Abstract
The effects of phytochrome/light and other regulatory agents on the swelling of protoplasts from the primary leaves of etiolated oat seedlings have been investigated. The protoplasts did not swell in darkness. Red (R) light immediately followed by far-red (FR) or FR light treatment alone for 4 h induced swelling slowly. In comparison, the protoplasts treated with R or FR-R swell more rapidly and with a shorter lag period. The effect of red light on the protoplast swelling was photoreversible by FR, suggesting the involvement of endogenous phytochrome. Exogenous gibberellic acid (GA3) or dibutyryl cAMP (DBcAMP) stimulated the effect of R irradiation on the protoplast swelling. As with R irradiation, these agents were sufficient to cause the swelling of protoplasts in the dark with a shorter lag period than those maintained in darkness or a 5 min FR. The combination of a 5 min R irradiation and GA3 showed a synergistic effect on the enlargement of protoplast size. On the other hand, the increase in protoplast size was proportional to the concentration of DBcAMP with or without a 5 min R irradiation.
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Claims 8
Claim · model-written summary
Oat protoplasts did not swell in darkness.
- Taxon
- Oat
Avena sativat0 - Outcome
- swelle0
- Direction
- did not swelle0
- Setting
- darknesse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The protoplasts did not swell in darkness”
t0“Oat Protoplasts”
Claim · model-written summary
Red followed by far-red light, or far-red alone for four hours, induced slow swelling.
- Taxon
- OatAvena sativat0
- Treatment
- Red (R) light immediately followed by far-red (FR) or FR light treatment alone for 4 he0
- Outcome
- swellinge0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Red (R) light immediately followed by far-red (FR) or FR light treatment alone for 4 h induced swelling slowly”
t0“Oat Protoplasts”
Claim · model-written summary
Red light alone or far-red followed by red produced more rapid swelling and a shorter lag.
- Taxon
- OatAvena sativat0
- Treatment
- R or FR-Re0
- Outcome
- swell more rapidly and with a shorter lag periode0
- Direction
- more rapidlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In comparison, the protoplasts treated with R or FR-R swell more rapidly and with a shorter lag period”
t0“Oat Protoplasts”
Claim · model-written summary
Far-red light reversed the effect of red light on protoplast swelling.
- Taxon
- OatAvena sativat0
- Treatment
- FRe0
- Outcome
- effect of red light on the protoplast swellinge0
- Direction
- photoreversiblee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effect of red light on the protoplast swelling was photoreversible by FR”
t0“Oat Protoplasts”
Claim · model-written summary
Gibberellic acid stimulated the effect of red light on oat protoplast swelling.
- Taxon
- OatAvena sativat0
- Treatment
- gibberellic acid (GA3)e0
- Outcome
- protoplast swellinge0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous gibberellic acid (GA3) or dibutyryl cAMP (DBcAMP) stimulated the effect of R irradiation on the protoplast swelling”
t0“Oat Protoplasts”
Claim · model-written summary
Dibutyryl cAMP stimulated the effect of red light on oat protoplast swelling.
- Taxon
- OatAvena sativat0
- Treatment
- dibutyryl cAMP (DBcAMP)e0
- Outcome
- protoplast swellinge0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous gibberellic acid (GA3) or dibutyryl cAMP (DBcAMP) stimulated the effect of R irradiation on the protoplast swelling”
t0“Oat Protoplasts”
Claim · model-written summary
Gibberellic acid and red light had a synergistic effect on protoplast enlargement.
- Taxon
- OatAvena sativat0
- Treatment
- 5 min R irradiation and GA3e0
- Outcome
- enlargement of protoplast sizee0
- Direction
- synergistic effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The combination of a 5 min R irradiation and GA3 showed a synergistic effect on the enlargement of protoplast size”
t0“Oat Protoplasts”
Claim · model-written summary
Oat protoplast size increased in proportion to DBcAMP concentration with or without red light.
- Taxon
- OatAvena sativat0
- Treatment
- concentration of DBcAMPe0
- Outcome
- protoplast sizee0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the increase in protoplast size was proportional to the concentration of DBcAMP with or without a 5 min R irradiation”
t0“Oat Protoplasts”
The same questions in other papers
Gibberellic acid · growth · Avena sativa (oats)Positive and negative results
16 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
14 papers more in this group
See all 18 papers →radiation · growth · Avena sativa (oats)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.