Abstract
In different plant species, secondary metabolite biosynthesis is regulated by the phytohormone jasmonic acid (JA), which is derived by the action of lipoxygenase. In this study, we examined mono- and sesquiterpenoid accumulation and the related signal transduction pathways and biosynthetic genes in adventitious root cultures of Panax ginseng C.A. Meyer as induced by yeast extract (YE, 3 g/L), a biotic elicitor, and salicylic acid (SA, 200 μM), a signaling elicitor. The lipoxygenase ( LOX ) gene was highly expressed in 24 and 12 h after treatment with SA and YE. JA content was significantly increased in 24 h after SA treatment. The H 2 O 2 content was the highest in 24 and 72 h after the onset of SA and YE treatment, respectively. RNA blot analysis showed that farnesyl diphosphate synthase ( FPS ) and isopentenyl pyrophosphate isomerase ( IPPI ) genes encoding enzymes of the biosynthesis of mono- and sesquiterpenoids were up-regulated by both elicitors. Farensol, isochiapin B sesquiterpenoids, champhor, and cineole monoterpenoids were highly accumulated after 24 h of SA treatment, while YE treatment induced bacchotricuneatin C, guaiazulene, isochiapin B, and p-benzoquinone sesquiterpenoid production. These results suggest that mono- and sesquiterpenoid accumulation induced by SA and YE occurs due to the IPPI and FPS expression and may be mediated by reactive oxygen species signaling and jasmonic acid signal transduction.
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Claims 10
Claim · model-written summary
Salicylic acid induced high LOX expression after 24 h in ginseng roots.
- Taxon
- Panax ginseng C.A. Meyer
Panax ginsenge1 - Treatment
- SAe0
- Gene
- LOXe0
- Outcome
- LOX ) gene was highly expressede0
- Direction
- highly expressede0
- Qualifier
- 24e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The lipoxygenase ( LOX ) gene was highly expressed in 24 and 12 h after treatment with SA and YE.”
e1“adventitious root cultures of Panax ginseng C.A. Meyer as induced by yeast extract (YE, 3 g/L), a biotic elicitor, and salicylic acid (SA, 200 μM)”
Claim · model-written summary
Yeast extract induced high LOX expression after 12 h.
- Taxon
- Panax ginseng C.A. MeyerPanax ginsenge1
- Treatment
- YEe0
- Gene
- LOXe0
- Outcome
- LOX ) gene was highly expressede0
- Direction
- highly expressede0
- Qualifier
- 12 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The lipoxygenase ( LOX ) gene was highly expressed in 24 and 12 h after treatment with SA and YE.”
e1“adventitious root cultures of Panax ginseng C.A. Meyer”
Claim · model-written summary
Salicylic acid significantly increased JA content after 24 h.
- Taxon
- Panax ginseng C.A. MeyerPanax ginsenge1
- Treatment
- SAe0
- Outcome
- JA contente0
- Direction
- increasede0
- Qualifier
- 24 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JA content was significantly increased in 24 h after SA treatment.”
e1“adventitious root cultures of Panax ginseng C.A. Meyer”
Claim · model-written summary
H2O2 content peaked 24 h after salicylic acid treatment.
- Taxon
- Panax ginseng C.A. MeyerPanax ginsenge1
- Treatment
- SAe0
- Outcome
- H 2 O 2 contente0
- Direction
- higheste0
- Qualifier
- 24e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The H 2 O 2 content was the highest in 24 and 72 h after the onset of SA and YE treatment, respectively.”
e1“adventitious root cultures of Panax ginseng C.A. Meyer”
Claim · model-written summary
H2O2 content peaked 72 h after yeast extract treatment.
- Taxon
- Panax ginseng C.A. MeyerPanax ginsenge1
- Treatment
- YEe0
- Outcome
- H 2 O 2 contente0
- Direction
- higheste0
- Qualifier
- 72 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The H 2 O 2 content was the highest in 24 and 72 h after the onset of SA and YE treatment, respectively.”
e1“adventitious root cultures of Panax ginseng C.A. Meyer”
Claim · model-written summary
Salicylic acid upregulated FPS and IPPI genes.
- Taxon
- Panax ginseng C.A. MeyerPanax ginsenge2
- Treatment
- SAe1
- Gene
- FPSe0
- Outcome
- FPS ) and isopentenyl pyrophosphate isomerase ( IPPI ) genese0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“farnesyl diphosphate synthase ( FPS ) and isopentenyl pyrophosphate isomerase ( IPPI ) genes encoding enzymes of the biosynthesis of mono- and sesquiterpenoids were up-regulated by both elicitors.”
e1“salicylic acid (SA, 200 μM)”
e2“adventitious root cultures of Panax ginseng C.A. Meyer”
Claim · model-written summary
Yeast extract upregulated FPS and IPPI genes.
- Taxon
- Panax ginseng C.A. MeyerPanax ginsenge2
- Treatment
- YEe1
- Gene
- FPSe0
- Outcome
- FPS ) and isopentenyl pyrophosphate isomerase ( IPPI ) genese0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“farnesyl diphosphate synthase ( FPS ) and isopentenyl pyrophosphate isomerase ( IPPI ) genes encoding enzymes of the biosynthesis of mono- and sesquiterpenoids were up-regulated by both elicitors.”
e1“yeast extract (YE, 3 g/L)”
e2“adventitious root cultures of Panax ginseng C.A. Meyer”
Claim · model-written summary
Salicylic acid caused high accumulation of farensol, isochiapin B, champhor and cineole after 24 h.
- Taxon
- Panax ginseng C.A. MeyerPanax ginsenge1
- Treatment
- SAe0
- Outcome
- Farensol, isochiapin B sesquiterpenoids, champhor, and cineole monoterpenoidse0
- Direction
- highly accumulatede0
- Qualifier
- 24 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Farensol, isochiapin B sesquiterpenoids, champhor, and cineole monoterpenoids were highly accumulated after 24 h of SA treatment”
e1“adventitious root cultures of Panax ginseng C.A. Meyer”
Claim · model-written summary
Yeast extract induced bacchotricuneatin C, guaiazulene, isochiapin B and p-benzoquinone production.
- Taxon
- Panax ginseng C.A. MeyerPanax ginsenge1
- Treatment
- YEe0
- Outcome
- bacchotricuneatin C, guaiazulene, isochiapin B, and p-benzoquinone sesquiterpenoid productione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“YE treatment induced bacchotricuneatin C, guaiazulene, isochiapin B, and p-benzoquinone sesquiterpenoid production.”
e1“adventitious root cultures of Panax ginseng C.A. Meyer”
Claim · model-written summary
The authors suggest IPPI and FPS expression, reactive oxygen species and jasmonic acid signaling mediate elicitor-induced terpenoid accumulation.
- Taxon
- Panax ginseng C.A. MeyerPanax ginsenge1
- Gene
- IPPI and FPSe0
- Outcome
- mono- and sesquiterpenoid accumulatione0
- Qualifier
- may be mediated by reactive oxygen species signaling and jasmonic acid signal transductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mono- and sesquiterpenoid accumulation induced by SA and YE occurs due to the IPPI and FPS expression and may be mediated by reactive oxygen species signaling and jasmonic acid signal transduction.”
e1“adventitious root cultures of Panax ginseng C.A. Meyer”
The same questions in other papers
salicylic acid · molecular: metabolites · Panax ginseng (ginseng)Results agree
1 paper in the same direction as this paper
- positive
salicylic acid · molecular: expression · Panax ginseng (ginseng)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.