Abstract
1. Some findings an the nucleic acids of Feulgen negative plants, Oscillatoria limosa, Spirogyra sp. and Marchantia polymorpha, studied by the Feulgen's staining method and biochemical procedures, are reported.2. Cells of these plants were not stained with Feulgen's method under various conditions of fixations and acid hydrolysis, but certain amounts of DNA were isolated biochemically from cells of these plants. Isolated DNA gives positive reaction with Feulgen's and Dische's tests in vitro. Absorption maximum of the Feulgen's color at pH 2.28 with DNA from these plants, locates at 565mμ.3. Maximum of UV-absorption curve of DNA, isolated by Schmidt and Thannhauser's method from above plants, situates at 260mμ in all cases, and no significant difference among UV-absorption curves of DNA from these plants and calf thymus, has been found. UV-absorption maximum of DNA, isolated by the procedure of Ogur and Rosen using hot PCA, however, locates at 268mμ. Similar results are obtained when calf thumus and fish sperm-DNAs have been heated with hot PCA.4. Nucleic acids and other P-containing substances in cells of these Feulgen negative plants are determined quantitatively. Especially, DNA and RNA have been determined by several methods for the estimation of three major constituents of nucleic acids. Mean values of DNA content per cell in Oscillatoria limosa, Spirogyra sp. and Marchantia polymorpha are 8.1, 2.9 and 0.48×10-9mg respectively.Causal analyses of Feulgen negativity in histochemical preparations will be discussed in another paper.
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Claims 9
Claim · model-written summary
Cells of Feulgen-negative plants were not stained by Feulgen method under various fixation and acid hydrolysis conditions.
Verbatim
e0“Feulgen negative plants, Oscillatoria limosa, Spirogyra sp. and Marchantia polymorpha”
e1“Cells of these plants were not stained with Feulgen's method under various conditions of fixations and acid hydrolysis”
Claim · model-written summary
DNA isolated from these plants gives a positive reaction with Feulgen and Dische tests in vitro.
Verbatim
e0“Feulgen negative plants, Oscillatoria limosa, Spirogyra sp. and Marchantia polymorpha”
e2“Isolated DNA gives positive reaction with Feulgen's and Dische's tests in vitro”
Claim · model-written summary
The absorption maximum of the Feulgen color of DNA from these plants at pH 2.28 is 565 mμ.
Verbatim
e0“Feulgen negative plants, Oscillatoria limosa, Spirogyra sp. and Marchantia polymorpha”
e3“Absorption maximum of the Feulgen's color at pH 2.28 with DNA from these plants, locates at 565mμ”
Claim · model-written summary
The UV-absorption maximum of DNA isolated from these plants is 260 mμ in all cases.
Verbatim
e0“Feulgen negative plants, Oscillatoria limosa, Spirogyra sp. and Marchantia polymorpha”
e4“Maximum of UV-absorption curve of DNA, isolated by Schmidt and Thannhauser's method from above plants, situates at 260mμ in all cases”
Claim · model-written summary
The UV-absorption curves of DNA from these plants show no significant difference from calf thymus DNA.
Verbatim
e0“Feulgen negative plants, Oscillatoria limosa, Spirogyra sp. and Marchantia polymorpha”
e5“no significant difference among UV-absorption curves of DNA from these plants and calf thymus, has been found”
Claim · model-written summary
The UV-absorption maximum of DNA isolated by the Ogur and Rosen procedure with hot PCA is 268 mμ.
Verbatim
e0“Feulgen negative plants, Oscillatoria limosa, Spirogyra sp. and Marchantia polymorpha”
e6“UV-absorption maximum of DNA, isolated by the procedure of Ogur and Rosen using hot PCA, however, locates at 268mμ”
Claim · model-written summary
Mean DNA content per cell in Oscillatoria limosa is 8.1 ×10-9 mg.
- Taxon
- Oscillatoria limosaOscillatoria limosae7
- Outcome
- DNA content per celle7
- Value
- 8.1e7
- Qualifier
- ×10-9mge7
Verbatim
e7“Mean values of DNA content per cell in Oscillatoria limosa, Spirogyra sp. and Marchantia polymorpha are 8.1, 2.9 and 0.48×10-9mg respectively”
Claim · model-written summary
Mean DNA content per cell in Spirogyra sp. is 2.9 ×10-9 mg.
SpirogyraOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Mean values of DNA content per cell in Oscillatoria limosa, Spirogyra sp. and Marchantia polymorpha are 8.1, 2.9 and 0.48×10-9mg respectively”
Claim · model-written summary
Mean DNA content per cell in Marchantia polymorpha is 0.48 ×10-9 mg.
- Taxon
- Marchantia polymorpha
Marchantia polymorphae7 - Outcome
- DNA content per celle7
- Value
- 0.48e7
- Qualifier
- ×10-9mge7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Mean values of DNA content per cell in Oscillatoria limosa, Spirogyra sp. and Marchantia polymorpha are 8.1, 2.9 and 0.48×10-9mg respectively”