Abstract
The human Bcl-xL gene was transformed into peanut cultivar Georgia Green via microprojectile bombardment. Following selection on hygromycin-containing medium and regeneration, eighty hygromycin-resistant callus clusters were recovered. Southern blot analysis of ten fertile lines revealed multiple insertions of the Bcl-xL transgene in most lines. Western blot analysis of primary plants and T1 progenies demonstrated detectable levels of Bcl-xL expression in four transgenic lines. We could not detect Bcl-xL protein in other tested lines even though transcripts were identified by RT-PCR and northern blot. Three of the western-positive transgenic lines either were sterile or the progenies lost the expressive copy of Bcl-xL. Only T1 progenies from line BX25-4-2a-19 continued to express an intermediate level of Bcl-xL. This line demonstrated paraquat tolerance at the 5 microM level. Tolerance to salt of T1 and T2 seeds from seven other transgenic lines also was tested, but no tolerance was found in these lines. A high level of Bcl-xL transgene expression may be deleterious to plant growth and development even though the gene may confer tolerance to other abiotic and biotic stresses such as drought and pathogens.
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Claims 10
Claim · model-written summary
Eighty hygromycin-resistant callus clusters were recovered after selection and regeneration of peanut.
- Taxon
- peanut
Arachis hypogaeae2 - Material
- Georgia Greene2
- Outcome
- hygromycin-resistant callus clusterse1
- Value
- eightye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“eighty hygromycin-resistant callus clusters were recovered”
e2“peanut cultivar Georgia Green”
Claim · model-written summary
Southern blot analysis of ten fertile lines showed multiple insertions of the Bcl-xL transgene in most lines.
- Taxon
- peanutArachis hypogaeae2
- Material
- fertile linese1
- Gene
- Bcl-xLe1
- Outcome
- multiple insertions of the Bcl-xL transgenee1
- Qualifier
- in most linese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Southern blot analysis of ten fertile lines revealed multiple insertions of the Bcl-xL transgene in most lines”
e2“peanut cultivar Georgia Green”
Claim · model-written summary
Western blot detected Bcl-xL expression at detectable levels in four transgenic lines among primary plants and T1 progenies.
- Taxon
- peanutArachis hypogaeae2
- Organ / stage
- primary plants and T1 progeniese1
- Gene
- Bcl-xLe1
- Outcome
- detectable levels of Bcl-xL expressione1
- Value
- four transgenic linese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Western blot analysis of primary plants and T1 progenies demonstrated detectable levels of Bcl-xL expression in four transgenic lines”
e2“peanut cultivar Georgia Green”
Claim · model-written summary
Bcl-xL protein could not be detected in other tested peanut lines.
- Taxon
- peanutArachis hypogaeae2
- Gene
- Bcl-xL proteine1
- Outcome
- Bcl-xL proteine1
- Qualifier
- other tested linese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We could not detect Bcl-xL protein in other tested lines”
e2“peanut cultivar Georgia Green”
Claim · model-written summary
Transcripts were identified by RT-PCR and northern blot even though Bcl-xL protein was not detected in other tested peanut lines.
- Taxon
- peanutArachis hypogaeae2
- Gene
- Bcl-xL proteine3
- Outcome
- transcriptse1
- Qualifier
- RT-PCR and northern blote1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“even though transcripts were identified by RT-PCR and northern blot”
e3“We could not detect Bcl-xL protein in other tested lines”
e2“peanut cultivar Georgia Green”
Claim · model-written summary
Three western-positive transgenic peanut lines were either sterile or their progenies lost the expressive copy of Bcl-xL.
- Taxon
- peanutArachis hypogaeae2
- Material
- western-positive transgenic linese1
- Gene
- Bcl-xLe1
- Outcome
- sterile or the progenies lost the expressive copye1
- Value
- Threee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Three of the western-positive transgenic lines either were sterile or the progenies lost the expressive copy of Bcl-xL”
e2“peanut cultivar Georgia Green”
Claim · model-written summary
Only T1 progenies from line BX25-4-2a-19 continued to express an intermediate level of Bcl-xL.
- Taxon
- peanutArachis hypogaeae2
- Material
- line BX25-4-2a-19e1
- Organ / stage
- T1 progeniese1
- Gene
- Bcl-xLe1
- Outcome
- continued to express an intermediate level of Bcl-xLe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Only T1 progenies from line BX25-4-2a-19 continued to express an intermediate level of Bcl-xL”
e2“peanut cultivar Georgia Green”
Claim · model-written summary
Line BX25-4-2a-19 showed paraquat tolerance at the 5 microM level.
- Taxon
- peanutArachis hypogaeae2
- Material
- line BX25-4-2a-19e3
- Treatment
- paraquate1
- Outcome
- paraquat tolerancee1
- Value
- 5 microMe1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“This line demonstrated paraquat tolerance at the 5 microM level”
e3“line BX25-4-2a-19”
e2“peanut cultivar Georgia Green”
Claim · model-written summary
No salt tolerance was found in T1 and T2 seeds of seven other transgenic peanut lines.
- Taxon
- peanutArachis hypogaeae2
- Material
- seven other transgenic linese1
- Organ / stage
- T1 and T2 seedse1
- Treatment
- salte1
- Outcome
- Tolerance to salte1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Tolerance to salt of T1 and T2 seeds from seven other transgenic lines also was tested, but no tolerance was found in these lines”
e2“peanut cultivar Georgia Green”
Claim · model-written summary
High Bcl-xL transgene expression may reduce peanut plant growth and development.
- Taxon
- peanutArachis hypogaeae2
- Treatment
- A high level of Bcl-xL transgene expressione1
- Gene
- Bcl-xLe1
- Outcome
- plant growth and developmente1
- Direction
- decreaseinferrede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A high level of Bcl-xL transgene expression may be deleterious to plant growth and development”
e2“peanut cultivar Georgia Green”
The same questions in other papers
paraquat · disease · Arachis hypogaea (peanut)Positive and negative results
1 in the opposite direction
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.