Abstract
Methylglyoxal (MG) is a toxic metabolite produced primarily as a byproduct of glycolysis. Being a potent glycating agent, it can readily bind macromolecules like DNA, RNA, or proteins, modulating their expression and activity. In plants, despite the known inhibitory effects of MG on growth and development, still limited information is available about the molecular mechanisms and response pathways elicited upon elevation in MG levels. To gain insight into the molecular basis of MG response, we have investigated changes in global gene expression profiles in rice upon exposure to exogenous MG using GeneChip microarrays. Initially, growth of rice seedlings was monitored in response to increasing MG concentrations which could retard plant growth in a dose-dependent manner. Upon exposure to 10 mM concentration of MG, a total of 1685 probe sets were up- or down-regulated by more than 1.5-fold in shoot tissues within 16 h. These were classified into 10 functional categories. The genes involved in signal transduction such as, protein kinases and transcription factors, were significantly over-represented in the perturbed transcriptome, of which several are known to be involved in abiotic and biotic stress response indicating a cross-talk between MG-responsive and stress-responsive signal transduction pathways. Through in silico studies, we could predict 7-8 bp long conserved motif as a possible MG-responsive element (MGRE) in the 1 kb upstream region of genes that were more than 10-fold up- or down-regulated in the analysis. Since several perturbations were found in signaling cascades in response to MG, we hereby suggest that it plays an important role in signal transduction probably acting as a stress signal molecule.
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Claims 5
Claim · model-written summary
Increasing exogenous methylglyoxal concentrations retarded rice seedling growth in a dose-dependent manner.
- Taxon
- rice
Oryza sativae0 - Treatment
- increasing MG concentrationse0
- Outcome
- plant growthe0
- Direction
- retarde0
- Qualifier
- dose-dependent mannere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“growth of rice seedlings was monitored in response to increasing MG concentrations which could retard plant growth in a dose-dependent manner”
Claim · model-written summary
Exposure of rice to 10 mM methylglyoxal up- or down-regulated 1685 probe sets by more than 1.5-fold in shoot tissues within 16 h.
- Taxon
- riceOryza sativat0
- Organ / stage
- shoot tissuese0
- Treatment
- exposure to 10 mM concentration of MGe0
- Outcome
- probe setse0
- Direction
- up- or down-regulatede0
- Value
- 1685e0
- Qualifier
- more than 1.5-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Upon exposure to 10 mM concentration of MG, a total of 1685 probe sets were up- or down-regulated by more than 1.5-fold in shoot tissues within 16 h”
t0“rice”
Claim · model-written summary
Genes involved in signal transduction, such as protein kinases and transcription factors, were significantly over-represented in the methylglyoxal-perturbed rice transcriptome.
- Taxon
- riceOryza sativat0
- Outcome
- genes involved in signal transductione0
- Direction
- over-representede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The genes involved in signal transduction such as, protein kinases and transcription factors, were significantly over-represented in the perturbed transcriptome”
t0“rice”
Claim · model-written summary
In silico analysis predicted a 7-8 bp conserved motif as a possible methylglyoxal-responsive element in the 1 kb upstream region of strongly regulated genes.
- Taxon
- riceOryza sativat0
- Outcome
- 7-8 bp long conserved motif as a possible MG-responsive element (MGRE)e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we could predict 7-8 bp long conserved motif as a possible MG-responsive element (MGRE) in the 1 kb upstream region of genes”
t0“rice”
Claim · model-written summary
The authors suggest that methylglyoxal plays an important role in signal transduction, probably acting as a stress signal molecule.
- Taxon
- riceOryza sativat0
- Outcome
- signal transductione0
- Qualifier
- stress signal moleculee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we hereby suggest that it plays an important role in signal transduction probably acting as a stress signal molecule”
t0“rice”
The same questions in other papers
methylglyoxal · growth · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.