Abstract
In tuber-bearing Solanum species (potatoes), S-locus mediated self-incompatibility is under gametophytic control. In this system, self-fertilization is avoided when the same S-allele is expressed in both pistil and pollen. Because the ultrastructural details of the self-incompatibility response in this group of species is unknown, the aim of this study was to identify the cellular events involved in this response by using the diploid species S. chacoense Bitter as a model. To this end, pollinations were carried out in two genotypic combinations previously identified as (1) compatible and (2) self-incompatible (self-pollination) for the S-locus. Pollinated pistils were fixed on an hourly basis and observed under a transmission electron microscope to detect ultrastructural changes. In the self-incompatible genotypic combination, pollen grains germinated normally but loss of electron density in the mitochondrial matrix of pollen tubes was observed at 1 hour after pollination (HAP), mitochondrial swelling started at 2 HAP and, finally, mitochondrial content was lost at 7 HAP, with the concomitant cessation of pollen tube growth in the upper third of the style. The gametophytic self-incompatibility response in potato shares similarities with a type of programmed cell death.
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Claims 6
Claim · model-written summary
Loss of electron density in the mitochondrial matrix of pollen tubes was observed at 1 hour after pollination in the self-incompatible combination.
- Taxon
- Solanum chacoense
Solanum chacoenset0 - Organ / stage
- pollen tubese1
- Outcome
- loss of electron density in the mitochondrial matrixe1
- Qualifier
- 1 hour after pollination (HAP)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the wild potato Solanum chacoense bitter”
e1“loss of electron density in the mitochondrial matrix of pollen tubes was observed at 1 hour after pollination (HAP)”
Claim · model-written summary
Mitochondrial swelling started at 2 hours after pollination in the self-incompatible combination.
- Taxon
- Solanum chacoenseSolanum chacoenset0
- Outcome
- mitochondrial swellinge2
- Qualifier
- 2 HAPe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the wild potato Solanum chacoense bitter”
e2“mitochondrial swelling started at 2 HAP”
Claim · model-written summary
Mitochondrial content was lost at 7 hours after pollination in the self-incompatible combination.
- Taxon
- Solanum chacoenseSolanum chacoenset0
- Outcome
- mitochondrial contente3
- Qualifier
- 7 HAPe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the wild potato Solanum chacoense bitter”
e3“mitochondrial content was lost at 7 HAP”
Claim · model-written summary
Pollen tube growth ceased in the upper third of the style in the self-incompatible combination.
- Taxon
- Solanum chacoenseSolanum chacoenset0
- Organ / stage
- upper third of the stylee4
- Outcome
- pollen tube growthe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the wild potato Solanum chacoense bitter”
e4“with the concomitant cessation of pollen tube growth in the upper third of the style”
Claim · model-written summary
Pollen grains germinated normally in the self-incompatible combination.
- Taxon
- Solanum chacoenseSolanum chacoenset0
- Outcome
- pollen grains germinatede5
- Qualifier
- normallye5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the wild potato Solanum chacoense bitter”
e5“pollen grains germinated normally”
Claim · model-written summary
The gametophytic self-incompatibility response in potato is suggested to resemble a type of programmed cell death.
- Taxon
- potato
Solanum tuberosume6 - Outcome
- gametophytic self-incompatibility responsee6
- Qualifier
- shares similarities with a type of programmed cell deathe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“The gametophytic self-incompatibility response in potato shares similarities with a type of programmed cell death”