Abstract
Triploid grapes were produced by in ovulo embryo culture. Ovules were obtained from 5 crosses: Rosario Bianco (V. vinifera L. 2x) x Kyoho (V. vinifera x V. labrusca L. 4x), Katta Kourgan(V. vinifera L. 2x)x Kyoho, Kyoho x Rosario Bianco, Kyoho x Sekirei (V. vinifera L. 2x) and Kyoho x Rizamat (V. vinifera L. 2x). Most embryos were obtained from ovules cultured at veraison. The addition of indole-3-acetic acid (2.0 mg·l-1) and gibberellic acid (0.4 mg·l-1 to 1/2 MS (half strength macro-elements) medium promoted the formation and growth of embryos. Although only a few embryos germinated directly from ruptured ovules, embryos excised from cultured ovules germinated successfully by in vitro culture. This paper demonstrates that in ovulo embryo culture is expected to be an efficient method to produce triploid grapes.
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Claims 4
Claim · model-written summary
In ovulo embryo culture produced triploid grapes.
Not reportedComparatorValue
Verbatim
e0“Triploid grapes were produced by in ovulo embryo culture”
Claim · model-written summary
Most embryos were obtained from ovules cultured at veraison.
Verbatim
e0“Most embryos were obtained from ovules cultured at veraison”
t0“grapes”
Claim · model-written summary
Indole-3-acetic acid and gibberellic acid promoted embryo formation and growth.
- Taxon
- grapest0
- Treatment
- indole-3-acetic acid (2.0 mg·l-1) and gibberellic acid (0.4 mg·l-1e0
- Outcome
- formation and growth of embryose0
- Direction
- promotede0
Not reportedComparator
Verbatim
e0“The addition of indole-3-acetic acid (2.0 mg·l-1) and gibberellic acid (0.4 mg·l-1 to 1/2 MS (half strength macro-elements) medium promoted the formation and growth of embryos”
t0“grapes”
Claim · model-written summary
Excised embryos germinated successfully in vitro, while few germinated from ruptured ovules.
Verbatim
e0“Although only a few embryos germinated directly from ruptured ovules, embryos excised from cultured ovules germinated successfully by in vitro culture”
t0“grapes”
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Gibberellic acid · growth · VitisResults differ
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See all 34 papers →indole-3-acetic acid · growth · VitisResults agree
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See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.