Abstract
Numerous investigations have been carried out to determine the impact of genetically modified plants, such as potato, maize, alfalfa and tobacco, on soil microorganisms and the results are contradictory. We applied classical microbiology methods to study quantitative changes of bacterial and fungal abundance in substrate and rhizosphere from barley (Hordeum vulgare L.) transgenic line H228.2A containing Rpg1 and bar genes, and its parent nontransgenic variety 'Golden Promise'. In addition, molecular biology methods were used to determine, if horizontal gene transfer from barley transgenic line to soil bacteria has occurred under experimental conditions by screening bacterial genomes for the presence of Rpg1 gene sequences using polymerase chain reactions. Results did not show significant impact on substrate and rhizosphere microorganisms. In the second pot experiment the average number of filamentous fungi in the rhizosphere of parent line 'Golden Promise' was higher than in the rhizosphere of transgenic line, but the variation among samples was very high probably caused by the high variation of substrate moisture content. None of bacterial deoxyribonucleic acid (DNA) samples isolated from substrate of rhizosphere of barley transgenic line resulted in positive amplification of Rpg1 gene-specific primer product.
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Claims 3
Claim · model-written summary
The transgenic barley line showed no significant overall impact on substrate and rhizosphere microorganisms.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaret0 - Material
- H228.2Ae1
- Treatment
- transgenic line H228.2Ae1
- Outcome
- substrate and rhizosphere microorganismse0
- Comparator
- parent nontransgenic variety 'Golden Promise'e1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Results did not show significant impact on substrate and rhizosphere microorganisms.”
e1“barley (Hordeum vulgare L.) transgenic line H228.2A containing Rpg1 and bar genes, and its parent nontransgenic variety 'Golden Promise'”
t0“barley (Hordeum vulgare L.)”
Claim · model-written summary
Filamentous fungi were more abundant around parent barley than the transgenic line in the second pot experiment.
- Taxon
- barleyHordeum vulgaret0
- Material
- 'Golden Promise'e0
- Outcome
- average number of filamentous fungie0
- Direction
- highere0
- Qualifier
- variation among samples was very highe0
- Comparator
- transgenic linee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the second pot experiment the average number of filamentous fungi in the rhizosphere of parent line 'Golden Promise' was higher than in the rhizosphere of transgenic line, but the variation among samples was very high probably caused by the high variation of substrate moisture content.”
t0“barley (Hordeum vulgare L.)”
Claim · model-written summary
No rhizosphere bacterial DNA sample yielded an Rpg1-specific amplification product.
- Taxon
- barleyHordeum vulgaret0
- Gene
- Rpg1e0
- Outcome
- positive amplification of Rpg1 gene-specific primer producte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“None of bacterial deoxyribonucleic acid (DNA) samples isolated from substrate of rhizosphere of barley transgenic line resulted in positive amplification of Rpg1 gene-specific primer product.”
t0“barley (Hordeum vulgare L.)”