Abstract
Actin filaments and chloroplasts in guard cells play roles in stomatal function. However, detailed actin dynamics vary, and the roles that they play in chloroplast localization during stomatal movement remain to be determined. We examined the dynamics of actin filaments and chloroplast localization in transgenic tobacco expressing green fluorescent protein (GFP)-mouse talin in guard cells by time-lapse imaging. Actin filaments showed sliding, bundling and branching dynamics in moving guard cells. During stomatal movement, long filaments can be severed into small fragments, which can form longer filaments by end-joining activities. With chloroplast movement, actin filaments near chloroplasts showed severing and elongation activity in guard cells during stomatal movement. Cytochalasin B treatment abolished elongation, bundling and branching activities of actin filaments in guard cells, and these changes of actin filaments, and as a result, more chloroplasts were localized at the centre of guard cells. However, chloroplast turning to avoid high light, and sliding of actin fragments near the chloroplast, was unaffected following cytochalasin B treatment in guard cells. We suggest that the sliding dynamics of actin may play roles in chloroplast turning in guard cells. Our results indicate that the stochastic dynamics of actin filaments in guard cells regulate chloroplast localization during stomatal movement.
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Claims 9
Claim · model-written summary
Actin filaments showed sliding, bundling and branching in moving guard cells.
- Taxon
- tobacco
Nicotiana tabacume1 - Organ / stage
- guard cellse0
- Outcome
- sliding, bundling and branching dynamicse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Actin filaments showed sliding, bundling and branching dynamics in moving guard cells.”
e1“transgenic tobacco expressing green fluorescent protein (GFP)-mouse talin in guard cells”
Claim · model-written summary
Long actin filaments were severed into fragments during stomatal movement.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- severed into small fragmentse0
- Qualifier
- During stomatal movemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During stomatal movement, long filaments can be severed into small fragments”
e1“transgenic tobacco”
Claim · model-written summary
Actin fragments could form longer filaments by end joining.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- form longer filaments by end-joining activitiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which can form longer filaments by end-joining activities.”
e1“transgenic tobacco”
Claim · model-written summary
Actin near moving chloroplasts showed severing and elongation during stomatal movement.
- Taxon
- tobaccoNicotiana tabacume1
- Organ / stage
- guard cellse0
- Outcome
- severing and elongation activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“With chloroplast movement, actin filaments near chloroplasts showed severing and elongation activity in guard cells during stomatal movement.”
e1“transgenic tobacco”
Claim · model-written summary
Cytochalasin B abolished actin elongation, bundling and branching in guard cells.
- Taxon
- tobaccoNicotiana tabacume1
- Organ / stage
- guard cellse0
- Treatment
- Cytochalasin B treatmente0
- Outcome
- elongation, bundling and branching activities of actin filamentse0
- Direction
- abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cytochalasin B treatment abolished elongation, bundling and branching activities of actin filaments in guard cells”
e1“transgenic tobacco”
Claim · model-written summary
Cytochalasin B treatment resulted in more chloroplasts at the guard-cell centre.
- Taxon
- tobaccoNicotiana tabacume2
- Treatment
- Cytochalasin B treatmente1
- Outcome
- chloroplasts were localized at the centre of guard cellse0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“more chloroplasts were localized at the centre of guard cells.”
e1“Cytochalasin B treatment”
e2“transgenic tobacco”
Claim · model-written summary
Cytochalasin B did not affect chloroplast turning to avoid high light or nearby actin-fragment sliding.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- cytochalasin B treatmente0
- Outcome
- chloroplast turning to avoid high light, and sliding of actin fragments near the chloroplaste0
- Direction
- unaffectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“chloroplast turning to avoid high light, and sliding of actin fragments near the chloroplast, was unaffected following cytochalasin B treatment in guard cells.”
e1“transgenic tobacco”
Claim · model-written summary
The authors suggest actin sliding may contribute to chloroplast turning in guard cells.
- Taxon
- tobaccoNicotiana tabacume1
- Organ / stage
- guard cellse0
- Outcome
- chloroplast turninge0
- Qualifier
- sliding dynamics of actine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We suggest that the sliding dynamics of actin may play roles in chloroplast turning in guard cells.”
e1“transgenic tobacco”
Claim · model-written summary
The authors conclude stochastic actin dynamics regulate chloroplast localization during stomatal movement.
- Taxon
- tobaccoNicotiana tabacume1
- Organ / stage
- guard cellse0
- Outcome
- chloroplast localizatione0
- Qualifier
- stochastic dynamics of actin filamentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the stochastic dynamics of actin filaments in guard cells regulate chloroplast localization during stomatal movement.”
e1“transgenic tobacco”
The same questions in other papers
boron atom · growth · Nicotiana tabacum (tobacco)Positive and negative results
3 papers in the same direction as this paper · 13 in the opposite direction
- positive
- positive
- positive
13 papers more in this group
See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.