbatim in the source · abstract, characters 280–440 · show in text ↑
e1

“wheat (Triticum aestivum L. var. Coker 65-20) seedlings”

✓ located verbatim in the source · abstract, characters 170–225 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Phytofluene, phytoene, and zeta-carotene accumulated under amitrole.

Treatment
amitrolee0
Outcome
phytofluene, phytoene, and zeta-carotene accumulatede0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“In dichlormate-treated plants, zeta-carotene accumulated, whereas phytofluene, phytoene, and zeta-carotene accumulated in amitrole- and pyriclor-treated plants.”

✓ located verbatim in the source · abstract, characters 280–440 · show in text ↑
e1

“wheat (Triticum aestivum L. var. Coker 65-20) seedlings”

✓ located verbatim in the source · abstract, characters 170–225 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Phytofluene, phytoene, and zeta-carotene accumulated under pyriclor.

Treatment
pyriclore0
Outcome
phytofluene, phytoene, and zeta-carotene accumulatede0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“In dichlormate-treated plants, zeta-carotene accumulated, whereas phytofluene, phytoene, and zeta-carotene accumulated in amitrole- and pyriclor-treated plants.”

✓ located verbatim in the source · abstract, characters 280–440 · show in text ↑
e1

“wheat (Triticum aestivum L. var. Coker 65-20) seedlings”

✓ located verbatim in the source · abstract, characters 170–225 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

The three herbicides did not interfere with protochlorophyllide synthesis or conversion to chlorophyllide.

Treatment
herbicidese0
Outcome
protochlorophyllide synthesis or its conversion to chlorophyllidee0
Direction
no_changeinferrede0
Qualifier
when etiolated plants were illuminatede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“None of the herbicides interfered with protochlorophyllide synthesis or its conversion to chlorophyllide when etiolated plants were illuminated.”

✓ located verbatim in the source · abstract, characters 441–585 · show in text ↑
e1

“wheat (Triticum aestivum L. var. Coker 65-20) seedlings”

✓ located verbatim in the source · abstract, characters 170–225 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Chlorophyll accumulated in treated plants at 60 foot candles.

Treatment
light at 60 foot candlese0
Outcome
Chlorophyll accumulatede0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Chlorophyll accumulated in treated plants exposed to light at 60 foot candles, but was unstable and partially destroyed by illumination at 4000 foot candles.”

✓ located verbatim in the source · abstract, characters 586–743 · show in text ↑
e1

“wheat (Triticum aestivum L. var. Coker 65-20) seedlings”

✓ located verbatim in the source · abstract, characters 170–225 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Chlorophyll was partly destroyed at 4000 foot candles.

Treatment
illumination at 4000 foot candlese0
Outcome
Chlorophylle0
Direction
partially destroyede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Chlorophyll accumulated in treated plants exposed to light at 60 foot candles, but was unstable and partially destroyed by illumination at 4000 foot candles.”

✓ located verbatim in the source · abstract, characters 586–743 · show in text ↑
e1

“wheat (Triticum aestivum L. var. Coker 65-20) seedlings”

✓ located verbatim in the source · abstract, characters 170–225 · show in text ↑
Mechanismauthor interpretationoriginal resultnegativemeasured

Claim · model-written summary

Authors attributed phytotoxicity to carotenoid inhibition and subsequent chlorophyll photodestruction and chloroplast disruption.

Treatment
amitrole, pyriclor, and dichlormatee0
Outcome
phytotoxicitye0
Qualifier
inhibition of the synthesis of carotenoids and to the consequent photodestruction of chlorophyll and chloroplast disruptione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“These data suggest that the phytotoxicity of amitrole, pyriclor, and dichlormate is due to inhibition of the synthesis of carotenoids and to the consequent photodestruction of chlorophyll and chloroplast disruption.”

✓ located verbatim in the source · abstract, characters 744–959 · show in text ↑
e1

“wheat (Triticum aestivum L. var. Coker 65-20) seedlings”

✓ located verbatim in the source · abstract, characters 170–225 · show in text ↑

light · physiology: pigments · Triticum aestivum (wheat)Results differ

22 papers: 13 positive, 9 negative or no effect

8 papers in the same direction as this paper · 13 in the opposite direction

  1. positive
  2. positive
  3. positive

18 papers more in this group

See all 22 papers →

herbicide · molecular: metabolites · Triticum aestivum (wheat)Positive and negative results

22 papers: 16 positive, 6 negative or no effect

5 papers in the same direction as this paper · 16 in the opposite direction

  1. positive
  2. positive
  3. positive

18 papers more in this group

See all 22 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.