Abstract
Irrigation of paddy fields to arsenic (As) containing groundwater leads to As accumulation in rice grains and causes serious health risk to the people worldwide. To reduce As intake via consumption of contaminated rice grain, identification of the mechanisms for As accumulation and detoxification in rice is a prerequisite. Herein, we report involvement of a member of rice NRAMP (Natural Resistance-Associated Macrophage Protein) transporter, OsNRAMP1, in As, in addition to cadmium (Cd), accumulation through expression in yeast and Arabidopsis. Expression of OsNRAMP1 in yeast mutant (fet3fet4) rescued iron (Fe) uptake and exhibited enhanced accumulation of As and Cd. Expression of OsNRAMP1 in Arabidopsis provided tolerance with enhanced As and Cd accumulation in root and shoot. Cellular localization revealed that OsNRAMP1 resides on plasma membrane of endodermis and pericycle cells and may assist in xylem loading for root to shoot mobilization. This is the first report demonstrating role of NRAMP in xylem mediated loading and enhanced accumulation of As and Cd in plants. We propose that genetic modification of OsNRAMP1 in rice might be helpful in developing rice with low As and Cd content in grain and minimize the risk of food chain contamination to these toxic metals.
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Claims 7
Claim · model-written summary
OsNRAMP1 expression rescued iron uptake in a yeast mutant.
- Taxon
- rice
Oryza sativat0 - Gene
- OsNRAMP1e0
- Outcome
- iron (Fe) uptakee0
- Direction
- rescuede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of OsNRAMP1 in yeast mutant (fet3fet4) rescued iron (Fe) uptake”
t0“rice NRAMP, OsNRAMP1”
Claim · model-written summary
OsNRAMP1 expression increased arsenic and cadmium accumulation in yeast.
- Taxon
- riceOryza sativat0
- Gene
- OsNRAMP1e0
- Outcome
- accumulation of As and Cde0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of OsNRAMP1 in yeast mutant (fet3fet4) rescued iron (Fe) uptake and exhibited enhanced accumulation of As and Cd”
t0“rice NRAMP, OsNRAMP1”
Claim · model-written summary
OsNRAMP1 expression gave Arabidopsis tolerance to arsenic and cadmium.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Expression of OsNRAMP1e0
- Gene
- OsNRAMP1e0
- Outcome
- tolerancee0
- Direction
- providede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of OsNRAMP1 in Arabidopsis provided tolerance with enhanced As and Cd accumulation in root and shoot”
Claim · model-written summary
OsNRAMP1 expression increased arsenic and cadmium accumulation in Arabidopsis roots and shoots.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- root and shoote0
- Treatment
- Expression of OsNRAMP1e0
- Gene
- OsNRAMP1e0
- Outcome
- As and Cd accumulatione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of OsNRAMP1 in Arabidopsis provided tolerance with enhanced As and Cd accumulation in root and shoot”
Claim · model-written summary
OsNRAMP1 localized to plasma membranes of endodermal and pericycle cells.
- Taxon
- riceOryza sativat0
- Organ / stage
- endodermis and pericycle cellse0
- Gene
- OsNRAMP1e0
- Outcome
- resides on plasma membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsNRAMP1 resides on plasma membrane of endodermis and pericycle cells”
t0“rice NRAMP, OsNRAMP1”
Claim · model-written summary
The authors suggest OsNRAMP1 may assist xylem loading for root-to-shoot transport.
- Taxon
- riceOryza sativat0
- Gene
- OsNRAMP1e0
- Outcome
- xylem loading for root to shoot mobilizatione0
- Qualifier
- may assiste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsNRAMP1 resides on plasma membrane of endodermis and pericycle cells and may assist in xylem loading for root to shoot mobilization”
t0“rice NRAMP, OsNRAMP1”
Claim · model-written summary
The authors propose altering OsNRAMP1 in rice to lower grain arsenic and cadmium.
- Taxon
- riceOryza sativae0
- Gene
- OsNRAMP1e0
- Outcome
- rice with low As and Cd content in graine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genetic modification of OsNRAMP1 in rice might be helpful in developing rice with low As and Cd content in grain”
The same questions in other papers
transgenic · disease · ArabidopsisPositive and negative results
35 papers in the same direction as this paper · 19 in the opposite direction
- no effect
- negative
- negative
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See all 55 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.