Abstract
During a 3-year study of the ectomycorrhizal fungi of subalpine forests in the Front Ranges of the Canadian Rockies, species of Russula and Lactarius were conspicuous mycobionts of both erect and krummholz forms of Abies lasiocarpa (Hook.) Nutt. and Picea engelmannii Parry. Morphological identifications of Russulaceous mycorrhizae were confirmed by comparing polymerase chain reaction amplified ribosomal DNA (internal transcribed spacer region) with that of sporocarp tissue. Restriction fragment length polymorphism analysis using AluI, HhaI, HinfI, and RsaI gave a distinctive profile for each of 14 Russulaceous sporocarps and facilitated the identification of five mycorrhizae. Mantles formed by Lactarii (Lactarius alnicola, Lactarins caespitosus, and Lactarius deliciosus var. areolatus) exhibit characteristic laticifers and pigments comparable to the associated sporocarp. Those formed by species of Russula (R. brevipes and R. silvicola) bear distinctive cystidia or sulphovanillin-reactive cells. Key words: ITS, Lactarius, RFLP, Russula, subalpine, tree line.
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Claims 5
Claim · model-written summary
RFLP analysis gave a distinctive profile for each of 14 Russulaceous sporocarps
- Taxon
- Abies lasiocarpa and Picea engelmannii
Picea engelmannii
Abies lasiocarpat0 - Interactor
- Russulaceouse2symbiont
- Outcome
- distinctive profilee2
- Value
- 14 Russulaceous sporocarpse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Russulaceous ectomycorrhizae of Abies lasiocarpa and Picea engelmannii”
e2“Restriction fragment length polymorphism analysis using AluI, HhaI, HinfI, and RsaI gave a distinctive profile for each of 14 Russulaceous sporocarps and facilitated the identification of five mycorrhizae”
Claim · model-written summary
RFLP analysis facilitated the identification of five mycorrhizae of Russulaceous fungi
- Taxon
- Abies lasiocarpa and Picea engelmanniiPicea engelmanniiAbies lasiocarpat0
- Interactor
- Russulaceouse2symbiont
- Outcome
- identification of five mycorrhizaee2
- Value
- fivee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Russulaceous ectomycorrhizae of Abies lasiocarpa and Picea engelmannii”
e2“Restriction fragment length polymorphism analysis using AluI, HhaI, HinfI, and RsaI gave a distinctive profile for each of 14 Russulaceous sporocarps and facilitated the identification of five mycorrhizae”
Claim · model-written summary
Morphological identifications of Russulaceous mycorrhizae were confirmed by PCR-amplified ITS comparison with sporocarp tissue
- Taxon
- Abies lasiocarpa and Picea engelmanniiPicea engelmanniiAbies lasiocarpat0
- Outcome
- Morphological identificationse3
- Comparator
- sporocarp tissuee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Russulaceous ectomycorrhizae of Abies lasiocarpa and Picea engelmannii”
e3“Morphological identifications of Russulaceous mycorrhizae were confirmed by comparing polymerase chain reaction amplified ribosomal DNA (internal transcribed spacer region) with that of sporocarp tissue”
Claim · model-written summary
Lactarius mantles exhibit characteristic laticifers and pigments comparable to the associated sporocarp
- Taxon
- Abies lasiocarpa and Picea engelmanniiPicea engelmanniiAbies lasiocarpat0
- Organ / stage
- Mantlese4
- Interactor
- Lactariie4symbiont
- Outcome
- characteristic laticifers and pigmentse4
- Comparator
- the associated sporocarpe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Russulaceous ectomycorrhizae of Abies lasiocarpa and Picea engelmannii”
e4“Mantles formed by Lactarii (Lactarius alnicola, Lactarins caespitosus, and Lactarius deliciosus var. areolatus) exhibit characteristic laticifers and pigments comparable to the associated sporocarp”
Claim · model-written summary
Russula mantles bear distinctive cystidia or sulphovanillin-reactive cells
- Taxon
- Abies lasiocarpa and Picea engelmanniiPicea engelmanniiAbies lasiocarpat0
- Outcome
- distinctive cystidia or sulphovanillin-reactive cellse5
RussulaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Russulaceous ectomycorrhizae of Abies lasiocarpa and Picea engelmannii”
e5“Those formed by species of Russula (R. brevipes and R. silvicola) bear distinctive cystidia or sulphovanillin-reactive cells”